Results 211 to 220 of about 42,092 (259)
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A Digest of Protein Purification
2010The isolation of a given protein, free of all other biomolecules, is the primary objective of any protein purification scheme. Classical chromatographic procedures have been designed to exploit particular distinguishing features of individual target proteins, such as size, physico-chemical properties and binding affinity.
Dermot, Walls +2 more
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On‐target ultrasonic digestion of proteins
PROTEOMICS, 2013In the present work, we report a novel on‐target protein cleavage method. The method utilizes ultrasonic energy and allows up to 20 samples to be cleaved in 5 min for protein identification and one sample in 30 s for on‐tissue digestion. The standard proteins were spotted on a conductive glass slide in a volume of 0.5 μL followed by 5 min of ...
Santos, Hugo M. +3 more
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Protein Digestibility and Ingestion
Nature, 1963BENDER1 has directed attention to the importance of the correct definition and usage of terms adopted in discussions on protein nutrition, in particular to the distinction between ‘apparent’ and ‘true’ as adjectives qualifying absorption and retention.
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Digestion and Absorption of Dietary Protein
Annual Review of Medicine, 1990Dietary protein is normally assimilated in an efficient manner following the action of gastrointestinal proteases. A number of pathological conditions can alter this process, with deleterious nutritional consequences.
R H, Erickson, Y S, Kim
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Enzymatic Digestion of Proteins in Solution
Current Protocols in Protein Science, 1995AbstractAnalysis of protein covalent structure is less complex and more accurate when performed on peptides derived from the larger protein. In contrast to acid‐promoted total hydrolysis, peptides are typically generated by selective proteolysis, i.e., by specifically cleaving peptide bonds with endoproteases that have varying degrees of specificity ...
L R, Riviere, P, Tempst
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Overalkylation of a Protein Digest with Iodoacetamide
Analytical Chemistry, 2001Cystine linkages in proteins are often opened with reducing agents, sometimes to improve their digestion, often to eliminate disulfide linkages from complicating analysis of the digest. After reduction, the sulfhydryls are usually reacted with iodoacetamide (IAM), iodoacetic acid (IAA), or another electrophile to prevent reformation of disulfide ...
E S, Boja, H M, Fales
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Influence of Kiwifruit on Protein Digestion
2013Green kiwifruit consumption has long been thought to assist in the digestion of food proteins due to the presence of the proteolytic enzyme actinidin. This chapter reviews the recent findings of both in vitro and in vivo studies on the effect of green kiwifruit (which contain the enzyme actinidin) on the gastro- and small-intestinal digestion of a ...
Lovedeep, Kaur, Mike, Boland
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Development and Adaptation of Protein Digestion
The Journal of Nutrition, 1985Protein digestion is a complex process in which most aspects have a developmental pattern of activity. Gastric pH and intestinal peptide and amino acid transport as well as the activities of pepsinogen, trypsin, chymotrypsin, enterokinase and intestinal dipeptidases vary during development.
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The Digestion of Proteins in Vivo
The Journal of Nutrition, 1955E S, NASSET, P, SCHWARTZ, H V, WEISS
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Protein Digestion for DIGE Analysis
2017In-gel digestion of protein spots derived from two-dimensional gels and their subsequent identification by mass spectrometry is involved in a multitude of mass spectrometry-driven proteomic experiments, including fluorescence difference gel electrophoresis (DIGE).
Sandra, Murphy, Kay, Ohlendieck
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