Frontiers in Microbiology, 2020 Flagella are bacterial organelles of locomotion. Their biogenesis is highly coordinated in time and space and relies on a specialized flagellar type III secretion system (fT3SS) required for the assembly of the extracellular hook, rod, and filament parts
John C. Hook +9 more
doaj +1 more source
Head-head interactions of resting myosin crossbridges in intact frog skeletal muscles, revealed by synchrotron x-ray fiber diffraction. [PDF]
PLoS ONE, 2012 The intensities of the myosin-based layer lines in the x-ray diffraction patterns from live resting frog skeletal muscles with full thick-thin filament overlap from which partial lattice sampling effects had been removed were analyzed to elucidate the ...
Kanji Oshima +3 more
doaj +1 more source
The molecular mechanism of load adaptation by branched actin networks
eLife, 2022 Branched actin networks are self-assembling molecular motors that move biological membranes and drive many important cellular processes, including phagocytosis, endocytosis, and pseudopod protrusion.
Tai-De Li +4 more
doaj +1 more source
Heterologous protein production in filamentous fungi
Applied Microbiology and Biotechnology, 2023 Abstract Filamentous fungi are able to produce a wide range of valuable proteins and enzymes for many industrial applications. Recent advances in fungal genomics and experimental technologies are rapidly changing the approaches for the development and use of filamentous fungi as hosts for the production of both ...
Dujuan Liu +2 more
openaire +6 more sources
Activation of ADF/cofilin by phosphorylation-regulated Slingshot phosphatase is required for the meiotic spindle assembly in Xenopus laevis oocytes [PDF]
, 2013 We identify Xenopus ADF/cofilin (XAC) and its activator, Slingshot phosphatase (XSSH), as key regulators of actin dynamics essential for spindle microtubule assembly during Xenopus oocyte maturation.
Abe, Hiroshi +9 more
core +1 more source
In Vitro Autonomous Construction of the Flagellar Axial Structure in Inverted Membrane Vesicles
Biomolecules, 2020 The bacterial flagellum is a filamentous organelle extending from the cell surface. The axial structure of the flagellum consists of the rod, hook, junction, filament, and cap.
Hiroyuki Terashima +5 more
doaj +1 more source
Cysteine-rich protein 2 accelerates actin filament cluster formation. [PDF]
PLoS ONE, 2017 Filamentous actin (F-actin) forms many types of structures and dynamically regulates cell morphology and movement, and plays a mechanosensory role for extracellular stimuli. In this study, we determined that the smooth muscle-related transcription factor,
Takanori Kihara +4 more
doaj +1 more source
Phosphorylation of the Arp2 subunit relieves auto-inhibitory interactions for Arp2/3 complex activation. [PDF]
, 2011 Actin filament assembly by the actin-related protein (Arp) 2/3 complex is necessary to build many cellular structures, including lamellipodia at the leading edge of motile cells and phagocytic cups, and to move endosomes and intracellular pathogens.
Barber, Diane L +3 more
core +3 more sources
Biochemical characterization of actin assembly mechanisms with ALS-associated profilin variants
European Journal of Cell Biology, 2022 Eight separate mutations in the actin-binding protein profilin-1 have been identified as a rare cause of amyotrophic lateral sclerosis (ALS). Profilin is essential for many neuronal cell processes through its regulation of lipids, nuclear signals, and ...
Xinbei Liu +4 more
doaj +1 more source
Homologous Recombination under the Single-Molecule Fluorescence Microscope [PDF]
, 2019 Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein ...
Dhakal, Soma, Gibbs, Dalton R.
core +2 more sources