Results 131 to 140 of about 32,987 (164)

Prenylation of proteins in Trypanosoma brucei

Molecular and Biochemical Parasitology, 1997
Prenyl modification of proteins by farnesyl and geranylgeranyl isoprenoids occurs in a variety of eukaryotic cells. Culturing of Trypanosoma brucei in the presence of [3H]mevalonolactone (which is hydrolyzed in cells to give mevalonic acid, the precursor of protein prenyl groups) and an inhibitor of mevalonic acid biosynthesis leads to the ...
K, Yokoyama, Y, Lin, K D, Stuart, M H, Gelb   +3 more
openaire   +2 more sources

Prenylation and G Protein Signaling

1994
Publisher Summary This chapter presents recent studies in the field of protein prenylation, with particular emphasis on the enzymology of these processes and the influence of the modifications on specific G proteins involved in cellular signaling. The known prenylated proteins include a number of GTP-binding regulatory proteins (G proteins); these ...
P J, Casey, J F, Moomaw, F L, Zhang, Y B, Higgins, J A, Thissen   +4 more
openaire   +2 more sources

Antigenic Prenylated Peptide Conjugates and Polyclonal Antibodies To Detect Protein Prenylation

Bioconjugate Chemistry, 2004
Posttranslational modification of proteins with farnesyl and geranylgeranyl groups is a required modification for signaling proteins that includes the small GTPases in the Ras, Rho, and Rab families, heterotrimeric G proteins, and nuclear lamin proteins.
Xiao-hui, Liu, Dae-Yeon, Suh, Jeffrey, Call, Glenn D, Prestwich   +3 more
openaire   +2 more sources

Analysis of the Stoichiometry of Rab Protein Prenylation

Analytical Biochemistry, 1995
Rab5 is a Ras-related GTP-binding protein that is post-translationally prenylated with the 20-carbon isoprenoid geranylgeranyl. We have developed a method to determine the stoichiometry of prenylation of Rab5, and Rab family members in general, based on the cell-free translation of these peptides in the presence or the absence of appropriate ...
J C, Sanford, L, Foster, Z, Kapadia, M, Wessling-Resnick   +3 more
openaire   +2 more sources

Inhibition of protein prenylation by metabolites of limonene

Biochemical Pharmacology, 1999
The monoterpenes limonene and perillyl alcohol are undergoing clinical evaluation in cancer patients. In this paper, we report the chemical synthesis, characterisation, and quantitation in patients' plasma of a novel human metabolite of limonene, which is identified as an isomer of perillic acid.
Hardcastle IR, Rowlands MG, Moreno BarberA, Grimshaw RM, Mohan MK, Nutley BP, Jarman M   +6 more
openaire   +3 more sources

Prenylated Proteins: the Structure of the Isoprenoid Group

Science, 1990
The mevalonate-derived portion of a prenylated protein from Chinese hamster ovary cells has been established as diterpenoid (C 20 ). This group is linked to a carboxyl-terminal cysteine as a thioether. It was removed from the protein by hydrazinolysis followed by Raney nickel desulfurization, and the resulting ...
H C, Rilling, E, Breunger, W W, Epstein, P F, Crain   +3 more
openaire   +2 more sources

Occurrence of Prenylated Proteins in Plant Cells

Biochemical and Biophysical Research Communications, 1993
In this paper evidence is presented for the occurrence of prenylated proteins in plants. When spinach leaves were incubated in the presence of [3H]mevalonate non-extractable lipids were found in the protein fraction after extraction with organic solvents.
E, Swiezewska, A, Thelin, G, Dallner, B, Andersson, L, Ernster   +4 more
openaire   +2 more sources

Protein Prenylation in Tetrahymena

Archiv für Protistenkunde, 1996
Summary Tetrahymena vorax V 2S and Tetrahymena pyriformis GL were examined for the presence of proteins covalently modified with mevalonate-derived lipids. Radiolabeling of cells using [3H]mevalonolactone, [ 3 H]mevalonate, [ 3 H]farnesyl pyrophosphate or [ 3 H]geranylgeranyl pyrophosphate resulted in the appearance of protein-associated ...
openaire   +1 more source

Prenylated Proteins in Regenerating Rat Liver

Biochemical and Biophysical Research Communications, 1994
During rat liver regeneration, at the onset of S-phase of the first cell cycle, an enhanced mevalonate incorporation into prenylated proteins is observed. The analysis of the lipid moieties of such proteins shows a 20-fold increased content of geraniol and farnesol. The expected increase of farnesylated and geranylgeranylated proteins in pre-S phase is
G, Bruscalupi, F, Castellano, S, Sacco, A, Trentalance   +3 more
openaire   +2 more sources

In Vitro Prenylation Assay of Arabidopsis Proteins

2013
Protein prenylation, like other lipid posttranslational modifications of eukaryotic proteins, plays important roles in protein-membrane association and protein-protein interactions. In Arabidopsis, hundreds of proteins involved in a great variety of biological processes are potential prenylation substrates that need to be verified, including ...
Wan, Shi, Qin, Zeng, Mark P, Running
openaire   +2 more sources