High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells [PDF]
Journal of Visualized Experiments, 2019 In their physiological environment, mammalian cells are often subjected to mechanical and biochemical stresses that result in plasma membrane damage. In response to these damages, complex molecular machineries rapidly reseal the plasma membrane to restore its barrier function and maintain cell survival.
Stephanie Seveau, Chi Song
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Frontiers in Cell and Developmental Biology, 2022 After damage, cells repair their plasma membrane in an active process that is driven by Ca2+ entering through the wound. This triggers a range of Ca2+-regulated events such as the translocation of different Ca2+-binding proteins to the wound site which ...
Arsila P. K. Ashraf, Volker Gerke
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Membrane Tension Regulation is Required for Wound Repair [PDF]
Advanced ScienceDisruptions of the eukaryotic plasma membrane due to chemical and mechanical challenges are frequent and detrimental and thus need to be repaired to maintain proper cell function and avoid cell death.
Nikita Raj +7 more
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Plasma Membrane Repair Is Regulated Extracellularly by Proteases Released from Lysosomes. [PDF]
PLoS ONE, 2016 Eukaryotic cells rapidly repair wounds on their plasma membrane. Resealing is Ca(2+)-dependent, and involves exocytosis of lysosomes followed by massive endocytosis.
Thiago Castro-Gomes +3 more
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Hypercapnic Acidosis Impairs Plasma Membrane Wound Resealing in Ventilator-injured Lungs [PDF]
American Journal of Respiratory and Critical Care Medicine, 2005 Abstract The objective of this study was to assess the effects of hypercapnic acidosis on lung cell injury and repair by confocal microscopy in a model of ventilator-induced lung injury. Three groups of normocapnic, hypocapnic, and hypercapnic rat lungs were perfused ex vivo, either during or after injurious ventilation, with a ...
, Rolf D Hubmayr
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Rapid Increase in Plasma Membrane Chloride Permeability during Wound Resealing in Starfish Oocytes [PDF]
Journal of General Physiology, 2005 Plasma membrane wound repair is an important but poorly understood process. We used femtosecond pulses from a Ti-Sapphire laser to make multiphoton excitation–induced disruptions of the plasma membrane while monitoring the membrane potential and resistance. We observed two types of wounds that depolarized the plasma membrane. At threshold light levels,
Terasaki Mark
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Hydrogen Peroxide-Mediated Inhibition of Membrane Resealing Drives Synergistic Cytotoxicity of Combined Cold Atmospheric Plasma and Pulsed Electric Field Treatment. [PDF]
Int J Mol SciCold atmospheric plasma (CAP) combined with pulsed electric fields (PEF) demonstrates synergistic cytotoxicity against HeLa cells; however, the differential contributions of short-lived versus long-lived reactive oxygen and nitrogen species (RONS) remain unclear.
Kushibiki S, Kurita H.
europepmc +3 more sources
Nanosecond pulsed electric fields induce cell-size-dependent selective permeabilization of urothelial cancer cells [PDF]
Communications BiologyPlasma membrane integrity is vital for cell viability, yet its controlled disruption enables targeted delivery of therapeutic agents. Here, we examined membrane durability and repair capacity in normal and malignant urothelial cells using short, high ...
Aleksander Kielbik +14 more
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Polymorphonuclear leukocytes (PMNs) use different, strain-dependent mechanisms to kill the parasite Trichomonas vaginalis [PDF]
mBioThe unicellular parasite Trichomonas vaginalis (Tv) causes trichomoniasis, the most common non-viral sexually transmitted infection in humans. Trichomoniasis symptoms and sequelae vary substantially, and in vitro analyses have shown that strains differ ...
Frances Mercer +4 more
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Preparation of resealed pig lymphocyte plasma-membrane vesicles [PDF]
Biochemical Journal, 1980 Pig lymphocyte plasma-membrane vesicles that are impermeable to large molecules were prepared by means of centrifugation on a continuous dextran gradient. They were isolated in a yield of 60–80% of the total plasma membrane recovered, and were characterized as being impermeable to macromolecules by their exclusion of inulin (mol.wt.
A P, Johnstone, M J, Crumpton
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