Citrobacter amalonaticus phytase on the cell surface of Pichia pastoris exhibits high pH stability as a promising potential feed supplement. [PDF]
Phytase expressed and anchored on the cell surface of Pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. Furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst.
Cheng Li +4 more
doaj +1 more source
Safety and efficacy profile of a phytase produced by fermentation and used as a feed additive
Enzymes can aid in optimal feed stock utilization when used as feed additives. A range of toxicological studies were performed to evaluate the safety profile of a novel phytase (phytase HM) from Citrobacter braakii produced in Aspergillus oryzae. Phytase
Michael Thorsen +5 more
doaj +1 more source
Potential immune-modulatory effects of wheat phytase on the performance of a mouse macrophage cell line, Raw 264.7, exposed to long-chain inorganic polyphosphate [PDF]
Objective This experiment was conducted to find out the immunological effects of wheat phytase when long-chain inorganic polyphosphate (polyP) treated with wheat phytase was added to a macrophage cell line, Raw 264.7, when compared to intact long-chain ...
Jeongmin An, Jaiesoon Cho
doaj +1 more source
Cloning of Bacillus subtilis phytase gene construct in Escherichia coli
Background and Objectives: Phytase has a hydrolysis function of phytic acid, which yields inorganic phosphate. Bacillus species can produce thermostable alkaline phytase.
Mahdiyar Iravani Saadi +6 more
doaj +1 more source
Wheat phytase can alleviate the cellular toxic and inflammatory effects of lipopolysaccharide
The objective of this study was to characterize the enzymatic hydrolysis of lipopolysaccharide (LPS) by wheat phytase and to investigate the effects of wheat phytase-treated LPS on in vitro toxicity, cell ...
Jeongmin An, Jaiesoon Cho
doaj +1 more source
Effects of simultaneous supplementation of 3-phytase and 6-phytase on phosphorus and calcium digestibility in growing pigs [PDF]
Objective The present study aimed to determine the effects of supplemental 3-phytase, 6-phytase, or both on total tract digestibility of phosphorus (P) and calcium in corn-soybean meal-based diets fed to growing pigs.
Hyunseok Do, Beob Gyun Kim
doaj +1 more source
Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis [PDF]
Phytase has a hydrolysis function on phytic acid which yields inorganic phosphate. Using phytase enzymes in domestic animals have a positive effect on optimal diet intake. Bacillus species can only produce thermostable alkaline phytase.
Hamid Arian nejad +6 more
doaj +1 more source
Phytase produced from an edible local mushroom P.ostreatus11L was purified in three steps, in precipitation with ammonium Sulfate (saturation ratio70%), the specific activity of phytase increased from 0.38u/mg protein in crude extract to 0.77 u/mg ...
Abdullah A. Hassan, HawazinA.Al-Jobory
doaj +1 more source
Endogenous enzyme activities and tibia bone development of broiler chickens fed wheat-based diets supplemented with xylanase, β-glucanase and phytase [PDF]
Objective This study assessed the effect of different levels of xylanase, β-glucanase and phytase on intestinal enzyme activities and tibia bone development in broiler chickens fed wheat-based diets.
Mohammed Al-Qahtani +6 more
doaj +1 more source
Cultivation conditions for phytase production from recombinant escherichia coli DH5α [PDF]
Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α.
Mohd Ariff, Rafidah +6 more
core +1 more source

