PI(4,5)P2-dependent and -independent roles of PI4P in the control of hormone secretion by pituitary cells [PDF]
Frontiers in Endocrinology, 2023 Plasma membrane and organelle membranes are home to seven phosphoinositides, an important class of low-abundance anionic signaling lipids that contribute to cellular functions by recruiting cytoplasmic proteins or interacting with the cytoplasmic domains
Stanko S. Stojilkovic, Tamas Balla
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Investigation of HIV-1 Gag binding with RNAs and lipids using Atomic Force Microscopy. [PDF]
PLoS ONE, 2020 Atomic Force Microscopy was utilized to study the morphology of Gag, ΨRNA, and their binding complexes with lipids in a solution environment with 0.1Å vertical and 1nm lateral resolution. TARpolyA RNA was used as a RNA control. The lipid used was phospha-
Shaolong Chen +6 more
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PI(4,5)P2 alleviates colitis by inhibiting intestinal epithelial cell pyroptosis through NNMT-mediated RBP4 m6A modification [PDF]
Cell Death and DiseaseLipid metabolism disorder is a critical feature of Crohn’s disease (CD). Phosphatidylinositol (PI) and its derivative, phosphatidylinositol bisphosphate (PIP2), are associated with CD. The mechanisms underlying such association remain unknown.
Qingfan Yang +10 more
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PI(4,5)P2 Imaging Using a GFP Reporter in Living Cells [PDF]
Bio-ProtocolPhosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is a phospholipid enriched on the cytoplasmic leaflet of the plasma membrane, where it plays important roles in membrane trafficking and cytoskeletal dynamics through proteins that directly bind to it. PI(
Mariam Alkandari +2 more
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Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy [PDF]
eLifeAnionic lipid molecules, including phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), are implicated in the regulation of epidermal growth factor receptor (EGFR).
Mitsuhiro Abe +4 more
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Two cooperative binding sites sensitize PI(4,5)P2 recognition by the tubby domain. [PDF]
Sci Adv, 2022 Phosphoinositides (PIs) are lipid signaling molecules that operate by recruiting proteins to cellular membranes via PI recognition domains. The dominant PI of the plasma membrane is phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].
Thallmair V +5 more
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PI(4,5)P2-dependent regulation of endothelial tip cell specification contributes to angiogenesis. [PDF]
Sci Adv, 2023 Dynamic positioning of endothelial tip and stalk cells, via the interplay between VEGFR2 and NOTCH signaling, is essential for angiogenesis. VEGFR2 activates PI3K, which phosphorylates PI(4,5)P2 to PI(3,4,5)P3, activating AKT; however, PI3K/AKT does not ...
Davies EM +7 more
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Cholesterol promotes clustering of PI(4,5)P2 driving unconventional secretion of FGF2. [PDF]
J Cell Biol, 2022 FGF2 is a cell survival factor involved in tumor-induced angiogenesis that is secreted through an unconventional secretory pathway based upon direct protein translocation across the plasma membrane. Here, we demonstrate that both PI(4,5)P2-dependent FGF2
Lolicato F +15 more
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The Arabidopsis SAC9 enzyme is enriched in a cortical population of early endosomes and restricts PI(4,5)P2 at the plasma membrane [PDF]
eLife, 2022 Membrane lipids, and especially phosphoinositides, are differentially enriched within the eukaryotic endomembrane system. This generates a landmark code by modulating the properties of each membrane.
Alexis Lebecq +14 more
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Suppression of cell migration by phospholipase C-related catalytically inactive protein-dependent modulation of PI3K signalling [PDF]
Scientific Reports, 2017 The metabolic processes of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] into PI(3,4,5)P3 and the subsequent PI(3,4,5)P3 signalling are involved in cell migration.
Satoshi Asano +7 more
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