Results 21 to 30 of about 1,447,276 (313)

The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites

Nature Communications, 2017
The choice between homologous recombination and non-homologous end-joining is largely influenced by cell cycle. Here the authors show that APCCdh1 promotes homologous recombination by removing USP1, allowing polyubiquitinated histones to recruit BRCA1.
Kyungsoo Ha, Chengxian Ma, Han Lin, Lichun Tang, Zhusheng Lian, Fang Zhao, Ju-Mei Li, Bei Zhen, Huadong Pei, Suxia Han, Marcos Malumbres, Jianping Jin, Huan Chen, Yongxiang Zhao, Qing Zhu, Pumin Zhang   +15 more
doaj   +1 more source

Proteomics Identifies Substrates and a Novel Component in hSnd2-Dependent ER Protein Targeting

Cells, 2022
Importing proteins into the endoplasmic reticulum (ER) is essential for about 30% of the human proteome. It involves the targeting of precursor proteins to the ER and their insertion into or translocation across the ER membrane. Furthermore, it relies on
Andrea Tirincsi, Sarah O’Keefe, Duy Nguyen, Mark Sicking, Johanna Dudek, Friedrich Förster, Martin Jung, Drazena Hadzibeganovic, Volkhard Helms, Stephen High, Richard Zimmermann, Sven Lang   +11 more
doaj   +1 more source

An artificial immune system for clustering amino acids in the context of protein function classification [PDF]

, 2009
This paper addresses the classification task of data mining (a form of supervised learning) in the context of an important bioinformatics problem, namely the prediction of protein functions.
Timmis, Jon, Clark, Edward, Flower, Darren R., Secker, Andrew D., Davies, Matthew N., Freitas, Alex A.   +5 more
core   +1 more source

High confidence proteomic analysis of yeast LDs identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation[S]

Journal of Lipid Research, 2014
Accurate protein inventories are essential for understanding an organelle's functions. The lipid droplet (LD) is a ubiquitous intracellular organelle with major functions in lipid storage and metabolism.
Erin Currie, Xiuling Guo, Romain Christiano, Chandramohan Chitraju, Nora Kory, Kenneth Harrison, Joel Haas, Tobias C. Walther, Robert V. Farese, Jr.   +8 more
doaj   +1 more source

Co-Translational Protein Folding and Sorting in Chloroplasts

Plants, 2020
Cells depend on the continuous renewal of their proteome composition during the cell cycle and in order to replace aberrant proteins or to react to changing environmental conditions.
Fabian Ries, Claudia Herkt, Felix Willmund   +2 more
doaj   +1 more source

Quantitative Proteomics and Differential Protein Abundance Analysis after Depletion of Putative mRNA Receptors in the ER Membrane of Human Cells Identifies Novel Aspects of mRNA Targeting to the ER

Molecules, 2021
In human cells, one-third of all polypeptides enter the secretory pathway at the endoplasmic reticulum (ER). The specificity and efficiency of this process are guaranteed by targeting of mRNAs and/or polypeptides to the ER membrane. Cytosolic SRP and its
Pratiti Bhadra, Stefan Schorr, Monika Lerner, Duy Nguyen, Johanna Dudek, Friedrich Förster, Volkhard Helms, Sven Lang, Richard Zimmermann   +8 more
doaj   +1 more source

Identification of New Degrons in Streptococcus mutans Reveals a Novel Strategy for Engineering Targeted, Controllable Proteolysis

Frontiers in Microbiology, 2017
Recently, controllable, targeted proteolysis has emerged as one of the most promising new strategies to study essential genes and otherwise toxic mutations.
Nan Liu, Muhammad T. Chaudhry, Zhoujie Xie, Jens Kreth, Justin Merritt, Justin Merritt   +5 more
doaj   +1 more source

Early steps in mitochondrial protein import [PDF]

, 1990
The process of insertion of precursor proteins into mitochondrial membranes was investigated using a hybrid protein (pSc1-c) that contains dual targeting information and, at the same time, membrane insertion activity.
Stuart, Rosemary A., Neupert, Walter, Nicholson, Donald W.   +2 more
core   +1 more source

Unconventional Targeting of a Thiol Peroxidase to the Mitochondrial Intermembrane Space Facilitates Oxidative Protein Folding

Cell Reports, 2017
Thiol peroxidases are conserved hydrogen peroxide scavenging and signaling molecules that contain redox-active cysteine residues. We show here that Gpx3, the major H2O2 sensor in yeast, is present in the mitochondrial intermembrane space (IMS), where it ...
Paraskevi Kritsiligkou, Afroditi Chatzi, Georgia Charalampous, Aleksandr Mironov, Jr., Chris M. Grant, Kostas Tokatlidis   +5 more
doaj   +1 more source

Targeted protein degradation by Trim-Away using cell resealing coupled with microscopic image-based quantitative analysis

Frontiers in Cell and Developmental Biology, 2022
“Trim-Away” technology enables rapid degradation of endogenous proteins without prior modification of protein-coding genes or mRNAs through delivery of antibodies that target proteins of interest.
Rina Kunishige, Rina Kunishige, Masayuki Murata, Masayuki Murata, Masayuki Murata, Fumi Kano, Fumi Kano   +6 more
doaj   +1 more source