Results 211 to 220 of about 9,722 (255)
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, 2011 The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude extract are separated on a Heparine Sepharose™ column. The PARP-containing fractions are then affinity purified on a 3-
Amé, Jean-Christophe +3 more
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Plant Journal, 2001 Poly(ADP-ribos)ylation is a reversible protein modification that in higher plants is catalyzed by two structurally different poly(ADP-ribose) polymerases, App and Zap.
Elena Babiychuk +2 more
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Trapping Poly(ADP-Ribose) Polymerase
The Journal of Pharmacology and Experimental Therapeutics, 2015Recent findings indicate that a major mechanism by which poly(ADP-ribose) polymerase (PARP) inhibitors kill cancer cells is by trapping PARP1 and PARP2 to the sites of DNA damage. The PARP enzyme-inhibitor complex "locks" onto damaged DNA and prevents DNA repair, replication, and transcription, leading to cell death.
Yuqiao, Shen +2 more
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Poly(ADP-Ribose) Polymerase Inhibitors
Current Medicinal Chemistry, 2003Poly(ADP-ribose) polymerase-1 (PARP-1) is the principal member of the PARP enzyme family consisting of PARP-1 and several recently identified novel poly(ADP-ribosyl)ating enzymes. PARP-1 functions as a DNA damage sensor and signalling molecule. Upon binding to DNA breaks, activated PARP cleaves NAD(+) into nicotinamide and ADP-ribose and polymerizes ...
Garry J, Southan, Csaba, Szabó
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Poly(ADP-ribose) polymerases in double-strand break repair: Focus on PARP1, PARP2 and PARP3
Experimental Cell Research, 2014 Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins catalysed by Poly(ADP-ribose) polymerases (PARP). A wealth of recent advances in the biochemical and functional characterization of the DNA-dependent PARP family members
Carole Beck +2 more
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Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase
Biochemical and Biophysical Research Communications, 1997Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear.
M, Leist +5 more
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Structure and function of poly(ADP-ribose) polymerase
Molecular and Cellular Biochemistry, 1994Poly(ADP-ribose) polymerase (PARP) participates in the intricate network of systems developed by the eukaryotic cell to cope with the numerous environmental and endogenous genetoxic agents. Cloning of the PARP gene has allowed the development of genetic and molecular approaches to elucidate the structure and the function of this abundant and highly ...
Murcia, G +7 more
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Inhibition of poly(ADP-ribose) polymerase by arsenite
Mutation Research/Reviews in Mutation Research, 1997Inorganic arsenic is considered a human carcinogen based principally on epidemiological evidence. Unlike most initiating chemicals, arsenic is inactive or extremely weak in its ability to directly induce gene mutations. Arsenite has been shown, however, to enhance mutagenicity when present with other agents such as UV radiation.
J W, Yager, J K, Wiencke
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Cytological detection of poly (ADP-ribose) polymerase
Experimental Cell Research, 1976Abstract An attempt was made to demonstrate poly (ADP-ribose) polymerase cytologically. In vitro incorporation from the nucleotide, [ 3 H]NAD was detected in frozen sections of onion embryo and meristematic tissue by autoradiography. In meristematic tissue, there was a correlation between the number of cells displaying intensein vitro incorporation ...
J F, Payne, A K, Bal
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