Results 41 to 50 of about 14,970 (304)

Cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in Escherichia coli

Brazilian Journal of Medical and Biological Research, 1997
We cloned the streptokinase (STK) gene of Streptococcus equisimilis in an expression vector of Escherichia coli to overexpress the profibrinolytic protein under the control of a tac promoter. Almost all the recombinant STK was exported to the periplasmic
L. Avilán, A. Yarzábal, C. Jürgensen, M. Bastidas, J. Cruz, J. Puig   +5 more
doaj   +1 more source

Computer-aided engineering of staphylokinase toward enhanced affinity and selectivity for plasmin

Computational and Structural Biotechnology Journal, 2022
Cardio- and cerebrovascular diseases are leading causes of death and disability, resulting in one of the highest socio-economic burdens of any disease type.
Dmitri Nikitin, Jan Mican, Martin Toul, David Bednar, Michaela Peskova, Patricia Kittova, Sandra Thalerova, Jan Vitecek, Jiri Damborsky, Robert Mikulik, Sarel J. Fleishman, Zbynek Prokop, Martin Marek   +12 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Studies and perspectives of plasminogen activators

, 1992
The fibrinolytic system participates in a variety of physiological and pathological processes. It consists of an inactive proenzyme, plasminogen, which can be activated to the active serine protease plasmin by the action of different types of plasminogen
Ernst, B.; University of Rostock, Burstein, Christine; University of Rostock, Steiner, Michael; University of Rostock, Anders, Olaf; University of Rostock   +3 more
core   +1 more source

Reperfusion‐Dependent Outcomes After Endovascular Thrombectomy Stratified by NIHSS‐ASPECTS Clinical‐Core Mismatch

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective This analysis evaluates the effect of successful reperfusion on functional outcomes after MT, stratified by admission National Institutes of Health Stroke Scale (NIHSS) and Alberta Stroke Program Early CT Score (ASPECTS) as surrogates for clinical‐core mismatch, using multicenter registry data.
Felix Schlicht, Lukas Meyer, Gabriel Broocks, Matthias Bechstein, Christian Thaler, Christian Heitkamp, Laurens Winkelmeier, Vincent Geest, Alexander Heitkamp, Maximilian Jungnitz, Gregor Peter, Luca Meucci, Tobias Faizy, Jawed Nawabi, Caspar Brekenfeld, Fabian Flottmann, Maximilian Schell, Alexandros Hadjilaou, Uta Hanning, Götz Thomalla, Jens Fiehler, Susanne Gellißen, Helge C. Kniep, for the German Stroke Registry Endovascular Treatment, (GSR‐ET) Alexander Nave, Anna Alegiani, Annerose Mengel, Arno Reich, Bernd Eckert, Charlotte Pietrock, Christian Nolte, Eberhard Siebert, Fabian Flottmann, Fee Keil, Franziska Dorn, Gabor Petzold, Götz Thomalla, Hanna Zimmermann, Jan Borggrefe, Jan Hendrik Schäfer, Jens Fiehler, Joachim Röther, Klaus Gröschel, Lars Kellert, Mario Abruscato, Maximilian Schell, Omid Nikoubashman, Peter Schellinger, Silke Wunderlich, Steffen Tiedt, Sven Thonke, Timo Uphaus, Tobias Boeckh‐Behrens, Ulrike Ernemann   +53 more
wiley   +1 more source

THE RELEASE OF PLASMINOGEN ACTIVATORS FROM SPERMATOZOA DURING ACROSOME REACTION

, 1992
PLASMINOGEN ACTIVATORS ARE ENZYMES THAT CONVERT THE PROENZYME PLASMINOGEN TO THE TRYPSIN-LIKE PROTEINASE PLASMIN. PLASMINOGEN ACTIVATORS AND PLASMIN ARE REGULATED BY SEVERAL PLASMINOGEN ACTIVATOR INHIBITORS AND PLASMIN INHIBITORS, RESPECTIVELY. IN
Ταϊτζόγλου, Ιωάννης, Taitzoglou, Ioannis   +1 more
core   +1 more source

Comprehensive Assessment of Arterial, Tissue, and Venous Collaterals for Evaluating the Infarct Growth Rate: The Multimodal Collateral Score

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Collaterals are crucial factors that influence the infarct growth rate (IGR). We aimed to determine whether a comprehensive multimodal collateral score (MCS), incorporating collateral assessment at the arterial, tissue, and venous levels, is associated with functional independence and provides incremental prognostic value over ...
Giorgio Busto, Umberto Pensato, Ilaria Casetta, Giorgio U. Marcello, Angelo Barra, Anna Poggesi, Edoardo Carlesi, Sara Mancini, Andrea Ginestroni, Vittorio Miele, Alessandro Padovani, Andrea Morotti, Enrico Fainardi   +12 more
wiley   +1 more source

Precision targeting of the plasminogen activator inhibitor‐1 mechanism increases efficacy of fibrinolytic therapy in empyema

Physiological Reports, 2021
Plasminogen activator inhibitor‐1 (PAI‐1) is an endogenous irreversible inhibitor of tissue‐type (tPA) and urokinase (uPA) plasminogen activators. PAI‐1‐targeted fibrinolytic therapy (PAI‐1‐TFT) is designed to decrease the therapeutic dose of tPA and uPA,
Galina Florova, René A. Girard, Ali O. Azghani, Krishna Sarva, Ann Buchanan, Sophia Karandashova, Christian J. DeVera, Danna Morris, Mignote Chamiso, Kathleen Koenig, Douglas B. Cines, Steven Idell, Andrey A. Komissarov   +12 more
doaj   +1 more source

Microfabricated Anisotropic Myobundles for the Scalable Production of Cardiac Tissue Grafts

Advanced Functional Materials, EarlyView.
Controlling the anisotropy of cardiac tissue remains an outstanding challenge in the field of cardiac tissue engineering. Here, we introduce an approach to generate anisotropic cardiac myobundles using cell‐adhesive, synthetic, electrospun fibers and stem cell‐derived cardiac fibroblasts.
Maggie E. Jewett, Javiera Jilberto, Margaret E. Stanley, David O. Ntim, Siddhi M. Bhirud, Avianna T. Schroeder, Gonzola X. Anyosa Galvez, Jingyi Xia, Samuel J. DePalma, Emmanouil Agrafiotis, Susan S. Xi, Amanda S. Bluem, Courtney M. Lestock, Adam S. Helms, David A. Nordsletten, Brendon M. Baker   +15 more
wiley   +1 more source

Cross‐Scale Hierarchical Targeted Delivery System Based on Small‐Scale Magnetic Robots

Advanced Robotics Research, EarlyView.
This article reviews a cross‐scale hierarchical targeted delivery system that integrates magnetic continuum robots and magnetic microrobots. By combining rapid long‐range navigation with precise microscale targeting, the system overcomes key limitations of single‐scale approaches.
Junjian Zhou, Zhiqiang Chen, Yuanbiao Ma, Zhiqiang Tang, Qianqian Wang   +4 more
wiley   +1 more source