Frameshifting in the P6 cDNA Phage Display System
Molecules, 2010 Phage display is a powerful technique that enables easy identification of targets for any type of ligand. Targets are displayed at the phage surface as a fusion protein to one of the phage coat proteins.
Veerle Somers +3 more
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Purification of phage display-modified bacteriophage T4 by affinity chromatography [PDF]
, 2011 Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz +6 more
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Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy
Molecules, 2011 Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology.
Michael Hust +4 more
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Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library [PDF]
, 2007 Background Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic phage and M13 filamentous phage and ...
Lauren RH Krumpe +4 more
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Nanodiscs allow phage display selection for ligands to non-linear epitopes on membrane proteins. [PDF]
PLoS ONE, 2013 In this work, we exploited a method that uses polytopic membrane proteins as targets for phage display selections. Membrane proteins represent the largest class of drug targets and drug discovery is mostly based on the identification of ligands binding ...
Marina Pavlidou +3 more
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Theranostic applications of phage display to control leishmaniasis: Selection of biomarkers for serodiagnostics, vaccination, and immunotherapy [PDF]
, 2017 © 2015, Sociedade Brasileira de Medicina Tropical. All rights reserved. Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries.
Chávez-Fumagalli, Miguel A. +5 more
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Cycle Sequencing of Filamentous Phage DNA Using a Biotinylated Primer and ΔTaq DNA Polymerase
BioTechniques, 1997 We describe a rapid nonradioactive, double-stranded phage DNA sequencing method using ΔTaq DNA polymerase in cycle reaction for phage peptide-display library screening.
Jianping Qiu +3 more
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Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
Вавиловский журнал генетики и селекции, 2021 Combinatorial biology methods offer a good solution for targeting interactions of specific molecules by a high-throughput screening and are widely used for drug development, diagnostics, identification of novel monoclonal antibodies, search for linear ...
A. N. Chikaev +3 more
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The natural dietary genistein boosts bacteriophage-mediated cancer cell killing by improving phage-targeted tumor cell transduction [PDF]
, 2016 Gene therapy has long been regarded as a promising treatment for cancer. However, cancer gene therapy is still facing the challenge of targeting gene delivery vectors specifically to tumors when administered via clinically acceptable non-invasive ...
Albahrani, M +5 more
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Phage-displayed peptide libraries [PDF]
Current Opinion in Biotechnology, 1998 Over the past year, significant advances have been achieved through the use of phage-displayed peptide libraries. A wide variety of bioactive molecules, including antibodies, receptors and enzymes, have selected high-affinity and/or highly-specific peptide ligands from a number of different types of peptide library.
M B, Zwick, J, Shen, J K, Scott
openaire +2 more sources