Induced alternative splicing an opportunity to study PCSK9 protein isoforms at physiologically relevant concentrations [PDF]
Scientific Reports, 2023 Splice modulating antisense oligomers (AOs) are increasingly used to modulate RNA processing. While most are investigated for their use as therapeutics, AOs can also be used for basic research.
Jessica M. Cale +6 more
doaj +2 more sources
DeepLPI: a multimodal deep learning method for predicting the interactions between lncRNAs and protein isoforms [PDF]
BMC Bioinformatics, 2021 Background Long non-coding RNAs (lncRNAs) regulate diverse biological processes via interactions with proteins. Since the experimental methods to identify these interactions are expensive and time-consuming, many computational methods have been proposed.
Dipan Shaw +3 more
doaj +2 more sources
Brain clusterin protein isoforms and mitochondrial localization [PDF]
eLife, 2019 Clusterin (CLU), or apolipoprotein J (ApoJ), is the third most predominant genetic risk factor associated with late-onset Alzheimer’s disease (LOAD). In this study, we use multiple rodent and human brain tissue and neural cell models to demonstrate that ...
Sarah K Herring +4 more
doaj +2 more sources
Foot protein isoforms are expressed at different times during embryonic chick skeletal muscle development. [PDF]
, 1991 We have investigated the time course of expression of the alpha and beta triad junctional foot proteins in embryonic chick pectoral muscle. The level of [3H]ryanodine binding in muscle homogenates is low until day E20 of embryonic development, then ...
John L. Sutko +6 more
openalex +5 more sources
Alternative splicing in ABA signaling during seed germination
Frontiers in Plant Science, 2023 Seed germination is an essential step in a plant’s life cycle. It is controlled by complex physiological, biochemical, and molecular mechanisms and external factors.
Ewa Sybilska, Agata Daszkowska-Golec
doaj +1 more source
RNA Biology, 2022 Endothelial cells (ECs) comprise the lumenal lining of all blood vessels and are critical for the functioning of the cardiovascular system. Their phenotypes can be modulated by alternative splicing of RNA to produce distinct protein isoforms.
Madison M. Mehlferber +9 more
doaj +1 more source
On the Occurrence and Multimerization of Two-Polypeptide Phage Endolysins Encoded in Single Genes
Microbiology Spectrum, 2022 Bacteriophages (phages) and other viruses are extremely efficient in packing their genetic information, with several described cases of overlapping genes encoded in different open reading frames (ORFs).
Daniela Pinto +7 more
doaj +1 more source
Plant Proteoforms Under Environmental Stress: Functional Proteins Arising From a Single Gene
Frontiers in Plant Science, 2021 Proteins are directly involved in plant phenotypic response to ever changing environmental conditions. The ability to produce multiple mature functional proteins, i.e., proteoforms, from a single gene sequence represents an efficient tool ensuring the ...
Klára Kosová +4 more
doaj +1 more source
Cells, 2023 Synaptopodin-2 (SYNPO2) is a protein associated with the Z-disc in striated muscle cells. It interacts with α-actinin and filamin C, playing a role in Z-disc maintenance under stress by chaperone-assisted selective autophagy (CASA).
Keerthika Lohanadan +11 more
doaj +1 more source
Efficient Detection of the Alternative Spliced Human Proteome Using Translatome Sequencing
Frontiers in Molecular Biosciences, 2022 Alternative splicing (AS) isoforms create numerous proteoforms, expanding the complexity of the genome. Highly similar sequences, incomplete reference databases and the insufficient sequence coverage of mass spectrometry limit the identification of AS ...
Chun Wu +11 more
doaj +1 more source