Biomolecules, 2021 We developed two human-induced pluripotent stem cell (hiPSC)/human embryonic stem cell (hESC)-specific glycan-recognizing mouse antibodies, R-10G and R-17F, using the Tic (JCRB1331) hiPSC line as an antigen. R-10G recognizes a low-sulfate keratan sulfate,
Yuko Nagai +8 more
doaj +1 more source
Complex polarity: building multicellular tissues through apical membrane traffic [PDF]
, 2016 The formation of distinct subdomains of the cell surface is crucial for multicellular organism development. The most striking example of this is apical-basal polarization.
Adams +157 more
core +1 more source
PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry [PDF]
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 2017 Podocalyxin (PODXL) is a CD34-related sialomucin and a well-known marker of embryonic stem cells. PODXL is expressed in many types of tumors including colorectal cancers, breast cancers, and brain tumors. Overexpression of PODXL is an independent predictor of progression, metastasis, and poor outcome.
Ogasawara, Satoshi +9 more
openaire +2 more sources
Development of 'Redox Arrays' for identifying novel glutathionylated proteins in the secretome [PDF]
, 2015 Proteomics techniques for analysing the redox status of individual proteins in complex mixtures tend to identify the same proteins due to their high abundance.
Bottazzi, Barbara +6 more
core +1 more source
Counting glomeruli in human kidney specimens using ex vivo MRI without contrast agents. [PDF]
Magn Reson MedAbstract Purpose The total number of glomeruli is an important indicator of kidney function and susceptibility to disease. Glomeruli, however, are difficult to image using MRI without contrast enhancement. For this reason, previous studies have used cationic ferritin as a contrast agent to detect glomeruli in ex vivo and in vivo perfused kidneys.
Kurniawan ND +11 more
europepmc +2 more sources
Podocalyxin inhibits human embryo implantation in vitro and luminal podocalyxin in putative receptive endometrium is associated with implantation failure in fertility treatment [PDF]
Fertility and Sterility, 2021 To study whether endometrial epithelial podocalyxin (PCX) inhibits implantation of human embryos in vitro and in patients undergoing in vitro fertilization (IVF).We have recently identified PCX as a key negative regulator of endometrial epithelial receptivity.
Sophea Heng +9 more
openaire +3 more sources
Pharmaceutics, 2020 Cell-surface proteins that can endocytose into brain microvascular endothelial cells serve as promising candidates for receptor-mediated transcytosis across the blood–brain barrier (BBB).
Shingo Ito +6 more
doaj +1 more source
Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion [PDF]
, 2012 Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that
Hodgson, Lorna +3 more
core +2 more sources
Release of podocalyxin into the extracellular space
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2011 Peer ...
Fernández, Darío +5 more
openaire +4 more sources
Atypical focal segmental glomerulosclerosis associated with a new PODXL nonsense variant
Molecular Genetics & Genomic Medicine, 2021 Background Podocalyxin (PODXL) is a highly sialylated adhesion glycoprotein that plays an important role in podocyte's physiology. Recently, missense and nonsense dominant variants in the PODXL gene have been associated with focal segmental ...
David Marx +10 more
doaj +1 more source