Partial Purification and Characterization of Two Peptide Hydrolases from Pea Seeds [PDF]
Two peptide hydrolases have been found in pea seeds (Pisum sativum var. Greenfeast) and extensively purified by ion exchange chromatography using benzoyl-dl-arginine-p-nitroanilide as substrate. The enzymes which both have molecular weights of 65,000 can
J. Bruce Caldwell, Lindsay G. Sparrow
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Dimer-monomer transition defines a hyper-thermostable peptidoglycan hydrolase mined from bacterial proteome by lysin-derived antimicrobial peptide-primed screening [PDF]
Phage-derived peptidoglycan hydrolases (i.e. lysins) are considered promising alternatives to conventional antibiotics due to their direct peptidoglycan degradation activity and low risk of resistance development.
Li Zhang+11 more
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Evidence for two different modes of tripeptide disappearance in human intestine. Uptake by peptide carrier systems and hydrolysis by peptide hydrolases. [PDF]
The intestinal fate of two tripeptides (triglycine and trileucine), which differ markedly in solubility and molecular weight, have been investigated by jejunal perfusion in healthy human volunteers.
Siamak A. Adibi+3 more
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EFFECT OF PROTEASE INHIBITORS OF ACTINOMYCETES ON LYSOSOMAL PEPTIDE-HYDROLASES FROM SWINE LIVER
small pieces, homogenized with two volumes of 50mMTris buffer (pH 7.4) containing 0.25 Msucrose and 1 mMEDTA, and centrifuged at 700g for 10minutes. After removal of the precipitate (nuclear-debris), the supernatant was further centrifuged at 7,000g for ...
Hiroh Ikezawa+3 more
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Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium [PDF]
Two well-characterized enzymes in Salmonella entericaserovar Typhimurium and Escherichia coli are able to hydrolyze N-terminal aspartyl (Asp) dipeptides: peptidase B, a broad-specificity aminopeptidase, and peptidase E, an Asp-specific dipeptidase.
Rachel A. Larsen+2 more
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The intracellular peptide-hydrolases of Lactobacillus plantarum. Comparison with Lactobacillus casei [PDF]
Summary Although Lactobacillus plant arum has been isolated in large numbers from ripenedcheeses, the knowledge of its proteolytic system is very limited.
M. El Soda+4 more
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The cell wall (CW) of bacteria is an intricate arrangement of macromolecules, at least constituted of peptidoglycan (PG) but also of (lipo)teichoic acids, various polysaccharides, polyglutamate and/or proteins. During bacterial growth and division, there
Régine Talon+2 more
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EFFECT OF ANTIPAIN ON LYSOSOMAL PEPTIDE-HYDROLASES FROM SWINE LIVER
In the previous report9) the authors examined the effect of leupeptin, pepstatin and chymostatin on cathepsins A, B and D. In the present study, the effect of antipain on cathepsins was investigated in comparison with the inhibitors mentioned above.
Hiroh Ikezawa+4 more
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Effect of nitrogen on activity of peptide hydrolases and protein content in caryopses and flag leaf of barley in ontogenesis [PDF]
The aim of the research was to study the influence peculiarities of different levels of nitrogen nutrition on the activity of peptide hydrolases and protein content in ontogeny of barley plants.The paper discusses the content of total nitrogen, protein ...
Gamzaeva R S
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The Human Ntn-Hydrolase Superfamily: Structure, Functions and Perspectives
N-terminal nucleophile (Ntn)-hydrolases catalyze the cleavage of amide bonds in a variety of macromolecules, including the peptide bond in proteins, the amide bond in N-linked protein glycosylation, and the amide bond linking a fatty acid to sphingosine ...
Arne Linhorst, Torben Lübke
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