Results 41 to 50 of about 114,213 (298)
Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies. [PDF]
, 2018 Human cells express thousands of different surface proteins that can be used for cell classification, or to distinguish healthy and disease conditions.
Adams, Jarrett J +12 more
core +1 more source
Novel applications of shotgun phage display [PDF]
, 2004 In a shotgun phage display library, theoretically, the entire proteome of a bacterium is represented. Phages displaying specific polypeptides can be isolated by affinity selection, while the corresponding gene remains physically linked to the gene ...
Rosander, Anna
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FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source
Phage display technology: applications and innovations
Genetics and Molecular Biology, 2005 The expression of exogenous peptides on the surface of filamentous bacteriophage was initially described by Smith in 1985. Since his first study, different molecules such as small peptides and antibodies have been displayed on coat proteins of phage ...
Marco Antonio Arap
doaj +1 more source
Phage display selected magnetite interacting Adhirons for shape controlled nanoparticle synthesis [PDF]
, 2015 Adhirons are robust, well expressing, peptide display scaffold proteins, developed as an effective alternative to traditional antibody binding proteins for highly specific molecular recognition applications.
Bramble, Jonathan P. +8 more
core +2 more sources
HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein
FEBS Open Bio, EarlyView.Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley +1 more source
Frontiers in Microbiology, 2015 In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage
Katrina F Tjhung +4 more
doaj +1 more source
Isolation, characterisation and experimental evolution of phage that infect the horse chestnut tree pathogen, Pseudomonas syringae pv. aesculi [PDF]
, 2020 Bleeding canker of horse chestnut trees is a bacterial disease, caused by the bacterium Pseudomonas syringae pv. aesculi, estimated to be present in ~ 50% of UK horse chestnut trees.
Jackson, Robert W. +4 more
core +3 more sources
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Rapid, multiplexed microfluidic phage display [PDF]
, 2011 The development of a method for high-throughput, automated proteomic screening could impact areas ranging from fundamental molecular interactions to the discovery of novel disease markers and therapeutic targets.
Ahmad, Habib +6 more
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