Results 51 to 60 of about 73,738 (335)

Phage display technology: applications and innovations

Genetics and Molecular Biology, 2005
The expression of exogenous peptides on the surface of filamentous bacteriophage was initially described by Smith in 1985. Since his first study, different molecules such as small peptides and antibodies have been displayed on coat proteins of phage ...
Marco Antonio Arap
doaj   +1 more source

Selection of gonadotrophin surge attenuating factor phage antibodies by bioassay [PDF]

, 2005
Peer ...
Fowler, Paul Alfred Francois, Sorsa-Leslie, Tarja Kristina, Paul A Fowler, Mason, H D, Sorsa-Leslie Tarja, Mason, HD, Mason Helen D, Harris, WJ, Fowler, PA, Sorsa-Leslie, T, Tarja Sorsa-Leslie, Helen D Mason, Harris William J, Harris, William Joseph, Fowler Paul A, William J Harris   +15 more
core   +1 more source

Regularized indirect learning improves phage display ligand discovery

, 2023
Phage display is commonly employed for the discovery of high affinity ligands to biomolecular targets. However, ranking the discovered ligands for their affinity and specificity to the target is obscured by genetic amplification bias and amplification of
Michael A., Lee, Bradley L., Pentelute, Andrei, Loas, Jeffrey Y.-K., Wong, Yitong, Tseo, Yehlin, Cho, Ratmir, Derda, Joseph S., Brown, Soojung, Yang, Chae Rin, Kim, Rafael, Gomez-Bombarelli   +10 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Intra-domain phage display (ID-PhD) of peptides and protein mini-domains censored from canonical pIII phage display

Frontiers in Microbiology, 2015
In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage
Katrina F Tjhung, Frédérique eDeiss, Jessica eTran, Ying eChou, Ratmir eDerda   +4 more
doaj   +1 more source

Automated phage display on challenging targets

, 2023
reservedAl giorno d’oggi, il settore farmaceutico si ritrova ad affrontare diverse sfide – una in particolare riguarda la complessità nello sviluppare nuove molecole in grado di legare bersagli più complicati, cosiddetti “undruggable”.
RAMPINI, MARTA
core  

Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF [PDF]

, 2008
Background To develop antibacterial agents having novel modes of action against bacterial cell wall biosynthesis, we targeted the essential MurF enzyme of the antibiotic resistant pathogen Pseudomonas aeruginosa.
Sanschagrin, François, Tom Clarke, Catherine Paradis-Bleau, Blewett, Ann, Roger C Levesque, Clark, Tom, Paradis-Bleau , Catherine, Ann Blewett, Adrian Lloyd, Levesque, Roger C., Lloyd, Adrian, François Sanschagrin, Timothy DH Bugg, Bugg, Tim   +13 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

An amber obligate active site-directed ligand evolution technique for phage display

Nature Communications, 2020
Although noncanonical amino acids (ncAAs) were first incorporated into phage libraries through amber suppression nearly two decades ago, their application for use in drug discovery has been limited due to inherent library bias towards sense-containing ...
Jeffery M. Tharp, J. T. Hampton, C. A. Reed, Andreas Ehnbom, P. Chen, Jared S. Morse, Yadagirri Kurra, L. M. Pérez, Shiqing Xu, W. Liu   +9 more
semanticscholar   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source