Identification of Viral Peptide Fragments for Vaccine Development [PDF]
, 2009 We report a simple method for identifying foldable viral surface protein fragments in a random but systematic manner. The method involves digestion and reassembly of a target gene to generate a pool of smaller DNA fragments with random ends but controllable lengths, followed by screening for foldable fragments using green fluorescent protein (GFP) as a
Yong Chen, Zhanglin Lin, Shuang Li
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Unusual fragmentation of derivatized cysteine‐containing peptides [PDF]
Rapid Communications in Mass Spectrometry, 2018 RationaleModification of cysteines by aminoethylation results in side chains similar to those of lysine. Trypsin cleaves at this modified residue and this labeling method can facilitate the analysis of proteins, specifically antibodies. In this work, the ability to identify peptides containing aminoethylated cysteines is investigated through digestion,
Nick Degraan‐Weber +2 more
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FEBS Letters, EarlyView.This work presents the characterization of MvoDUF2193, a Methanococcus voltae (Mvo) protein from the domain of unknown function (DUF) 2193 family. We demonstrate that MvoDUF2193 binds a single [4Fe–4S] cluster per subunit and that cluster occupancy regulates the transition from an apo tetramer to a [4Fe–4S] monomeric form. This structural transition is
Emily M. Dieter +8 more
wiley +1 more source
Determining the Hydrophobicity Index of Protected Amino Acids and Common Protecting Groups
Separations, 2023 Peptides are in great demand in the pharmaceutical arena and a majority of these peptides contain 20 or more amino acids. They are infrequently synthesised using the fragment condensation approach. A key limitation in adopting this approach more commonly
Varshitha Gavva +3 more
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Selection of gonadotrophin surge attenuating factor phage antibodies by bioassay [PDF]
, 2005 Peer reviewedPublisher ...
Fowler, Paul Alfred Francois +3 more
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Peptide fragmentation induced by radicals at atmospheric pressure [PDF]
Journal of Mass Spectrometry, 2008 AbstractA novel ion dissociation technique, which is capable of providing an efficient fragmentation of peptides at essential atmospheric pressure conditions, is developed. The fragmentation patterns observed often contain c‐type fragments that are specific to electron capture dissociation/electron transfer dissociation (ECD/ETD), along with the y‐/b ...
Andrey N. Vilkov +2 more
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Peripheral blood proteome biomarkers distinguish immunosuppressive features of cancer progression
Molecular Oncology, EarlyView.Immune status significantly influences cancer progression. This study used plasma proteomics to analyze benign 67NR and malignant 4T1 breast tumor models at early and late tumor stages. Immune‐related proteins–osteopontin (Spp1), lactotransferrin (Ltf), calreticulin (Calr) and peroxiredoxin 2 (Prdx2)–were associated with systemic myeloid‐derived ...
Yeon Ji Park +6 more
wiley +1 more source
Research Applications of Proteolytic Enzymes in Molecular Biology
Biomolecules, 2013 Proteolytic enzymes (also termed peptidases, proteases and proteinases) are capable of hydrolyzing peptide bonds in proteins. They can be found in all living organisms, from viruses to animals and humans.
József Tőzsér +2 more
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Dynorphin 1-17 and Its N-Terminal Biotransformation Fragments Modulate Lipopolysaccharide-Stimulated Nuclear Factor-kappa B Nuclear Translocation, Interleukin-1beta and Tumor Necrosis Factor-alpha in Differentiated THP-1 Cells. [PDF]
PLoS ONE, 2016 Dynorphin 1-17, (DYN 1-17) opioid peptide produces antinociception following binding to the kappa-opioid peptide (KOP) receptor. Upon synthesis and release in inflamed tissues by immune cells, DYN 1-17 undergoes rapid biotransformation and yields a ...
Siti Sarah Fazalul Rahiman +4 more
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The anti-tumor properties of two tumstatin peptide fragments in human gastric carcinoma
Acta Pharmacologica Sinica, 2009 Aim:The aim was to study the anti-tumor activities and mechanisms of two synthetic peptide fragments of tumstatin (alpha3 (IV) NC1 domain) in human gastric carcinoma cells in vitro and in vivo.Methods:MTT assay and cell cycle assay were used to study the
Ying-Jie Li +6 more
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