Results 51 to 60 of about 15,343 (151)

CRL4 DCAF1 ubiquitin ligase regulates PLK4 protein levels to prevent premature centriole duplication

Life Science Alliance
This study identifies the E3 ubiquitin ligase, CRL4DCAF1, as a new regulator of polo-like kinase 4, PLK4, protein levels in the G2 phase of the cell cycle to prevent premature duplication of centrioles.
Josina Grossmann, Anne-Sophie Kratz, Alina Kordonsky, Gali Prag, Ingrid Hoffmann   +4 more
doaj   +1 more source

From Cytoskeletal Remodeling to Oocyte Quality: The Emerging Role of Mechanics

Advanced Science, EarlyView.
Mechanical properties of the oocyte are increasingly recognized as critical determinants of meiotic success and developmental potential. How actomyosin remodeling controls oocyte mechanics, how these properties are altered in pathological conditions, and how they could serve as markers in reproductive medicine are examined.
Anastasia Shihabi, Rose Bulteau, Lucie Barbier, Marie‐Emilie Terret   +3 more
wiley   +1 more source

Polo-like kinase 4 homodimerization is not required for catalytic activation, autodestruction, or centriole assembly [PDF]

, 2021
Abstract Polo-like kinase 4 (Plk4) is the master-regulator of centriole assembly and cell cycle-dependent regulation of its activity maintains proper centrosome number. During most of the cell cycle, Plk4 levels are nearly undetectable due to its ability to autophosphorylate and trigger its own ubiquitin-mediated ...
John M. Ryniawec, Daniel W. Buster, Lauren K. Slevin, Cody J. Boese, Anastasia Amoiroglou, Spencer M. Dean, Kevin C. Slep, Gregory C. Rogers   +7 more
openaire   +1 more source

Experimental and Natural Induction of de novo Centriole Formation

Frontiers in Cell and Developmental Biology, 2022
In cycling cells, new centrioles are assembled in the vicinity of pre-existing centrioles. Although this canonical centriole duplication is a tightly regulated process in animal cells, centrioles can also form in the absence of pre-existing centrioles ...
Kasuga Takumi, Daiju Kitagawa
doaj   +1 more source

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Two Polo-like kinase 4 binding domains in Asterless perform distinct roles in regulating kinase stability [PDF]

Journal of Cell Biology, 2015
Plk4 (Polo-like kinase 4) and its binding partner Asterless (Asl) are essential, conserved centriole assembly factors that induce centriole amplification when overexpressed. Previous studies found that Asl acts as a scaffolding protein; its N terminus binds Plk4’s tandem Polo box cassette (PB1-PB2) and targets Plk4 to centrioles to initiate centriole ...
Klebba, Joseph E., Galletta, Brian J., Nye, Jonathan, Plevock, Karen M., Buster, Daniel W., Hollingsworth, Natalie A., Slep, Kevin C., Rusan, Nasser M., Rogers, Gregory C.   +8 more
openaire   +2 more sources

Overcoming intranasal delivery barriers with ultrastable polyzwitterionic siRNA nanocages for enhanced glioblastoma therapy

BMEMat, EarlyView.
A ROS‐responsive, zwitterionic nanocage enables stable, intranasal siRNA delivery to glioblastoma, promoting deep tumor penetration via non‐degradative pathways and trigeminal nerve transport. This platform achieves durable gene silencing and tumor suppression, offering a non‐invasive, storage‐stable strategy for treating glioma and other neurological ...
Jingwen Xie, Jingxing Si, Xiaobei Deng, Jing Tan, Ruijie Zhu, Jizhou Qi, Gaoxia Ma, Bin Wang, Yi Wang, Yunzhou Guo, Yuping Chen, Shiqun Shao, Youqing Shen   +12 more
wiley   +1 more source

LY354740 Reduces Extracellular Glutamate Concentration, Inhibits Phosphorylation of Fyn/NMDARs, and Expression of PLK2/pS129 α-Synuclein in Mice Treated With Acute or Sub-Acute MPTP

Frontiers in Pharmacology, 2020
Glutamate overactivity in basal ganglia critically contributes to the exacerbation of dopaminergic neuron degeneration in Parkinson’s disease (PD). Activation of group II metabotropic glutamate receptors (mGlu2/3 receptors), which can decrease excitatory
Yang Tan, Yan Xu, Chi Cheng, Cong Zheng, Weiqi Zeng, Ji Wang, Xiaoqian Zhang, Xiaoman Yang, Jialing Wang, Xiaomei Yang, Shuke Nie, Xuebing Cao   +11 more
doaj   +1 more source

High‐Fat Diet Exacerbates Neuropathology in a Transgenic Mouse Model of Multiple System Atrophy

Movement Disorders, EarlyView.
Abstract Background Multiple system atrophy (MSA) is a rare and devastating neurodegenerative disorder. Accumulating clinical and preclinical evidence suggests that diabetes and insulin resistance may adversely influence MSA pathophysiology. Objective We investigated the potential association between diabetes, impaired glucose homeostasis, and MSA ...
Marie‐Laure Arotcarena, Anna Delamarre, Miguel Lopez‐Cuina, Hugo Martin, Elio Balpe, Xavier Fioramonti, Erwan Bezard, Wassilios G. Meissner   +7 more
wiley   +1 more source

Targeting Cell Cycle Vulnerabilities in Cancers: Emerging Strategies for Therapeutic Development

Cancer Science, EarlyView.
Dysregulated cell cycle control often involves alternative compensatory pathways in cancers to maintain its robustness but provide unique targetable vulnerabilities. We overview recent insights on cancer‐specific vulnerabilities across the cell cycle and discuss how these can be used to develop new therapeutic strategies.
Nana Kamakura, Minji Jo, Motoko Takahashi, Toru Hirota   +3 more
wiley   +1 more source