Hydrogen Peroxide-Mediated Inhibition of Membrane Resealing Drives Synergistic Cytotoxicity of Combined Cold Atmospheric Plasma and Pulsed Electric Field Treatment. [PDF]
Int J Mol SciCold atmospheric plasma (CAP) combined with pulsed electric fields (PEF) demonstrates synergistic cytotoxicity against HeLa cells; however, the differential contributions of short-lived versus long-lived reactive oxygen and nitrogen species (RONS) remain unclear.
Kushibiki S, Kurita H.
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Preparation of resealed pig lymphocyte plasma-membrane vesicles [PDF]
Biochemical Journal, 1980 Pig lymphocyte plasma-membrane vesicles that are impermeable to large molecules were prepared by means of centrifugation on a continuous dextran gradient. They were isolated in a yield of 60–80% of the total plasma membrane recovered, and were characterized as being impermeable to macromolecules by their exclusion of inulin (mol.wt.
A P, Johnstone, M J, Crumpton
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Disruption-induced mucus secretion: repair and protection. [PDF]
PLoS Biology, 2006 When a cell suffers a plasma membrane disruption, extracellular Ca(2+) rapidly diffuses into its cytosol, triggering there local homotypic and exocytotic membrane fusion events.
K Miyake, T Tanaka, P L McNeil
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Deficient Sarcolemma Repair in ALS: A Novel Mechanism with Therapeutic Potential
Cells, 2022 The plasma membrane (sarcolemma) of skeletal muscle myofibers is susceptible to injury caused by physical and chemical stresses during normal daily movement and/or under disease conditions. These acute plasma membrane disruptions are normally compensated
Ang Li +6 more
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Extracellular Vesicle-Bound Bacterial Toxin Pneumolysin Triggers Membrane Engagement and Damage Beyond Canonical Pore Formation. [PDF]
J Extracell VesiclesBacterial toxin hijacks host microvesicles to breach cell membranes Microvesicles derived from challenged host cells carrying the pneumococcal pore‐forming toxin pneumolysin promote fusion with target cells and membrane permeabilisation. Our integrated experimental and computational study reveals a mechanism by which PLY‐lipid interactions enable ...
Sagilkumar AC +8 more
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Neuronal Plasma Membrane Integrity is Transiently Disturbed by Traumatic Loading
Neuroscience Insights, 2020 The acute response of neurons subjected to traumatic loading involves plasma membrane disruption, yet the mechanical tolerance for membrane compromise, time course, and mechanisms for resealing are not well understood.
Gustavo R Prado, Michelle C LaPlaca
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Large Plasma Membrane Disruptions Are Rapidly Resealed by Ca2+-dependent Vesicle–Vesicle Fusion Events [PDF]
The Journal of Cell Biology, 1997 A microneedle puncture of the fibroblast or sea urchin egg surface rapidly evokes a localized exocytotic reaction that may be required for the rapid resealing that follows this breach in plasma membrane integrity (Steinhardt, R.A,. G. Bi, and J.M. Alderton. 1994. Science (Wash. DC). 263:390–393).
M, Terasaki, K, Miyake, P L, McNeil
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Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage
Advanced Science, 2023 The plasma membrane of a cell is subject to stresses causing ruptures that must be repaired immediately to preserve membrane integrity and ensure cell survival.
Nikita Raj +9 more
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A resealed-cell system for analyzing pathogenic intracellular events: perturbation of endocytic pathways under diabetic conditions. [PDF]
PLoS ONE, 2012 Cell-based assay systems that can serve as cellular models of aberrant function in pathogenic organs would be novel and useful tools for screening drugs and clarifying the molecular mechanisms of various diseases.
Fumi Kano +4 more
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Biology Open, 2017 Disruption of cellular plasma membranes is a common event in many animal tissues, and the membranes are usually rapidly resealed. Moreover, repeated membrane disruptions within a single cell reseal faster than the initial wound in a protein kinase A (PKA)
Tatsuru Togo
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