Results 21 to 30 of about 63,473 (252)
Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy
Molecules, 2011 Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology.
Michael Hust +4 more
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Molecules, 2021 Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins.
Wei Sun, Yan Zhang, Zhigang Ju
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Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
Вавиловский журнал генетики и селекции, 2021 Combinatorial biology methods offer a good solution for targeting interactions of specific molecules by a high-throughput screening and are widely used for drug development, diagnostics, identification of novel monoclonal antibodies, search for linear ...
A. N. Chikaev +3 more
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Cycle Sequencing of Filamentous Phage DNA Using a Biotinylated Primer and ΔTaq DNA Polymerase
BioTechniques, 1997 We describe a rapid nonradioactive, double-stranded phage DNA sequencing method using ΔTaq DNA polymerase in cycle reaction for phage peptide-display library screening.
Jianping Qiu +3 more
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Nanodiscs allow phage display selection for ligands to non-linear epitopes on membrane proteins. [PDF]
PLoS ONE, 2013 In this work, we exploited a method that uses polytopic membrane proteins as targets for phage display selections. Membrane proteins represent the largest class of drug targets and drug discovery is mostly based on the identification of ligands binding ...
Marina Pavlidou +3 more
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FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
FEBS Letters, EarlyView.Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley +1 more source
Phage display technology: applications and innovations
Genetics and Molecular Biology, 2005 The expression of exogenous peptides on the surface of filamentous bacteriophage was initially described by Smith in 1985. Since his first study, different molecules such as small peptides and antibodies have been displayed on coat proteins of phage ...
Marco Antonio Arap
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Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Frontiers in Microbiology, 2015 In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage
Katrina F Tjhung +4 more
doaj +1 more source