Results 31 to 40 of about 882 (164)

Screening for Viruses in Indigenous Greek Black Pigs [PDF]

Microorganisms
The successful advancement of xenotransplantation has led to the development of highly sensitive detection systems for the screening of potentially zoonotic viruses in donor pigs and preventing their transmission to the recipient.
Hina Jhelum, Vasileios Papatsiros, Georgios Papakonstantinou, Ludwig Krabben, Benedikt Kaufer, Joachim Denner   +5 more
doaj   +2 more sources

Genomic Validation of PERV-C-Free Pigs to Support Xenotransplantation. [PDF]

Xenotransplantation
ABSTRACT Porcine endogenous retroviruses (PERVs) are present in the germ lines of domesticated pigs (Sus scrofa) and related suids. There are three types of PERVs, PERV‐A, ‐B, and ‐C, which differ in their host range. PERV‐A and ‐B can infect human and porcine cells, while PERV‐C only infects porcine cells. PERV‐A and ‐B are found in the genomes of all
Benjamin NR, Madrigal G, Ishida Y, Catchen J, Allen KL, Pepin B, Roca AL.   +6 more
europepmc   +2 more sources

Porcine Corneal Tissue and Xenozoonotic Risks: A Review of the Current Evidence. [PDF]

Xenotransplantation
ABSTRACT Corneal opacities affect millions worldwide, with corneal transplantation as the primary treatment. However, donor shortages remain a challenge, leaving thousands waiting for transplants. Xenotransplantation using porcine corneas has emerged as a promising alternative due to anatomical and physiological similarities with human corneas ...
Moreira R, Nascimento H, Martins TMDM, Barbieri G, Pires P, Carvalho LN, Rosa LR, Almeida A, Pessuti CL, Ferrer H, Gomes JÁP, Goulart E, Raia S, Belfort R.   +13 more
europepmc   +2 more sources

Removal of Porcine Endogenous Retroviruses in Decellularized Liver Bioscaffolds. [PDF]

Xenotransplantation
ABSTRACT Tissue engineering using decellularized liver scaffolds presents a promising approach in regenerative medicine, offering a potential alternative to donor organ transplantation. The use of human livers as a bioscaffold is restricted by their limited availability and quality.
van Hengel EVA, Drabek D, Roest HP, Willemse J, Reniers LJ, Stallmann H, de Jonge J, Grosveld FG, van der Laan LJW, Verstegen MMA.   +9 more
europepmc   +2 more sources

Liver Xenotransplantation: Ethical and Societal Implications. [PDF]

Xenotransplantation
ABSTRACT The shortage of transplantable livers and high waitlist mortality rates have accelerated the clinical translation of liver xenotransplantation. Recent milestones, including extracorporeal perfusion models and gene‐edited pig‐to‐human transplants in both decedents and living patients, indicate that clinical trials may be imminent.
Hurst DJ, Bobier CA, Padilla LA, Merlocco A, Meier RPH.   +4 more
europepmc   +2 more sources

Structure of the Core Postfusion Porcine Endogenous Retrovirus Fusion Protein

mBio, 2022
Retroviral elements from endogenous retroviruses have functions in mammalian physiology. The best-known examples are the envelope proteins that function in placenta development and immune suppression.
Trevor T. Dean, Vitor Hugo B. Serrão, Jeffrey E. Lee   +2 more
doaj   +1 more source

Detection of non-reference porcine endogenous retrovirus loci in the Vietnamese native pig genome

Scientific Reports, 2022
The Vietnamese native pig (VnP)—a porcine breed with a small body—has proven suitable as a biomedical animal model. Here, we demonstrate that, compared to other breeds, VnPs have fewer copies of porcine endogenous retroviruses (PERVs), which pose a risk ...
Shinya Ishihara, Masahiko Kumagai, Aisaku Arakawa, Masaaki Taniguchi, Ngo Thi Kim Cuc, Lan Doan Pham, Satoshi Mikawa, Kazuhiro Kikuchi   +7 more
doaj   +1 more source

Unexpected low expression of porcine endogenous retroviruses (PERVs) in porcine expanded potential stem cells (EPSCs)

Virus Research, 2021
Expanded potential stem cells (EPSCs) have been recently derived from porcine preimplantation embryos (Gao et al., 2019). These cells were shown to express key pluripotency genes, to be genetically stable and differentiate to derivatives of the three germ layers and additionally to trophoblast.
Luise, Krüger, Monika, Nowak-Imialek, Yannick, Kristiansen, Doris, Herrmann, Björn, Petersen, Joachim, Denner   +5 more
openaire   +2 more sources

The use of transgenic animals for xenotransplantation: An update. [PDF]

Animal Model Exp Med
One way to produce genetically modified pigs is to use somatic cell nuclear transfer (SCNT). This procedure involves removing the metaphase plate from a mature oocyte using micromanipulation and then transferring the modified nucleus from the donor somatic cell to the oocyte.
Motławska J, Amaral A, Cerveira-Pinto M, Kordowitzki P.   +3 more
europepmc   +2 more sources

Sensitive detection systems for infectious agents in xenotransplantation*

Xenotransplantation, EarlyView., 2020
Abstract Xenotransplantation of pig cells, tissues, or organs may be associated with transmission of porcine microorganisms, first of all of viruses, to the transplant recipient, potentially inducing a disease (zoonosis). I would like to define detection systems as the complex of sample generation, sample preparation, sample origin, time of sampling ...
Joachim Denner
wiley   +1 more source