Results 41 to 50 of about 203,728 (290)

Understanding pre-mRNA splicing through crystallography [PDF]

Methods, 2017
Crystallography is a powerful tool to determine the atomic structures of proteins and RNAs. X-ray crystallography has been used to determine the structure of many splicing related proteins and RNAs, making major contributions to our understanding of the molecular mechanism and regulation of pre-mRNA splicing.
Sara, Espinosa, Lingdi, Zhang, Xueni, Li, Rui, Zhao   +3 more
openaire   +2 more sources

In Vitro Studies of the Prp9·Prp11·Prp21 Complex Indicate a Pathway for U2 Small Nuclear Ribonucleoprotein Activation [PDF]

, 1996
Pre-mRNA splicing takes place on a large ribonucleoprotein particle, the spliceosome which contains the five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6. In Saccharomyces cerevisiae the mRNA splicing factors, Prp9, Prp11, and Prp21,
Abelson, John, O'Day, Christine L., Wiest, Debra K.   +2 more
core   +1 more source

Rat Spinal Cord Injury Associated with Spasticity Leads to Widespread Changes in the Regulation of Retained Introns

Neurotrauma Reports, 2022
To determine molecular changes that correlate with long-term physiological changes after spinal cord injury associated with spasticity, we used a complete transection model with an injury at sacral spinal level S2, wherein tail spasms develop in rats ...
Samantha N. Hart, Samir P. Patel, Felicia M. Michael, Peter Stoilov, Chi Jing Leow, Alvaro G. Hernandez, Ariane Jolly, Pierre de la Grange, Alexander G. Rabchevsky, Stefan Stamm   +9 more
doaj   +1 more source

Modulation of PKM alternative splicing by PTBP1 promotes gemcitabine resistance in pancreatic cancer cells [PDF]

, 2015
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and incurable disease. Poor prognosis is due to multiple reasons, including acquisition of resistance to gemcitabine, the first-line chemotherapeutic approach.
Bielli, P, Calabretta, S, Capurso, G, DELLE FAVE, Gianfranco, Fendrich, V, Passacantilli, I, Pilozzi, Emanuela, Sette, C.   +7 more
core   +2 more sources

Unraveling the mechanism of recognition of the 3' splice site of the adenovirus major late promoter intron by the alternative splicing factor PUF60.

PLoS ONE, 2020
Pre-mRNA splicing is critical for achieving required amounts of a transcript at a given time and for regulating production of encoded protein. A given pre-mRNA may be spliced in many ways, or not at all, giving rise to multiple gene products.
Hsin-Hao T Hsiao, Gregg V Crichlow, James W Murphy, Ewa J Folta-Stogniew, Elias J Lolis, Demetrios T Braddock   +5 more
doaj   +1 more source

Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors [PDF]

, 1994
We have examined the functional significance of the organization of pre-mRNA splicing factors in a speckled distribution in the mammalian cell nucleus. Upon microinjection into living cells of oligonucleotides or antibodies that inhibit pre-mRNA splicing
Krainer, A. R., Mayeda, A., O'Keefe, R. T., Sadowski, C. L., Spector, D. L.   +4 more
core   +2 more sources

Defective splicing of the background K+ channel K2P5.1 by the pre-mRNA splicing inhibitor, pladienolide B in lectin-activated mouse splenic CD4+ T cells

Journal of Pharmacological Sciences, 2016
The two-pore domain K+ channel K2P5.1 has been implicated in the pathogenesis of autoimmune diseases. We investigated the changes in K2P5.1 activity caused by a defect in normal pre-mRNA splicing in concanavalin A-activated mouse splenic CD4+ T cells ...
Kazutaka Tagishi, Ayaka Shimizu, Kyoko Endo, Hiroaki Kito, Satomi Niwa, Masanori Fujii, Susumu Ohya   +6 more
doaj   +1 more source

Splicing of the U6 RNA precursor is impaired in fission yeast pre-mRNA splicing mutants [PDF]

, 1989
U6 RNA is a member of a class of small abundant stable nuclear RNAs that are essential for splicing. In all species examined so far, the U6 RNA is a RNA polymerase III transcript.
Potashkin, J., Frendewey, D.
core   +1 more source

Abnormal splicing of ABCA1 pre-mRNA in Tangier disease due to a IVS2 +5G>C mutation in ABCA1 gene

Journal of Lipid Research, 2003
Two point mutations of ABCA1 gene were found in a patient with Tangier disease (TD): i) G>C in intron 2 (IVS2 +5G>C) and ii) c.844 C>T in exon 9 (R282X).
Serena Altilia, Livia Pisciotta, Rita Garuti, Patrizia Tarugi, Alfredo Cantafora, Laura Calabresi, Jacopo Tagliabue, Sergio Maccari, Franco Bernini, Ilaria Zanotti, Carlo Vergani, Stefano Bertolini, Sebastiano Calandra   +12 more
doaj   +1 more source

Disassembly of interchromatin granule clusters alters the coordination of transcription and pre-mRNA splicing [PDF]

, 2002
To examine the involvement of interchromatin granule clusters (IGCs) in transcription and pre-mRNA splicing in mammalian cell nuclei, the serine-arginine (SR) protein kinase cdc2-like kinase (Clk)/STY was used as a tool to manipulate IGC integrity in ...
Sacco-Bubulya, P. A., Spector, D. L.
core   +1 more source