Results 11 to 20 of about 195,175 (290)

Recognizing Phosphatidylinositol 3-Phosphate [PDF]

Cell, 2001
Phosphatidylinositol 3-phosphate directs the endosomal localization of regulatory proteins by binding to FYVE and PX domains. New structures of these domains complexed with the phosphoinositide headgroup show how interactions with phosphate and hydroxyl groups differentiate this lipid from all others.
Misra, Saurav, Miller, Gregory J, Hurley, James H   +2 more
openaire   +4 more sources

Cyclodextrins enhance recombinant phosphatidylinositol phosphate kinase activity [PDF]

Journal of Lipid Research, 2004
Inositol lipid kinases have been studied extensively in both plant and animal systems. However, major limitations for in vitro studies of recombinant lipid kinases are the low specific activity and instability of the purified proteins.
Amanda J. Davis, Imara Y. Perera, Wendy F. Boss   +2 more
doaj   +3 more sources

Exploring phosphatidylinositol 5-phosphate 4-kinase function

Advances in Biological Regulation, 2015
The family of phosphatidylinositol 5-phosphate 4-kinases (PI5P4Ks) is emerging from a comparative backwater in inositide signalling into the mainstream, as is their substrate, phosphatidylinositol 5-phosphate (PI5P). Here we review some of the key questions about the PI5P4Ks, their localisation, interaction, and regulation and also we summarise our ...
Bulley, Simon J, Clarke, Jonathan H, Droubi, Alaa, Giudici, Maria-Luisa, Irvine, Robin F   +4 more
openaire   +7 more sources

Structural basis for phosphatidylinositol-phosphate biosynthesis. [PDF]

Nat Commun, 2015
AbstractPhosphatidylinositol is critical for intracellular signalling and anchoring of carbohydrates and proteins to outer cellular membranes. The defining step in phosphatidylinositol biosynthesis is catalysed by CDP-alcohol phosphotransferases, transmembrane enzymes that use CDP-diacylglycerol as donor substrate for this reaction, and either inositol
Clarke OB, Tomasek D, Jorge CD, Dufrisne MB, Kim M, Banerjee S, Rajashankar KR, Shapiro L, Hendrickson WA, Santos H, Mancia F.   +10 more
europepmc   +5 more sources

Differentiating human phospholipase A₂'s activity toward phosphatidylinositol, phosphatidylinositol phosphate and phosphatidylinositol bisphosphate. [PDF]

Biochim Biophys Acta Mol Cell Biol Lipids
Phospholipase A2's (PLA2's) constitute a superfamily of enzymes that hydrolyze the sn-2 fatty acyl chain on glycerophospholipids. We have previously reported that each PLA2 Type shows a unique substrate specificity for the molecular species it hydrolyzes, especially the acyl chain that is cleaved from the sn-2 position and to some extent the polar ...
Hayashi D, Dennis EA.
europepmc   +4 more sources

Phosphatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate regulate phagolysosome biogenesis [PDF]

Proceedings of the National Academy of Sciences, 2015
Significance Phagocytes are key to innate immunity, as they ingest, kill, and degrade foreign particles, such as microbes. Particle uptake results in the formation of phagosomes that eventually fuse with lysosomes, the main degradative compartments in cells.
Jeschke, Andreas, Zehethofer, Nicole, Lindner, Buko, Krupp, Jessica, Schwudke, Dominik, Haneburger, Ina, Jovic, Marko, Backer, Jonathan M, Balla, Tamas, Hilbi, Hubert, Haas, Albert   +10 more
openaire   +3 more sources

PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase

Life Science Alliance, 2023
PIP4K is a lipid kinase implicated in tumour growth, immune function, and metabolic regulation. This study shows that PIP4K regulates PI3P levels and autophagy, thereby controlling cell size in Drosophila salivary glands. Phosphatidylinositol 3-phosphate
Avishek Ghosh, Aishwarya Venugopal, Dhananjay Shinde, Sanjeev Sharma, Meera Krishnan, Swarna Mathre, Harini Krishnan, Sankhanil Saha, Padinjat Raghu   +8 more
doaj   +1 more source

Formation of phosphatidylinositol 3-phosphate by isomerization from phosphatidylinositol 4-phosphate. [PDF]

Proceedings of the National Academy of Sciences, 1991
We have synthesized phosphatidylinositol 3-phosphate from phosphatidylinositol 4-phosphate by using diisopropylcarbodiimide to promote migration of the 4-phosphate via a cyclic phosphodiester intermediate. The product was isolated by a thin-layer chromatographic method that depends on the ability of phosphatidylinositol 4-phosphate, but not ...
J P, Walsh, K K, Caldwell, P W, Majerus
openaire   +2 more sources

Plasma Membrane Phosphatidylinositol 4-Phosphate Is Necessary for Virulence of Candida albicans

mBio, 2022
Phosphatidylinositol lipids regulate key processes, including vesicle trafficking and cell polarity. A recent study identified novel roles for phosphatidylinositol 4-phosphate (PI4P) in the plasma membrane of the fungal pathogen Candida albicans ...
James B. Konopka
doaj   +1 more source

Nuclear inositides

European Journal of Histochemistry, 2009
DAG, 1,2-diacylglycerol; EGF, epidermal growth factor; TNF, tumour necrosis factor; IGF-I, insulinlike growth factor I; Ins(n)P, inositol phosphate; Ins(n,n)P2, inositol bisphosphate; Ins(n,n,n)P3, inositol trisphosphate; MEL, murine erythroleukemia ...
C D'Santos, JH Clarke, M Roefs, JR Halstead, N Divecha   +4 more
doaj   +3 more sources