Results 41 to 50 of about 1,531,051 (248)

ELISPOT assay to measure antigen-specific murine CD8(+) T cell responses.

, 2001
The enzyme-linked immunospot technique (ELISPOT) relies on the visualization of cytokine secretion by individual T cells following in vitro stimulation with antigen. This assay has been developed and standardized for the quantitative detection of antigen-
Luzia H Carvalho, Carvalho, LH, Fidel Zavala, Julius C.R Hafalla, Hafalla, JC, Zavala, F   +5 more
core   +1 more source

Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen. [PDF]

, 2001
We report that complete protection against malaria and total inhibition of liver stage development and parasitemia was obtained in 100% of BALB/c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein
Oscar Bruña-Romero, Gloria González-Aseguinolaza, Ruth S. Nussenzweig, Tsuji, M, González-Aseguinolaza, G, Julius C. R. Hafalla, Bruña-Romero, O, Hafalla, JC, Nussenzweig, RS, Moriya Tsuji   +9 more
core   +1 more source

Evaluation of Crude Anthrax Protective Antigen as an Adjutant For Brucella Abortus Vaccine In Mice [PDF]

Engineering and Technology Journal, 2012
Anthrax crude protective antigen (PA) was used in this study as an adjutant with Brucella abortus S19 strain vaccine in a ratio of 1:1 to immunize mice in dose of 0.1 ml s/c in order to decrease side effects of S19 vaccine and also to enhance its ...
Inam Jasim Lafta
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Passive Immunotherapy Protects against Enteric Invasion and Lethal Sepsis in a Murine Model of Gastrointestinal Anthrax

Toxins, 2015
The principal portal for anthrax infection in natural animal outbreaks is the digestive tract. Enteric exposure to anthrax, which is difficult to detect or prevent in a timely manner, could be exploited as an act of terror through contamination of human ...
Bruce Huang, Tao Xie, David Rotstein, Hui Fang, David M. Frucht   +4 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Characterization of proteins present in the biofilm matrix and outer membrane vesicles of Histophilus somni during iron-sufficient and iron-restricted growth: identification of potential protective antigens through in silico analyses

mBio
There is limited efficacy in vaccines currently available to prevent some animal diseases of bacterial origin, such as bovine respiratory disease caused by Histophilus somni.
Yue-Jia Lee, Mohd Abdullah, Yung-Fu Chang, Habeeb Al Sudani, Thomas J. Inzana   +4 more
doaj   +1 more source

Yersinia Outer Membrane Vesicles as Potential Vaccine Candidates in Protecting against Plague

Biomolecules, 2020
Despite the relatively low incidence of plague, its etiological agent, Yersinia pestis, is an exceptional epidemic danger due to the high infectivity and mortality of this infectious disease. Reports on the isolation of drug-resistant Y.
Andrey A. Byvalov, Ilya V. Konyshev, Vladimir N. Uversky, Svetlana V. Dentovskaya, Andrey P. Anisimov   +4 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source