Results 111 to 120 of about 7,617,750 (308)

Molecular genetic analysis of siRNA biogenesis and function in Arabidopsis thaliana [PDF]

, 2009
In diverse eukaryotes, small RNA products of Dicer-like (DCL) proteins regulate mRNA stability or translation, and direct chromatin modifications to genomic regions, phenomena collectively known as RNA silencing.
Blevins, Todd Lucas
core   +1 more source

Post-Transcriptional Gene Silencing in Transgenic Plants

, 1994
The recent successes of gene engineering in plants demonstrate the high potential of expressing transgenes in agronomically important crops. These approaches require that the introduced gene is stably expressed in subsequent plant generations. However, experiments involving transgenic plants show that the expression of chimeric genes can substantially ...
Carvalho Balieiro, Fabiano de, Boerjan, Wout, Ingelbrecht, I.L., Depicker, A., Inze, D., Montagu, M. van   +5 more
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Diverse functions of small RNAs in different plant-pathogen communications

Frontiers in Microbiology, 2016
RNA silencing is a conserved mechanism that utilizes small RNAs (sRNAs) to direct the regulation of gene expression at the transcriptional or post-transcriptional level.
Juan Huang, Meiling Yang, Xiaoming Zhang, Lu Lu   +3 more
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

PTGS is dispensable for the initiation of epigenetic silencing of an active transposon in Arabidopsis

EMBO Reports
Transposable elements (TEs) are repressed in plants through transcriptional gene silencing (TGS), maintained epigenetic silencing marks such as DNA methylation.
Marieke Trasser, Grégoire Bohl-Viallefond, Verónica Barragán-Borrero, Laura Diezma-Navas, Lukas Loncsek, Magnus Nordborg, Arturo Marí-Ordóñez   +6 more
doaj   +1 more source

Real-time transcriptional profiling of cellular and viral gene expression during lytic cytomegalovirus infection. [PDF]

, 2012
During viral infections cellular gene expression is subject to rapid alterations induced by both viral and antiviral mechanisms. In this study, we applied metabolic labeling of newly transcribed RNA with 4-thiouridine (4sU-tagging) to dissect the real ...
Lisa Marcinowski, Martina Rieder, Caroline C. Friedel (7812728), Marcinowski, Lisa, Lars Dölken, de Graaf, Miranda, Thomas Bonfert, Caroline C. Friedel (29226), Lars Dölken (138985), Györy, Ildiko, Caroline C Friedel, Philip Rosenstiel (68103), Jens B Bosse, Ruzsics, Zsolt, Ralf Zimmer, Bosse, Jens B., Lukas Windhager, Rosenstiel, Philip, Zsolt Ruzsics (75383), Jens B. Bosse (7812725), Zimmer, Ralf, Thomas Bonfert (138970), Rieder, Martina, Lisa Marcinowski (138962), Jens B. Bosse (138966), Friedel, Caroline C., Ildiko Györy (138972), Michael Lidschreiber, Ildiko Györy, Bernd Rädle (138968), Zsolt Ruzsics, Ralf Zimmer (21157), Windhager, Lukas, Miranda de Graaf, Philip Rosenstiel, Michael Lidschreiber (138963), Dölken, Lars, Olivia Prazeres da Costa, Prazeres da Costa, Olivia, Olivia Prazeres da Costa (138979), Bernd Rädle, Lukas Windhager (138964), Lidschreiber, Michael, Miranda de Graaf (138976), Bonfert, Thomas, Rädle, Bernd, Martina Rieder (138965)   +46 more
core   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Function of the upstream activating factor in chromatin structure organization and transcriptional regulation at the yeast ribosomal DNA [PDF]

, 2010
Chromatin is the template of all processes involved in DNA metabolism in the eukaryotic cell. Accordingly, chromatin is a dynamic structure which changes in its composition and posttranslational modification correlating with the functional state of a ...
Götze, Hannah
core   +1 more source

Clinical Impact of MGMT Promoter Methylation in IDH‐Mutant Gliomas: Influence of Threshold Selection and Clinical Confounding

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background The clinical relevance of MGMT promoter methylation in IDH‐mutant gliomas remains controversial in the era of molecular classification. We aimed to systematically evaluate its clinical relevance by integrating quantitative assessment, cutoff exploration, and adjustment for clinical confounding.
Haihui Jiang, Linxi Cao, Xiaodong Chen, Siqi Tong, Qinghui Zhu, Jun Yang, Song Lin, Yong Cui   +7 more
wiley   +1 more source