BMC Infectious Diseases, 2001 Background Pyrazinamide (PZA) is among the first-line drugs for the treatment of tuberculosis. In vitro, it kills semidormant mycobacteria only at low pH.
Kim Cheol Min +8 more
doaj +6 more sources
INSERSI GEN pncA KE DALAM PLASMID pGEM-T [PDF]
Jurnal Kimia Riset, 2016 . Multidrug-resistant tuberculosis (MDR-TB) is among the most worrisome elements of the pandemic of antibiotic resistance. As the first line drug, pyrazinamide is often used to treat TB desease so there are many case of TB resistant to pyrazinamide. The
Eli Hendrik Sanjaya
doaj +4 more sources
Characteristics of
BMC Infectious Diseases, 2011 Background Pyrazinamide (PZA) is an important first-line drug in multidrug-resistant tuberculosis (MDRTB) treatment. However, the unreliable results obtained from traditional susceptibility testing limits its usefulness in clinical settings.
Lee Yu-Chin +5 more
doaj +5 more sources
Novel Mutations in pncA Gene of Pyrazinamide Resistant Clinical Isolates of Mycobacterium tuberculosis [PDF]
Scientia Pharmaceutica, 2018 In clinical isolates of Mycobacterium tuberculosis (MTB), resistance to pyrazinamide occurs by mutations in any positions of the pncA gene (NC_000962.3) especially in nucleotides 359 and 374.
Manijeh Kahbazi +6 more
doaj +5 more sources
Structure guided prediction of Pyrazinamide resistance mutations in pncA [PDF]
Scientific Reports, 2020 AbstractPyrazinamide plays an important role in tuberculosis treatment; however, its use is complicated by side-effects and challenges with reliable drug susceptibility testing. Resistance to pyrazinamide is largely driven by mutations in pyrazinamidase (pncA), responsible for drug activation, but genetic heterogeneity has hindered development of a ...
Karmakar, Malancha +4 more
core +16 more sources
Analysis of pncA gene expression in pyrazinamide-resistant and multidrug-resistant Mycobacterium tuberculosis [PDF]
Xin yixue, 2023 Objective To compare the expression levels of pncA gene in pyrazinamide （PZA）-resistant and multidrug-resistant Mycobacterium tuberculosis before and after PZA drug stimulation， and to explore the relationship between PZA-resistant Mycobacterium ...
Wang Nan，, Yang Yu, Deng Ling, Wu Hui, Liu Zhihui, Meng Fanrong
doaj +3 more sources
Detection of Mutations in pncA in Mycobacterium tuberculosis Clinical Isolates from Nepal in Association with Pyrazinamide Resistance [PDF]
Current Issues in Molecular Biology, 2022 Without the proper information on pyrazinamide (PZA) susceptibility of Mycobacterium tuberculosis (MTB), PZA is inappropriately recommended for the treatment of both susceptible and multidrug-resistant tuberculosis (MDR-TB) in Nepal.
Dipti Shrestha +7 more
doaj +2 more sources
pncA gene expression and prediction factors on pyrazinamide resistance in Mycobacterium tuberculosis [PDF]
Tuberculosis, 2013 Mutations in the pyrazinamidase (PZAse) coding gene, pncA, have been considered as the main cause of pyrazinamide (PZA) resistance in Mycobacterium tuberculosis. However, recent studies suggest there is no single mechanism of resistance to PZA. The pyrazinoic acid (POA) efflux rate is the basis of the PZA susceptibility Wayne test, and its quantitative
Sheen, Patricia +7 more
openaire +6 more sources
pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Isolates in Portugal [PDF]
Antimicrobial Agents and Chemotherapy, 2004 ABSTRACT The nucleotide sequences of the pncA genes within 55 multidrug-resistant pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates were determined. Fifty-three out of the 55 isolates were pyrazinamidase (PZase) negative. Four strains contained a wild-type
Portugal, I +3 more
openaire +5 more sources
Estandarización de una técnica de complementación del gen pncA en Mycobacterium tuberculosis pncA-knockout: herramienta para el estudio de la relación entre mutaciones en pncA y parámetros fenotípicos [PDF]
, 2019 Mutaciones en el gen pncA son la mayor causa de resistencia a pirazinamida (PZA), pero existen mecanismos alternativos que también aportan resistencia. El objetivo del estudio fue estandarizar la técnica de complementación del gen pncA en Mycobacterium tuberculosis H37Rv pncA-KO para evaluar el efecto de las mutaciones en el gen pncA sobre parámetros ...
Cauna Orocollo, Yudith +2 more
core +5 more sources