Frontiers in Cell and Developmental Biology, 2022 After damage, cells repair their plasma membrane in an active process that is driven by Ca2+ entering through the wound. This triggers a range of Ca2+-regulated events such as the translocation of different Ca2+-binding proteins to the wound site which ...
Arsila P. K. Ashraf, Volker Gerke
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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells. [PDF]
J Vis Exp, 2019 In their physiological environment, mammalian cells are often subjected to mechanical and biochemical stresses that result in plasma membrane damage. In response to these damages, complex molecular machineries rapidly reseal the plasma membrane to restore its barrier function and maintain cell survival.
Lam JGT, Song C, Seveau S.
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Membrane Tension Regulation is Required for Wound Repair [PDF]
Advanced ScienceDisruptions of the eukaryotic plasma membrane due to chemical and mechanical challenges are frequent and detrimental and thus need to be repaired to maintain proper cell function and avoid cell death.
Nikita Raj +7 more
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Excess muscle plasma membrane leak disrupts ECM content and shifts macrophage-mediated muscle repair [PDF]
JCI InsightPlasma membrane repair is critical for tissue integrity, especially for elongated contractile muscle cells. Genetically mediated defects in plasma membrane resealing produce persistent leak, leading to a disordered extracellular matrix (ECM). Loss of the
GaHyun Lee +18 more
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Nanosecond pulsed electric fields induce cell-size-dependent selective permeabilization of urothelial cancer cells [PDF]
Communications BiologyPlasma membrane integrity is vital for cell viability, yet its controlled disruption enables targeted delivery of therapeutic agents. Here, we examined membrane durability and repair capacity in normal and malignant urothelial cells using short, high ...
Aleksander Kielbik +14 more
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Polymorphonuclear leukocytes (PMNs) use different, strain-dependent mechanisms to kill the parasite Trichomonas vaginalis [PDF]
mBioThe unicellular parasite Trichomonas vaginalis (Tv) causes trichomoniasis, the most common non-viral sexually transmitted infection in humans. Trichomoniasis symptoms and sequelae vary substantially, and in vitro analyses have shown that strains differ ...
Frances Mercer +4 more
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Plasma Membrane Repair Is Regulated Extracellularly by Proteases Released from Lysosomes. [PDF]
PLoS ONE, 2016 Eukaryotic cells rapidly repair wounds on their plasma membrane. Resealing is Ca(2+)-dependent, and involves exocytosis of lysosomes followed by massive endocytosis.
Thiago Castro-Gomes +3 more
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Cell membrane disruption stimulates NO/PKG signaling and potentiates cell membrane repair in neighboring cells. [PDF]
PLoS ONE, 2012 Resealing of a disrupted plasma membrane at the micron-diameter range requires Ca(2+)-regulated exocytosis. Repeated membrane disruptions reseal more quickly than the initial wound, and this potentiation of membrane resealing persists for at least 24 ...
Tatsuru Togo
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Membranes, 2022 The plasma membrane separates the interior of the cells from the extracellular fluid and protects the cell from disruptive external factors. Therefore, the self-repairing capability of the membrane is crucial for cells to maintain homeostasis and survive
Xingyu Xia +5 more
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Short-term transcriptomic response to plasma membrane injury
Scientific Reports, 2021 Plasma membrane repair mechanisms are activated within seconds post-injury to promote rapid membrane resealing in eukaryotic cells and prevent cell death. However, less is known about the regeneration phase that follows and how cells respond to injury in
Swantje Christin Häger +7 more
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