Results 11 to 20 of about 121,778 (347)

Evolution of phage display libraries for therapeutic antibody discovery

mAbs, 2023
Monoclonal antibodies (mAbs) and their derivatives have emerged as one of the most important classes of biotherapeutics in recent decades. The success of mAb is due to their high versatility, high target specificity, excellent clinical safety profile ...
Yang Zhang
doaj   +1 more source

Determination of the affinity constants for phage display albumin-binding peptides [PDF]

PeerJ, 2023
Background Phage display technology has been established as a powerful screening approach to select ligands or peptides for binding to proteins. Despite rapid growth in the field, there has been a relative dearth of quantitative criteria to measure the ...
Yi-Feng Shi
doaj   +2 more sources

Phage Display Informatics [PDF]

Computational and Mathematical Methods in Medicine, 2013
Phage display is an efficient laboratory technique that can be used to screen for specific peptides and proteins displayed on the surface of bacteriophage. Since Professor George Smith of the University of Missouri pioneered the powerful and flexible method in 1980s [1], it has been adapted and improved by many scientists from various fields.
Huang, Jian, Derda, Ratmir, Huang, Yanxin   +2 more
openaire   +2 more sources

SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology

mAbs, 2023
Phage display is an established method for the in vitro selection of recombinant antibodies and other proteins or peptides from gene libraries. Here we describe SpyDisplay, a phage display method in which the display is achieved via SpyTag/SpyCatcher ...
Sarah-Jane Kellmann, Christian Hentrich, Mateusz Putyrski, Hanh Hanuschka, Manuel Cavada, Achim Knappik, Francisco Ylera   +6 more
doaj   +1 more source

Advancement in the development of single chain antibodies using phage display technology [PDF]

PeerJ
Phage display technology has become an important research tool in biological research, fundamentally changing the traditional monoclonal antibody preparation process, and has been widely used in the establishment of antigen-antibody libraries, drug ...
Xiaohui Zheng, Qi Liu, Yimin Liang, Wenzhi Feng, Honghao Yu, Chunyu Tong, Bocui Song   +6 more
doaj   +2 more sources

The Biotechnological Application of Bacteriophages: What to Do and Where to Go in the Middle of the Post-Antibiotic Era

Microorganisms, 2023
Amid the escalating challenges of antibiotic resistance, bacterial infections have emerged as a global threat. Bacteriophages (phages), viral entities capable of selectively infecting bacteria, are gaining momentum as promising alternatives to ...
Su Jin Jo, Jun Kwon, Sang Guen Kim, Seung-Jun Lee   +3 more
doaj   +1 more source

Eliminating helper phage from phage display [PDF]

Nucleic Acids Research, 2006
Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using '
Chasteen, L., Ayriss, J., Pavlik, P., Bradbury, A. R. M.   +3 more
openaire   +2 more sources

Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA) [PDF]

, 2013
The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage
A Ghosh, AC Pawlyk, AM Quinn, CW Diehnelt, D Hessels, D Shen, DB Sodee, Duanwen Shen, E Basch, F Yi, Fei Xie, GA Lazar, H Liu, H Liu, H-S Lim, HD Agnew, IM Thompson, Irina U. Agoulnik, J Yu, JA Arter, JR Mesters, JR Newton, KA Noren, MI Davis, MI Milowsky, MJ Morris, MJ Morris, MJ Roobol, NH Bander, RK Nam, S Aggarwal, S Kim, S Minner, SE Lupold, SM Hillier, SR Banerjee, SR Banerjee, SR Banerjee, SR Banerjee, SS Chang, SZ Li, TJ Wigle, V Humblet, W. Barry Edwards, Y Chen, Y Chen, Y Chen, Z Xiao, ZY Zhu   +48 more
core   +11 more sources

Basics of Antibody Phage Display Technology

Toxins, 2018
Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display ...
Line Ledsgaard, Mogens Kilstrup, Aneesh Karatt-Vellatt, John McCafferty, Andreas H. Laustsen   +4 more
doaj   +1 more source

Oligopeptide M13 Phage Display in Pathogen Research

Viruses, 2013
Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the ...
Michael Hust, Torsten Meyer, Jonas Kügler, Jonas Zantow   +3 more
doaj   +1 more source