Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms [PDF]
, 1992 A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigote-like forms in Schneider's Drosophila medium supplemented with 20% (v/v) foetal calf serum.
Coombs, G H +3 more
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promastigotes and axenic amastigotes
, 2008 Leishmania parasites survive despite exposure to the toxic nitrosative oxidants during phagocytosis by the host cell. In this work, the authors investigated comparatively the resistance of Leishmania amazonensis promastigotes and axenic amastigotes to a ...
Fabrino, Daniela Leite +15 more
core +1 more source
Clinical Case Reports, Volume 14, Issue 5, May 2026.ABSTRACT In patients with atypical ulcers and travel history to endemic areas, a comprehensive diagnostic workup for cutaneous leishmaniasis is imperative. This case confirms Leishmania tropica through combined microscopy, culture, and PCR, leading to successful treatment with systemic glucantime and adjunctive wound care.
Hossein Pazoki +3 more
wiley +1 more source
Resistance confirmation in in vivo recovered promastigotes.
, 2018 L. amazonensis promastigotes were recovered from the in vivo limiting dilution experiment from each treated group and cultivated with Schneider’s Drosophila medium.
Elmo E. Almeida-Amaral (619692) +2 more
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Distinct roles in autophagy and importance in infectivity of the two ATG4 cysteine peptidases of leishmania major [PDF]
, 2013 Macroautophagy in Leishmania, which is important for the cellular remodeling required during differentiation, relies upon the hydrolytic activity of two ATG4 cysteine peptidases (ATG4.1 and ATG4.2).
Coombs, G.H. +8 more
core +1 more source
Structural basis of secreted acid phosphatase polymerization in the Leishmania parasite
Protein Science, Volume 35, Issue 5, May 2026.Abstract Enzymes that assemble into filaments typically transition between protomeric and polymeric states in response to cellular conditions. In contrast, the secreted acid phosphatase (SAP) of Leishmania, one of the most abundant extracellular glycoproteins produced by the parasite and regarded as a major virulence factor in the neglected tropical ...
Priyanka Bose +4 more
wiley +1 more source
promastigotes by interferon–γ‐primed macrophages
, 2006 Leishmania donovani promastigotes evade the induction of a proinflammatory response during their invasion of naive macrophages. However,,their entry into IFN-gamma-primed macrophages is accompanied by the secretion of nitric oxide (NO) and ...
Frédéric Chano +5 more
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Live logarithmic phase Promastigotes of Leishmania major induced high level of INF-γ but lower level of IL-10 in whole blood culture of healthy individuals [PDF]
Acta Medica Iranica, 2000 Stage-specifci developmental forms of Lesihmania major promastigotes were grown in vitro. Procyclic and metacyclic promastigotes were cultured and separated by peanut agglutinin.
Kariminia A +3 more
doaj +2 more sources
In Vitro Evaluation of Antileishmanial Activity of Commiphora myrrha Essential Oil Nanoliposome
Veterinary Medicine and Science, Volume 12, Issue 3, May 2026.This study investigates the efficacy of nanoliposomal formulations containing myrrh essential oil in combating Leishmania protozoa in laboratory settings. Myrrh essential oil and nanoliposomes were prepared in RPMI‐1640 medium at concentrations of 1, 2, 5 and 10 µg/dL.
Zahra Akbari +2 more
wiley +1 more source
Investigating the role of the Leishmania (Leishmania) major HASP and SHERP genes during metacyclogenesis in the sand fly vectors, Phlebotomus (Phlebotomus) papatasi and Ph. (Ph.) duboscqi [PDF]
, 2013 Leishmania parasites are the causative agents of a diverse spectrum of infectious diseases termed the leishmaniases. These digenetic parasites exist as intracellular, aflagellate amastigotes in a mammalian host and as extracellular flagellated ...
Doehl, Johannes
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