Results 31 to 40 of about 20,249 (205)

The Molecular Basis for Escherichia coli O157:H7 Phage FAHEc1 Endolysin Function and Protein Engineering to Increase Thermal Stability

Viruses, 2021
Bacteriophage-encoded endolysins have been identified as antibacterial candidates. However, the development of endolysins as mainstream antibacterial agents first requires a comprehensive biochemical understanding. This study defines the atomic structure
Michael J. Love, David Coombes, Sarah H. Manners, Gayan S. Abeysekera, Craig Billington, Renwick C. J. Dobson   +5 more
doaj   +1 more source

Bacteriophage-encoded virion-associated enzymes to overcome the carbohydrate barriers during the infection process [PDF]

, 2017
Bacteriophages are bacterial viruses that infect the host after successful receptor recognition and adsorption to the cell surface. The irreversible adherence followed by genome material ejection into host cell cytoplasm must be preceded by the passage ...
Briers, Yves, Drulis-Kawa, Zuzanna, Maciejewska, Barbara, Majkowska-Skrobek, Grazyna, Łątka, Agnieszka   +4 more
core   +1 more source

Bacterial growth does require peptidoglycan hydrolases [PDF]

Molecular Microbiology, 2012
SummaryMost bacteria surround their cytoplasmic membrane with a net‐like, elastic heteropolymer, the peptidoglycan sacculus, to protect themselves from bursting due to the turgor and to maintain cell shape. It has been assumed that growing bacteria require peptidoglycan hydrolases to open meshes in the peptidoglycan net allowing the insertion of the ...
openaire   +2 more sources

Bacteriophage virion-associated peptidoglycan hydrolases: potential new enzybiotics [PDF]

Critical Reviews in Microbiology, 2012
Virion-associated peptidoglycan hydrolases (VAPGH) are phage-encoded lytic enzymes that locally degrade the peptidoglycan (PG) of the bacterial cell wall during infection. In contrast to endolysins, PGHs that mediate lysis of the host bacteria at the end of the lytic cycle to release of phage progeny, the action of VAPGHs generates a small hole through
Rodríguez, Lorena, Martínez Fernández, Beatriz, Donovan, David M., Rodríguez González, Ana, García Suárez, María Pilar   +4 more
openaire   +3 more sources

In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA). [PDF]

PLoS Pathogens, 2017
The O-acetylation of the essential cell wall polymer peptidoglycan occurs in most Gram-positive bacterial pathogens, including species of Staphylococcus, Streptococcus and Enterococcus. This modification to peptidoglycan protects these pathogens from the
David Sychantha, Carys S Jones, Dustin J Little, Patrick J Moynihan, Howard Robinson, Nicola F Galley, David I Roper, Christopher G Dowson, P Lynne Howell, Anthony J Clarke   +9 more
doaj   +1 more source

Substrate-Assisted Catalysis Unifies Two Families of Chitinolytic Enzymes [PDF]

, 1997
Hen egg-white lysozyme has long been the paradigm for enzymatic glycosyl hydrolysis with retention of configuration, with a protonated carboxylic acid and a deprotonated carboxylate participating in general acid-base catalysis.
Blake C. C. F., C−, Davies G. J., Ford L. O., Henrissat B., Iseli B., Johnson L. N., Jones T. A., Kirby A., Knapp S., Kobayashi S., Koshland D. E., Perrakis A., Phillips D. C., Piszkiewicz D., Rao V. H., Rozeboom H. J., Sinnot M. L., Strynadka N. C. J., Sulzenbacher G., Terwisscha van Scheltinga A. C., Terwisscha van Scheltinga A. C., Terwisscha van Scheltinga A. C., Tews I., Thunnissen A. M. W. H., Tronrud D. E., van Roey P., Vårum K. M., Weaver L. H.   +28 more
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Peptidoglycan Hydrolases of Escherichia coli [PDF]

Microbiology and Molecular Biology Reviews, 2011
SUMMARYThe review summarizes the abundant information on the 35 identified peptidoglycan (PG) hydrolases ofEscherichia coliclassified into 12 distinct families, including mainly glycosidases, peptidases, and amidases. An attempt is also made to critically assess their functions in PG maturation, turnover, elongation, septation, and recycling as well as
openaire   +2 more sources

Bacterial Cell Enlargement Requires Control of Cell Wall Stiffness Mediated by Peptidoglycan Hydrolases. [PDF]

, 2015
Most bacterial cells are enclosed in a single macromolecule of the cell wall polymer, peptidoglycan, which is required for shape determination and maintenance of viability, while peptidoglycan biosynthesis is an important antibiotic target.
Bailey, R.G., Foster, S.J., Hayhurst, E.J., Hobbs, J.K., Horsburgh, M., Mesnage, S., Mohamad, S.A., Salamaga, B., Turner, R.D., Wheeler, R.   +9 more
core   +8 more sources

Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products [PDF]

, 2014
Twenty-three Lactobacillus strains of dairy origin were evaluated for some functional properties relevant to their use as probiotics. A preliminary subtractive screening based on the abilities to inhibit the growth of microbial pathogens and hydrolyze ...
Conti S, Cosentino S, Deiana M, Deplano M, Fadda ME, Melis MP, Pisano MB, Viale S   +7 more
core   +1 more source

One fold, many functions—M23 family of peptidoglycan hydrolases

Frontiers in Microbiology, 2022
Bacterial cell walls are the guards of cell integrity. They are composed of peptidoglycan that provides rigidity to sustain internal turgor and ensures isolation from the external environment. In addition, they harbor the enzymatic machinery to secure cell wall modulations needed throughout the bacterial lifespan.
Alicja Razew, Jan-Niklas Schwarz, Paweł Mitkowski, Izabela Sabala, Magdalena Kaus-Drobek   +4 more
openaire   +3 more sources