Results 221 to 230 of about 837,784 (254)

The phosphatidylinositol 4-phosphate 5-kinase family

Advances in Enzyme Regulation, 1996
The existence of a PIP5K family of enzymes has been suggested by Western blotting and purification of numerous PIP5Ks from various tissues and cell types. The erythrocyte has at least two PIP5Ks, named PIP5KI and PIP5KII, while the brain appears to have even more isoforms.
J C, Loijens, I V, Boronenkov, G J, Parker, R A, Anderson   +3 more
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Phosphatidylinositol 4-Kinase and Phosphatidylinositol 4-Phosphate 5-Kinase Assays

2013
Inositol lipid kinases are perhaps the easiest and most straightforward enzymes in the phosphoinositide pathway to analyze. In addition to monitoring lipid kinase-specific activity, lipid kinase assays can be used to quantify the inositol lipids present in isolated membranes (Jones et al., Methods Mol Biol 462:75-88, 2009).
Yang Ju, Im, Irena, Brglez, Catherine, Dieck, Imara Y, Perera, Wendy F, Boss   +4 more
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Intracellular trafficking and turnover of phosphatidylinositol 3-phosphate

Seminars in Cell & Developmental Biology, 2001
Phosphatidylinositol 3-kinases (PI 3-kinases) regulate cellular functions through the 3'-phosphorylation of phosphatidylinositol (PI) and its derivatives. The PI 3-kinase product phosphatidylinositol 3-phosphate [PI(3)P] functions to recruit and activate effector proteins containing FYVE zinc finger domains.
H, Stenmark, D J, Gillooly
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Enzymatic synthesis and hydrolysis of [32P]phosphatidylinositol phosphate

Biochemical and Biophysical Research Communications, 1985
Phosphatidylinositol kinase activity in plasma membrane preparations of mouse liver was found to be comparable to that in A431 cells and higher than that in three human tumor xenografts. This activity was exploited in preparing 32P-labeled phosphatidylinositol phosphate of high specific radioactivity in which approximately 4% of the radioactivity of ...
A F, Knowles, C M, Lawrence
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Inhibition of phosphatidylinositol-4-phosphate kinase by its product phosphatidylinositol-4,5-bisphosphate

Biochemical and Biophysical Research Communications, 1985
Phosphatidylinositol 4,5-bisphosphate (PIP2) is enzymatically produced when high speed supernatant fraction from bovine retina is incubated with [gamma-32P]ATP and phosphatidylinositol 4-phosphate (PIP) as substrates. Exogenously added PIP2 inhibits PIP kinase activity 50% at equimolar concentrations of product and substrate.
L A, Van Rooijen, M, Rossowska, N G, Bazan   +2 more
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Review of Phosphatidylinositol Phosphate Kinase in Phosphatidylinositol Signaling Pathway

2014
Phosphatidylinositol signaling pathway is an important signal transduction pathway that plays critical roles in multiple cell processes first demonstrated in animal cells. Research evidences have shown that although not exactly the same as it is in yeast and mammalian cells, phosphatidylinositol signaling pathway also exists in plant cells and is ...
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Differential effects of spermine on phosphatidylinositol 3-kinase and phosphatidylinositol phosphate 5-kinase

Life Sciences, 1995
The metabolism of phosphoinositides plays an important role in the signal transduction pathways. We report here that naturally occurring polyamines affect the activities of phosphatidylinositol (PI) 3-kinase and PI 4-phosphate (PIP) 5-kinase differently.
S S, Singh, A, Chauhan, H, Brockerhoff, V P, Chauhan   +3 more
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Nuclear Phosphatidylinositol Signaling: Focus on Phosphatidylinositol Phosphate Kinases and Phospholipases C.

Journal of cellular physiology, 2016
Phosphatidylinositol (PI) metabolism represents the core of a network of signaling pathways which modulate many cellular functions including cell proliferation, cell differentiation, apoptosis, and membrane trafficking. An array of kinases, phosphatases, and lipases acts on PI creating an important number of second messengers involved in different ...
POLI, ALESSANDRO, BILLI, ANNA MARIA, MONGIORGI, SARA, RATTI, STEFANO, Mccubrey, James A., Suh, Pann Ghill, COCCO, LUCIO ILDEBRANDO, RAMAZZOTTI, GIULIA   +7 more
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Quantification of Phosphatidylinositol Phosphate Species in Purified Membranes

2017
Phosphoinositide lipids (PIPs) are required for various processes during macroautophagy, such as phagophore formation and autophagosome-lysosome fusion. Hence, quantification of the seven PIP species in autophagosome membranes is an important tool to understand how these lipids govern the transition of autophagosomes into autolysosomes.
A, Jeschke, N, Zehethofer, D, Schwudke, A, Haas   +3 more
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Spatiotemporal analysis of phosphatidylinositol 4-phosphate dynamics

Phosphatidylinositol 4-phosphate (PI4P) is a fundamental phosphoinositide that controls a variety of cellular processes, including signaling, membrane trafficking and intracellular lipid transport. Given the importance of PI4P, tools and methods for monitoring its dynamics are in high demand.
Asami Kawasaki, Shinya Tsukiji, Fubito Nakatsu   +2 more
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