Results 21 to 30 of about 127,576 (188)

Accurate Processing of Human Pre-rRNA In Vitro [PDF]

Molecular and Cellular Biology, 1989
We report here that the mature 5' terminus of human 18S rRNA is generated in vitro by a two-step processing reaction. In the first step, SP6 transcripts were specifically cleaved in HeLa cell nucleolar extract at three positions near the external transcribed spacer (ETS)-18S boundary.
G J, Hannon, P A, Maroney, A, Branch, B J, Benenfield, H D, Robertson, T W, Nilsen   +5 more
openaire   +2 more sources

Effects of Hydrogen Peroxide Stress on the Nucleolar Redox Environment and Pre-rRNA Maturation

Frontiers in Molecular Biosciences, 2021
Identifying biologically relevant molecular targets of oxidative stress may provide new insights into disease mechanisms and accelerate development of novel biomarkers. Ribosome biogenesis is a fundamental prerequisite for cellular protein synthesis, but
Russell T. Sapio, Russell T. Sapio, Chelsea J. Burns, Dimitri G. Pestov   +3 more
doaj   +1 more source

Small Nucleolar RNAs and Pre-rRNA Processing in Plants [PDF]

The Plant Cell, 1998
Messenger RNA (mRNA) translation and thereby gene expression depend on the cell's ability to produce vast numbers of ribosomes, the major component of the translational machinery. The complexity inherent in the production of ribosomal RNAs (rRNAs), the key RNA components of the ribosome ...
J W, Brown, P J, Shaw
openaire   +2 more sources

The conserved RNA-binding protein Seb1 promotes cotranscriptional ribosomal RNA processing by controlling RNA polymerase I progression

Nature Communications, 2023
Transcription by RNA polymerase I (RNAPI) represents most of the transcriptional activity in eukaryotic cells and is associated with the production of mature ribosomal RNA (rRNA).
Maxime Duval, Carlo Yague-Sanz, Tomasz W. Turowski, Elisabeth Petfalski, David Tollervey, François Bachand   +5 more
doaj   +1 more source

Modification of Adenosine196 by Mettl3 Methyltransferase in the 5’-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation

Cells, 2020
Ribosome biogenesis is among the founding processes in the cell. During the first stages of ribosome biogenesis, polycistronic precursor of ribosomal RNA passes complex multistage maturation after transcription.
Olga Sergeeva, Philipp Sergeev, Pavel Melnikov, Tatiana Prikazchikova, Olga Dontsova, Timofei Zatsepin   +5 more
doaj   +1 more source

SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs [PDF]

, 1990
SSB-1, the yeast single-strand RNA-binding protein, is demonstrated to be a yeast nucleolar-specific, silver-binding protein. In double-label immunofluorescence microscopy experiments antibodies to two other nucleolar proteins, RNA Pol I 190-kD and ...
Abelson, John, Campbell, Judith, Clark, Michael W., Yip, Man Lun R.   +3 more
core   +2 more sources

A functional interface at the rDNA connects rRNA synthesis, pre-rRNA processing and nucleolar surveillance in budding yeast. [PDF]

PLoS ONE, 2011
Ribogenesis is a multistep error-prone process that is actively monitored by quality control mechanisms. How ribosomal RNA synthesis, pre-rRNA processing and nucleolar surveillance are integrated is unclear.
Nathalie Leporé, Denis L J Lafontaine
doaj   +1 more source

Lytic Reactivation of the Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) Is Accompanied by Major Nucleolar Alterations

Viruses, 2022
The nucleolus is a subnuclear compartment whose primary function is the biogenesis of ribosomal subunits. Certain viral infections affect the morphology and composition of the nucleolar compartment and influence ribosomal RNA (rRNA) transcription and ...
Nofar Atari, K. Shanmugha Rajan, Vaibhav Chikne, Smadar Cohen-Chalamish, Tirza Doniger, Odelia Orbaum, Avi Jacob, Inna Kalt, Shulamit Michaeli, Ronit Sarid   +9 more
doaj   +1 more source

Maturation of the 90S pre-ribosome requires Mrd1 dependent U3 snoRNA and 35S pre-rRNA structural rearrangements [PDF]

, 2018
In eukaryotes, ribosome biogenesis requires folding and assembly of the precursor rRNA (pre-rRNA) with a large number of proteins and snoRNPs into huge RNA-protein complexes. In spite of intense genetic, biochemical and high-resolution cryo-EM studies in
Barandun, Beltrame, Beltrame, Ben-shem, Borovjagin, Borovjagin, Borovjagin, Burlacu, Chaker-Margot, Cheng, Coleman, Dragon, Dutca, Elena Burlacu, Fredrik Lackmann, Gavin, Grandi, Granneman, Granneman, Gulen, Hartshorne, Hartshorne, Hector, Henras, Hoang, Hughes, Hughes, Intine, Jin, Kornprobst, Koš, Krogan, Kudla, Lars Wieslander, Longtine, Low, Lundkvist, Lusvarghi, Létoquart, Marmier-Gourrier, Mortimer, Méreau, Osheim, Pestov, Pérez-Fernández, Sahasranaman, Sander Granneman, Sardana, Sardana, Sardana, Segerstolpe, Segerstolpe, Selega, Sergey Belikov, Shah, Sharma, Sharma, Smola, Sun, Tang, Tschochner, Velichutina, Wang, Wieslander, Woolford, Yeh   +65 more
core   +1 more source

Synergistic interaction network between the snR30 RNP, Utp23, and ribosomal RNA during ribosome synthesis

RNA Biology, 2022
snR30/U17 is a highly conserved H/ACA RNA that is required for maturation of the small ribosomal subunit in eukaryotes. By base-pairing to the expansion segment 6 (ES6) of 18S ribosomal RNA (rRNA), the snR30 H/ACA Ribonucleoprotein (RNP) indirectly ...
Timothy J. Vos, Ute Kothe
doaj   +1 more source