Results 41 to 50 of about 73,738 (335)
Cycle Sequencing of Filamentous Phage DNA Using a Biotinylated Primer and ΔTaq DNA Polymerase
BioTechniques, 1997 We describe a rapid nonradioactive, double-stranded phage DNA sequencing method using ΔTaq DNA polymerase in cycle reaction for phage peptide-display library screening.
Jianping Qiu +3 more
doaj +1 more source
Nanodiscs allow phage display selection for ligands to non-linear epitopes on membrane proteins. [PDF]
PLoS ONE, 2013 In this work, we exploited a method that uses polytopic membrane proteins as targets for phage display selections. Membrane proteins represent the largest class of drug targets and drug discovery is mostly based on the identification of ligands binding ...
Marina Pavlidou +3 more
doaj +1 more source
High-resolution epitope mapping of anti-Hu and anti-Yo autoimmunity by programmable phage display
Brain Communications, 2020 Paraneoplastic neurological disorders are immune-mediated diseases understood to manifest as part of a misdirected anti-tumor immune response. Paraneoplastic neurological disorder-associated autoantibodies can assist with diagnosis and enhance our ...
B. O’Donovan +12 more
semanticscholar +1 more source
Construction of Synthetic Antibody Phage-Display Libraries
, 2017 Synthetic antibody libraries provide a vast resource of renewable antibody reagents that can rival or exceed those of natural antibodies and can be rapidly isolated through controlled in vitro selections.
Nilvebrant, Johan, +3 more
core +1 more source
Nature Communications, 2019 The filamentous bacteriophage fd bind a cell target with exquisite specificity through its few copies of display peptides, whereas nanoparticles functionalized with hundreds to thousands of synthetically generated phage display peptides exhibit variable ...
Lin-Ping Wu +7 more
semanticscholar +1 more source
Selection of functional human antibodies from retroviral display libraries
, 2004 Antibody library technology represents a powerful tool for the discovery and design of antibodies with high affinity and specificity for their targets.
Álvarez-Vallina, Luis +12 more
core +1 more source
Phage display as a tool for vaccine and immunotherapy development
Bioengineering & Translational Medicine, 2019 Bacteriophages, or phages, are viruses that specifically infect bacteria and coopt the cellular machinery to create more phage proteins, eventually resulting in the release of new phage particles.
Krystina L Hess, C. Jewell
semanticscholar +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Modulation of antibody display on M13 filamentous phage
, 1998 Here we describe a phage vector for the display of single chain antibodies and polypeptides on the surface of filamentous M13 phage which permits facile manipulation of the valency of display.
Kang, A.S., He, M., Chappel, J.A.
core +1 more source
FEBS Letters, EarlyView.Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley +1 more source