Results 51 to 60 of about 978,488 (287)
Molecules, 2011 Since the initial description of phage display technology for the generation of human antibodies, a variety of selection methods has been developed. The most critical parameter for all in vitro-based approaches is the quality of the antibody library ...
Kathrin Tissot +3 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The development of immunoassays for the detection of bovine brucellosis and aflatoxin B1 [PDF]
, 2004 The research discussed in this thesis focuses on the development and characterisation of immunoassays for the detection of aflatoxin Bi (AFBi), a toxic fungal metabolite, and for the diagnosis of bovine brucellosis, a highly contagious disease of cattle ...
Dunne, Lynsey
core +2 more sources
Phage Therapy: Past، Present and Future [PDF]
Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī YazdIntroduction: As viral agents، phages serve a significant purpose in molecular biotechnology. These viruses are considered as ideal tools in this field and recent studies revealed their wide potential in biomedicine.
Farzaneh Fathi, Mehdi Zeinoddini
doaj
High-throughput profiling of posttranslational modification enzymes by phage display
BioTechniques, 2007 Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome.
Michelle Yen, Jun Yin
doaj +1 more source
Phage Display-Based Nanotechnology Applications in Cancer Immunotherapy
Molecules, 2020 Phage display is a nanotechnology with limitless potential, first developed in 1985 and still awaiting to reach its peak. Awarded in 2018 with the Nobel Prize for Chemistry, the method allows the isolation of high-affinity ligands for diverse substrates,
Martina Goracci +2 more
doaj +1 more source
Eliminating helper phage from phage display [PDF]
Nucleic Acids Research, 2006 Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using '
Chasteen, L. +3 more
openaire +2 more sources
Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: peach Pru p 3 allergen as a model. [PDF]
, 2008 Lipid transfer proteins (LTPs) are the major allergens of Rosaceae fruits in the Mediterranean area. Pru p 3, the LTP and major allergen of peach, is a suitable model for studying food allergy and amino acid sequences related with its IgE-binding ...
Tordesillas Villuendas, Leticia +7 more
core +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display
Frontiers in Microbiology, 2016 Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in
Dragana eGagic +7 more
doaj +1 more source