Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library [PDF]
, 2007 Background Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic phage and M13 filamentous phage and ...
Lauren RH Krumpe +4 more
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Deep Sequencing Analysis of Phage Libraries using Illumina Platform [PDF]
, 2014 This paper presents an analysis of phage-displayed libraries of peptides using Illumina. We describe steps for the preparation of short DNA fragments for deep sequencing and MatLab software for the analysis of the results.
Chu, Kiki +5 more
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Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy
Molecules, 2011 Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology.
Michael Hust +4 more
doaj +1 more source
Purification of phage display-modified bacteriophage T4 by affinity chromatography [PDF]
, 2011 Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz +6 more
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Nanodiscs allow phage display selection for ligands to non-linear epitopes on membrane proteins. [PDF]
PLoS ONE, 2013 In this work, we exploited a method that uses polytopic membrane proteins as targets for phage display selections. Membrane proteins represent the largest class of drug targets and drug discovery is mostly based on the identification of ligands binding ...
Marina Pavlidou +3 more
doaj +1 more source
The natural dietary genistein boosts bacteriophage-mediated cancer cell killing by improving phage-targeted tumor cell transduction [PDF]
, 2016 Gene therapy has long been regarded as a promising treatment for cancer. However, cancer gene therapy is still facing the challenge of targeting gene delivery vectors specifically to tumors when administered via clinically acceptable non-invasive ...
Albahrani, M +5 more
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Cycle Sequencing of Filamentous Phage DNA Using a Biotinylated Primer and ΔTaq DNA Polymerase
BioTechniques, 1997 We describe a rapid nonradioactive, double-stranded phage DNA sequencing method using ΔTaq DNA polymerase in cycle reaction for phage peptide-display library screening.
Jianping Qiu +3 more
doaj +1 more source
Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
Вавиловский журнал генетики и селекции, 2021 Combinatorial biology methods offer a good solution for targeting interactions of specific molecules by a high-throughput screening and are widely used for drug development, diagnostics, identification of novel monoclonal antibodies, search for linear ...
A. N. Chikaev +3 more
doaj +1 more source
Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: peach Pru p 3 allergen as a model. [PDF]
, 2008 Lipid transfer proteins (LTPs) are the major allergens of Rosaceae fruits in the Mediterranean area. Pru p 3, the LTP and major allergen of peach, is a suitable model for studying food allergy and amino acid sequences related with its IgE-binding ...
Compes, Esther +7 more
core +2 more sources
Direct evidence of ZnO morphology modification via the selective adsorption of ZnO-binding peptides [PDF]
, 2011 Biomolecule-mediated ZnO synthesis has great potential for the tailoring of ZnO morphology for specific application in biosensors, window materials for display and solar cells, dye-sensitized solar cells (DSSCs), biomedical materials, and photocatalysts ...
Aisawa +103 more
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