Results 41 to 50 of about 2,913 (168)

A pncA polymorphism to differentiate between Mycobacterium bovis and Mycobacterium tuberculosis

Molecular and Cellular Probes, 2004
The pyrazinamidase gene coding for the enzyme that activates the bactericidal drug pyrazinamide contains a polymorphic site that is preserved in Mycobacterium bovis. We synthesized two sets of primers, one encompassing a 180 bp fragment, and the second spanning a 726 bp fragment including the full pncA gene.
A S, Barouni, C J, Augusto, M T P, Lopes, M S, Zanini, C E, Salas   +4 more
openaire   +2 more sources

Associations between Perceived Social Eating Norms and Initiation and Maintenance of Changes in Dietary Habits during the First COVID-19 Lockdown in France

Foods, 2021
Changes in dietary habits of the French population have been reported during the national lockdown that was enforced due to the COVID-19 pandemic. This study investigated whether perceived social eating norms were associated with the initiation and ...
Armelle Garcia, Suzanne Higgs, Anne Lluch, Nicolas Darcel, Olga Davidenko   +4 more
doaj   +1 more source

Mycobacterium smegmatis Has Two Pyrazinamidase Enzymes, PncA and PzaA [PDF]

Journal of Bacteriology, 2000
ABSTRACT The Mycobacterium smegmatis pncA gene, encoding nicotinamidase/pyrazinamidase, was identified. While it was similar to counterparts from other mycobacteria, the M. smegmatis PncA had little homology to the other M.
M, Guo, Z, Sun, Y, Zhang
openaire   +2 more sources

Genetic Diversity and Drug Susceptibility Profiles of Multidrug-Resistant Tuberculosis Strains in Southeast China

Infection and Drug Resistance, 2021
Shufang Lin,1 Shuzhen Wei,1 Yong Zhao,1 Zhisong Dai,1 Jian Lin,1 Yu Pang2 1Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, People’s Republic of China; 2Department of Bacteriology and ...
Lin S, Wei S, Zhao Y, Dai Z, Lin J, Pang Y   +5 more
doaj  

Brucella abortus nicotinamidase (PncA) contributes to its intracellular replication and infectivity in mice [PDF]

FEMS Microbiology Letters, 2004
Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in professional and non-professional phagocytes, and cause abortion in domestic animals and undulant fever in humans. The mechanism and factors of virulence are not fully understood.
Suk, Kim, Daisuke, Kurokawa, Kenta, Watanabe, Sou-Ichi, Makino, Toshikazu, Shirahata, Masahisa, Watarai   +5 more
openaire   +2 more sources

Functional Sex Reversal in Adult Gonochoristic Teleosts is Mediated by Ovarian Germline Stem Cell Plasticity and Somatic Cell Transdifferentiation

Advanced Science, EarlyView.
Oocytes play crucial roles in maintaining ovarian fate in adult gonochoristic fish. Targeted oocyte ablation with aromatase inhibition triggers the activation of surviving germline stem cells and the transdifferentiation of somatic cells from granoulosa/theca to Sertoli/Leydig lineages, inducing a complete and functional female‐to‐male sex reversal ...
Yang Yang, Liechao Yu, Wanchang Zhang, Gary Wessel, Ruiyi Chen, Weihua Hu, Dongdong Xu   +6 more
wiley   +1 more source

Mycobacterium tuberculosis Pyrazinamide Resistance Determinants: a Multicenter Study

mBio, 2014
Pyrazinamide (PZA) is a prodrug that is converted to pyrazinoic acid by the enzyme pyrazinamidase, encoded by the pncA gene in Mycobacterium tuberculosis.
Paolo Miotto, Andrea M. Cabibbe, Silke Feuerriegel, Nicola Casali, Francis Drobniewski, Yulia Rodionova, Daiva Bakonyte, Petras Stakenas, Edita Pimkina, Ewa Augustynowicz-Kopeć, Massimo Degano, Alessandro Ambrosi, Sven Hoffner, Mikael Mansjö, Jim Werngren, Sabine Rüsch-Gerdes, Stefan Niemann, Daniela M. Cirillo   +17 more
doaj   +1 more source

Methods of Assessing Postoperative Pain in Infants (0–12 Months): A Scoping Review

Paediatric and Neonatal Pain, Volume 8, Issue 3, September 2026.
ABSTRACT Infants undergoing surgery experience postoperative pain beyond acute short‐lasting procedural pain and have variable analgesic needs. Assessing pain in this nonverbal population presents challenges for clinicians and parents. Given the short‐ and long‐term effects of pain and its treatment, timely and accurate assessment of pain is vital. The
Jeewan Jyoti, Donna Waters, Sharon Laing, Denise Harrison, Nadia Badawi, Himanshu Popat   +5 more
wiley   +1 more source

Rapid Sequencing of the Mycobacterium tuberculosis pncA Gene for Detection of Pyrazinamide Susceptibility [PDF]

Journal of Clinical Microbiology, 2014
ABSTRACT We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous ...
Streicher, Elizabeth M., Maharaj, Kashmeel, York, Talita, Van Heerden, Carel, Barnard, Marinus, Diacon, Andreas, Mendel, Carl M., Bosman, Marlein E., Hepple, Juli A., Pym, Alexander S., Warren, Robin M., Van Helden, Paul D.   +11 more
openaire   +3 more sources

A Nanoparticle‐Integrated Complete Manufacturing Pipeline of Chemically Engineered Exosomes

Advanced Science, Volume 13, Issue 32, 9 June 2026.
We report a novel manufacture technology of chemically engineered exosomes. The four steps of manufacturing, i.e., biogenesis, loading, isolation, and storage, are integrated by the use of a nanoparticle. The manufacture technology incorporates three innovative components, i.e., a new nano‐bio effect, a new composite nanoparticle, and a new isolation ...
Xiaowei Wen, Zixing Xu, Zerun Hao, Yanming Chen, Kai Xie, Haofan Yin, Xueying Wang, Jie Min, Sihan Sun, Baiding Chen, Chengxiu Ling, Mingming Xu, Yizhao Chen, Gang Ruan   +13 more
wiley   +1 more source