Results 1 to 10 of about 1,981,418 (307)
A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeast Saccharomyces cerevisiae [PDF]
Microbial Cell, 2023 Modular Cloning (MoClo) allows the combinatorial assembly of plasmids from standardized genetic parts without the need of error-prone PCR reactions. It is a very powerful strategy which enables highly flexible expression patterns without the need of ...
Pavel Simakin +2 more
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Protein-Targeting Drug Discovery [PDF]
Biomolecules, 2023 Protein-driven biological processes play a fundamental role in biomedicine because they are related to pathologies of enormous social impact, such as cancer, neuropathies, and viral diseases, including the one at the origin of the recent COVID-19 pandemic [...]
Vicidomini Caterina, Roviello Giovanni
europepmc +6 more sources
A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes [PDF]
eLifeThe majority of mitochondrial proteins are encoded in the nuclear genome. Many of them lack clear targeting signals. Therefore, what constitutes the entire mitochondrial proteome is still unclear. We here build on our previously developed bi-genomic (BiG)
Yury S Bykov +13 more
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Proteomics Identifies Substrates and a Novel Component in hSnd2-Dependent ER Protein Targeting [PDF]
Cells, 2022 Importing proteins into the endoplasmic reticulum (ER) is essential for about 30% of the human proteome. It involves the targeting of precursor proteins to the ER and their insertion into or translocation across the ER membrane. Furthermore, it relies on
Andrea Tirincsi +11 more
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Deubiquitinase-targeting chimeras for targeted protein stabilization [PDF]
Nature Chemical Biology, 2022 Many diseases are driven by proteins that are aberrantly ubiquitinated and degraded. These diseases would be therapeutically benefited by targeted protein stabilization (TPS). Here we present deubiquitinase-targeting chimeras (DUBTACs), heterobifunctional small molecules consisting of a deubiquitinase recruiter linked to a protein-targeting ligand, to ...
Nathaniel J. Henning +23 more
openaire +4 more sources
Molecules, 2023 The growing applications of peptide-based therapeutics require the development of efficient protocols from the perspective of an industrial scale-up. T3P® (cyclic propylphosphonic anhydride) promotes amidation in the solution-phase through a biomimetic ...
Alexia Mattellone +7 more
doaj +1 more source
Signal Transduction and Targeted Therapy, 2023 Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Identification of the underlying mechanism of HCC progression and exploration of new therapeutic drugs are urgently needed. Here, a compound library consisting of 419 FDA-approved
Xiang-Peng Tan +11 more
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Frontiers in Physiology, 2022 In human cells, approximately 30% of all polypeptides enter the secretory pathway at the level of the endoplasmic reticulum (ER). This process involves cleavable amino-terminal signal peptides (SPs) or more or less amino-terminal transmembrane helices ...
Sven Lang +5 more
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Advanced Science, 2022 Colorectal cancer (CRC) is one of the most common malignant tumors in the world, with high prevalence and low 5‐year survival. Most of the CRC patients show excessive activation of the Wnt/β‐catenin pathway which is a vital target for CRC treatment ...
Can‐Can Zheng +9 more
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How Quality Control Systems AID Sec-Dependent Protein Translocation
Frontiers in Molecular Biosciences, 2021 The evolutionarily conserved Sec machinery is responsible for transporting proteins across the cytoplasmic membrane. Protein substrates of the Sec machinery must be in an unfolded conformation in order to be translocated across (or inserted into) the ...
Chen Jiang, Max Wynne, Damon Huber
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