Results 21 to 30 of about 2,539 (164)

Protocol for HeLa Cells Infection with Escherichia coli Strains Producing Colibactin and Quantification of the Induced DNA-damage

Bio-Protocol, 2017
Strains of Escherichia coli bearing the pks genomic island synthesize the genotoxin colibactin. Exposure of eukaryotic cells to E. coli producing colibactin induces DNA damages, ultimately leading to cell cycle arrest, senescence and death.
Nadège Bossuet-Greif, Marcy Belloy, Michèle Boury, Eric Oswald, Jean-Philippe Nougayrede   +4 more
doaj   +2 more sources

Role of the pks genomic island in the synthesis of siderophores-microcins in Escherichia coli

, 2019
Siderophore-microcins H47 and M (MccH47 and MccM) are antimicrobial peptides, built from a peptidic precursor modified with a siderophore enterobactin derived moiety. They are responsible for Escherichia coli strain Nissle (EcN) antagonistic activity against pathogenic enterobacteria.
Massip, Clémence
openaire   +2 more sources

The pks genomic island of Escherichia coli : structure-function of the ClbP protein and epidemiological studies

, 2011
The pks genomic island of Escherichia coli and other Enterobacteriaceae encodes polyketide andnonribosomal peptide synthases that build a putative hybrid PK-NRP compound. This compound designatedColibactin induces DNA double-strand breaks in eukaryotic cells.The pks-encoded enzymatic machinery comprises an essential protein ClbP, atypical for this type
Dubois, Damien
openaire   +2 more sources

Deciphering the biosynthetic pathways of lichen acids. [PDF]

New Phytol
Summary Depsides and depsidones are polyketide‐derived lichen acids widely distributed in lichen thalli, yet the biosynthetic gene clusters (BGCs) responsible for their production remain poorly understood. To address this gap, we investigated the diversity and evolutionary relationships of polyketide BGCs in lichens.
Kim W, Shishido TK, Pham HT, Kang KB, Liu R, Wahlsten M, Collemare J, Park H, Oh SO, Ha HH, Kim H, Hur JS.   +11 more
europepmc   +2 more sources

Evolutionary Dynamics Based on Comparative Genomics of Pathogenic Escherichia coli Lineages Harboring Polyketide Synthase (pks) Island. [PDF]

mBio, 2021
Extraintestinal pathologies caused by highly virulent strains of E. coli amount to clinical implications with high morbidity and mortality rates. Pathogenic E. coli strains are evolving with the horizontal acquisition of mobile genetic elements, including pathogenicity islands such as the
Suresh A, Shaik S, Baddam R, Ranjan A, Qumar S, Jadhav S, Semmler T, Ghazi IA, Wieler LH, Ahmed N.   +9 more
europepmc   +6 more sources

Tissue-resident microbiota impacts colorectal cancer progression and prognosis [PDF]

Nature Communications
To deepen the understanding of tissue-resident microbiota in colorectal cancer (CRC), we analyzed whole-genome and transcriptome data from 937 patients. We identified 249 genera and 361 species commonly present in both tumors and adjacent normal tissues (
Zhun Shi, Huahui Ren, Cong Lin, Fuqiang Li, Meizhen Wu, Fangming Yang, Tian Luo, Luís Nunes, Anders Isaksson, Klara Hammarström, Ting Zhu, Shida Zhu, Yiyi Zhong, Ingrid Ljuslinder, Mathias Uhlén, Richard Palmqvist, Bengt Glimelius, Kui Wu, Tobias Sjöblom, Huanzi Zhong   +19 more
doaj   +2 more sources

PKS-ISLAND POSITIVE E. COLI AS MARKER OF NEGATIVE RESPONSE TO ANTI-PD1 THERAPY IN COLORECTAL CANCER

, 2019
A method of predicting resistance to anti-PD1 therapy in a subject suffering from cancer comprising the steps of a) determining, from a biological sample from a subject, in particular a feces or colonic biopsy sample from the subject, the presence of a bacterial pks island, b) predicting from the result of step a) that the subject is likely to be ...
Bonnet, Mathilde, Billard, Elisabeth, Dumas, Bruno, Lopès, Amélie   +3 more
openaire   +2 more sources

Prevalence and Molecular Characteristics of <i>Klebsiella pneumoniae</i> Harboring the <i>Pks</i> Island from Cancer Patients in China. [PDF]

Infect Drug Resist
Klebsiella pneumoniae harbors a gene cluster, polyketide synthase island (PKS), which is responsible for colibactin synthesis which induces double-stranded DNA breaks and associated with increased pathogenicity and cancer development. However, there is limited information on pks-positive K. pneumoniae in cancer patients in China.
Wang Q, Wang X, Xie Z, Qu J, Lin Y, Hu J, Li L, Zhang L.   +7 more
europepmc   +5 more sources

D-Serine reduces the expression of the cytopathic genotoxin colibactin

Microbial Cell, 2023
Some Escherichia coli strains harbour the pks island, a 54 kb genomic island en-coding the biosynthesis genes for a genotoxic compound named colibactin. In eukaryotic cells, colibactin can induce DNA damage, cell cycle arrest and chro-mosomal instability.
Jennifer C. Hallam, Sofia Sandalli, Iris Floria, Natasha C. A. Turner, Min Tang-Fichaux, Eric Oswald, Nicky O’Boyle, Andrew J. Roe   +7 more
doaj   +1 more source

Robust performance of a live bacterial therapeutic chassis lacking the colibactin gene cluster.

PLoS ONE, 2023
E. coli Nissle (EcN) is a non-pathogenic probiotic bacterium of the Enterobacteriaceae family that has been used for over a century to promote general gut health.
Aida Kalantari, Michael J James, Lauren A Renaud, Mylene Perreault, Catherine E Monahan, Mary N McDonald, David L Hava, Vincent M Isabella   +7 more
doaj   +1 more source