Results 21 to 30 of about 707,375 (141)
eLife, 2022 Synaptic communication relies on the fusion of synaptic vesicles with the plasma membrane, which leads to neurotransmitter release. This exocytosis is triggered by brief and local elevations of intracellular Ca2+ with remarkably high sensitivity.
Janus RL Kobbersmed +4 more
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Regulation of the leading edge motility by PI (4,5)P2-dependent lipid microdomains [PDF]
, 2005 The lipid second messenger PI(4,5)P2 modulates actin dynamics, and its local accumulation at plasmalemmal microdomains (rafts) might mediate regulation of protrusive motility.
Golub, Tamara
core +1 more source
Ca2+ induces PI(4,5)P2 clusters on lipid bilayers at physiological PI(4,5)P2 and Ca2+ concentrations [PDF]
, 2014 Calcium has been shown to induce clustering of PI(4,5)P2 at high and non-physiological concentrations of both the divalent ion and the phosphatidylinositol, or on supported lipid monolayers. In lipid bilayers at physiological conditions, clusters are not
Prieto, Manuel +4 more
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eLife, 2022 Membrane lipids, and especially phosphoinositides, are differentially enriched within the eukaryotic endomembrane system. This generates a landmark code by modulating the properties of each membrane.
Alexis Lebecq +14 more
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Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine
Journal of Lipid Research, 2006 Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a key role in the modulation of actin polymerization and vesicle trafficking. These processes seem to depend on the enrichment of PI(4,5)P2 in plasma membrane domains. Here, we show that PI(4,5)P2
Faébio Fernandes +3 more
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Cell Reports, 2017 Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) signaling is transient and spatially confined in live cells. How this pattern of signaling regulates transmitter release and hormone secretion has not been addressed. We devised an optogenetic approach to
Chen Ji, Fan Fan, Xuelin Lou
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PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism
Cell Reports, 2019 Summary: Insulin stimulates the conversion of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) to phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3), which mediates downstream cellular responses.
Diana G. Wang +14 more
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New Experimental Trends for Phosphoinositides Research on Ion Transporter/Channel Regulation
Journal of Pharmacological Sciences, 2014 .: Phosphoinositides(4,5)-bisphosphates [PI(4,5)P2] critically controls membrane excitability, the disruption of which leads to pathophysiological states.
Masayuki X. Mori, Ryuji Inoue
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Optogenetic dephosphorylation of phosphatidylinositol 4,5 bisphosphate in Xenopus laevis oocytes
STAR Protocols, 2023 Summary: Here, we present a protocol for optogenetic dephosphorylation of the phosphoinositide PI(4,5)P2 at the plasma membrane of Xenopus laevis oocytes. We first describe the co-injection of oocytes with cRNAs encoding (1) a light-activated PI(4,5)P2 5-
Kirin D. Gada +6 more
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Synchronized HIV assembly by tunable PIP2 changes reveals PIP2 requirement for stable Gag anchoring
eLife, 2017 HIV-1 assembles at the plasma membrane (PM) of infected cells. PM association of the main structural protein Gag depends on its myristoylated MA domain and PM PI(4,5)P2. Using a novel chemical biology tool that allows rapidly tunable manipulation of PI(4,
Frauke Mücksch +4 more
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