Results 61 to 70 of about 18,478 (212)

Rapid Shotgun Phosphoproteomics Analysis

, 2021
In this chapter, we describe a rapid workflow for the shotgun global phosphoproteomics analysis. The strategy is based on the use of accelerated in-solution trypsin digestion under an ultrasonic field by high-intensity focused ultrasound (HIFU) coupled to titanium dioxide (TiO2) selective phosphopeptide enrichment, fractionation by strong cation ...
Carrera, Mónica, Cañas, Benito, López-Ferrer, Daniel   +2 more
openaire   +3 more sources

Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway

Frontiers in Oncology, 2022
Intrahepatic cholangiocarcinoma (ICC) is a malignant tumor that originates from bile duct’s epithelial cells and is usually characterized by insidious symptoms and poor prognosis.
Zhili Xia, Minzhen Li, Meng Hu, Yanyan Lin, Yanyan Lin, Lawrence Lawer Atteh, Wenkang Fu, Long Gao, Mingzhen Bai, Chongfei Huang, Ping Yue, Ping Yue, Yu Liu, Wenbo Meng, Wenbo Meng, Wenbo Meng   +15 more
doaj   +1 more source

The dynamic organization of fungal acetyl-CoA carboxylase [PDF]

, 2016
Acetyl-CoA carboxylases (ACCs) catalyse the committed step in fatty-acid biosynthesis: the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA. They are important regulatory hubs for metabolic control and relevant drug targets for the treatment of ...
Hagmann, Anna, Hunkeler, Moritz, Imseng, Stefan, Maier, Timm, Stuttfeld, Edward   +4 more
core   +1 more source

Evolutionary patterns of phosphorylated serines [PDF]

, 2011
Posttranslationally modified amino acids are chemically distinct types of amino acids and in terms of evolution they might behave differently from their non-modified counterparts.
Yerbol Z Kurmangaliyev, Alexander Goland, Mikhail S Gelfand   +2 more
core   +2 more sources

The Multifaceted Role of Rad9 in the DNA Damage Response of Saccharomyces cerevisiae

Yeast, EarlyView.
ABSTRACT To maintain the integrity of the genome, cells have evolved a complex signalling system, termed the DNA damage response (DDR), which detects DNA damage and promotes DNA repair. To date, over 600 proteins have been identified that play an integral role in the DDR. RAD9, encoding a DDR mediator protein, was the prototypical DNA damage checkpoint
A. Kiely, F. O'Halloran, P. Young, N. F. Lowndes, M. Grenon, K. Finn   +5 more
wiley   +1 more source

Phosphoproteomics

, 2012
The book provides an overview of state-of-art techniques for the purification, analysis and quantification of proteins in complex samples using different enrichment strategies.
F. Lonardoni, BOSSI, Alessandra Maria
openaire   +3 more sources

Assessment of Protein Content and Phosphorylation Level in Synechocystis sp. PCC 6803 under Various Growth Conditions Using Quantitative Phosphoproteomic Analysis

Molecules, 2020
The photosynthetic apparatus and metabolic enzymes of cyanobacteria are subject to various controls, such as transcriptional regulation and post-translational modifications, to ensure that the entire cellular system functions optimally.
Masakazu Toyoshima, Yuma Tokumaru, Fumio Matsuda, Hiroshi Shimizu   +3 more
doaj   +1 more source

Neurotransmitters Drive Combinatorial Multistate Postsynaptic Density Networks [PDF]

, 2009
The mammalian postsynaptic density (PSD) comprises a complex collection of ~1100 proteins. Despite extensive knowledge of individual proteins, the overall organization of the PSD is poorly understood.
Choudhary, Jyoti S, Coba, Marcelo P, Collins, Mark O, Croning, Mike D R, Grant, Seth G N, Kopanitsa, Maksym V, Pocklington, Andrew J, Swamy, Sajani, Uren, Rachel T   +8 more
core   +1 more source

PDE4D and PDE3B orchestrate distinct cAMP microdomains in 3T3‐L1 adipocytes

British Journal of Pharmacology, EarlyView.
Basal conditions: •Ins/PDE3B lowers cytoplasmic cAMP (cyt‐cAMP) without affecting plasma membrane cAMP (pm‐cAMP). •Insulin decreases lipid droplet cAMP (LD‐cAMP) independent of PDE3B. •FGF1/PDE4D modestly reduces both cyt‐ and pm‐cAMP, while PDE4D alone can modulate LD‐cAMP. ISO stimulation: •Ins/PDE3B has minimal impact on cyt‐cAMP.
Johannes Krier, David Spähn, David Arturo Juarez Lopez, Judith L. Nono, Judith Seigner, Lisa Jacob, Siegfried Ussar, Robert Lukowski, Andreas L. Birkenfeld, Gencer Sancar   +9 more
wiley   +1 more source

Chilling stress response in tobacco seedlings: insights from transcriptome, proteome, and phosphoproteome analyses

Frontiers in Plant Science
Tobacco (Nicotiana tabacum L.) is an important industrial crop, which is sensitive to chilling stress. Tobacco seedlings that have been subjected to chilling stress readily flower early, which seriously affects the yield and quality of their leaves ...
Xiuhong Shao, Zhenchen Zhang, Faheng Yang, Yongchao Yu, Junjie Guo, Jiqin Li, Tingyu Xu, Xiaoying Pan   +7 more
doaj   +1 more source