Results 1 to 10 of about 1,399,144 (265)

A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes. [PDF]

Elife
The majority of mitochondrial proteins are encoded in the nuclear genome. Many of them lack clear targeting signals. Therefore, what constitutes the entire mitochondrial proteome is still unclear. We here build on our previously developed bi-genomic (BiG)
Bykov YS, Zuttion S, Edilbi D, Polozova M, Arnold J, Malitsky S, Itkin M, Senger B, Klein O, Asraf Y, Meyer H, Becker HD, Kucharczyk R, Schuldiner M.   +13 more
europepmc   +2 more sources

Fast Solution-Phase and Liquid-Phase Peptide Syntheses (SolPSS and LPPS) Mediated by Biomimetic Cyclic Propylphosphonic Anhydride (T3P^®)

Molecules, 2023
The growing applications of peptide-based therapeutics require the development of efficient protocols from the perspective of an industrial scale-up. T3P® (cyclic propylphosphonic anhydride) promotes amidation in the solution-phase through a biomimetic ...
Alexia Mattellone, Dario Corbisiero, Paolo Cantelmi, Giulia Martelli, Chiara Palladino, Alessandra Tolomelli, Walter Cabri, Lucia Ferrazzano   +7 more
doaj   +1 more source

Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression

Signal Transduction and Targeted Therapy, 2023
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Identification of the underlying mechanism of HCC progression and exploration of new therapeutic drugs are urgently needed. Here, a compound library consisting of 419 FDA-approved
Xiang-Peng Tan, Yan He, Jing Yang, Xian Wei, You-Long Fan, Guo-Geng Zhang, Yi-Dong Zhu, Zheng-Qiu Li, Hua-Xin Liao, Da-Jiang Qin, Xin-Yuan Guan, Bin Li   +11 more
doaj   +1 more source

Signal Peptide Features Determining the Substrate Specificities of Targeting and Translocation Components in Human ER Protein Import

Frontiers in Physiology, 2022
In human cells, approximately 30% of all polypeptides enter the secretory pathway at the level of the endoplasmic reticulum (ER). This process involves cleavable amino-terminal signal peptides (SPs) or more or less amino-terminal transmembrane helices ...
Sven Lang, Duy Nguyen, Pratiti Bhadra, Martin Jung, Volkhard Helms, Richard Zimmermann   +5 more
doaj   +1 more source

Blockade of Nuclear β‐Catenin Signaling via Direct Targeting of RanBP3 with NU2058 Induces Cell Senescence to Suppress Colorectal Tumorigenesis

Advanced Science, 2022
Colorectal cancer (CRC) is one of the most common malignant tumors in the world, with high prevalence and low 5‐year survival. Most of the CRC patients show excessive activation of the Wnt/β‐catenin pathway which is a vital target for CRC treatment ...
Can‐Can Zheng, Long Liao, Ya‐Ping Liu, Yan‐Ming Yang, Yan He, Guo‐Geng Zhang, Shu‐Jun Li, Ting Liu, Wen Wen Xu, Bin Li   +9 more
doaj   +1 more source

How Quality Control Systems AID Sec-Dependent Protein Translocation

Frontiers in Molecular Biosciences, 2021
The evolutionarily conserved Sec machinery is responsible for transporting proteins across the cytoplasmic membrane. Protein substrates of the Sec machinery must be in an unfolded conformation in order to be translocated across (or inserted into) the ...
Chen Jiang, Max Wynne, Damon Huber
doaj   +1 more source

A Photosensitized Singlet Oxygen (¹O₂) Toolbox for Bio-Organic Applications: Tailoring ¹O₂ Generation for DNA and Protein Labelling, Targeting and Biosensing

Molecules, 2022
Singlet oxygen (1O2) is the excited state of ground, triplet state, molecular oxygen (O2). Photosensitized 1O2 has been extensively studied as one of the reactive oxygen species (ROS), responsible for damage of cellular components (protein, DNA, lipids).
Dorien Aerssens, Enrico Cadoni, Laure Tack, Annemieke Madder   +3 more
doaj   +1 more source

A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeast Saccharomyces cerevisiae

Microbial Cell, 2023
Modular Cloning (MoClo) allows the combinatorial assembly of plasmids from standardized genetic parts without the need of error-prone PCR reactions. It is a very powerful strategy which enables highly flexible expression patterns without the need of ...
Pavel Simakin, Christian Koch, Johannes M. Herrmann   +2 more
doaj   +1 more source

An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants

Plants, 2023
Protein targeting is essential in eukaryotic cells to maintain cell function and organelle identity. Signal peptides are a major type of targeting sequences containing a tripartite structure, which is conserved across all domains in life.
Tatiana Spatola Rossi, Verena Kriechbaumer   +1 more
doaj   +1 more source

Pls1 Is a Peroxisomal Matrix Protein with a Role in Regulating Lysine Biosynthesis

Cells, 2022
Peroxisomes host essential metabolic enzymes and are crucial for human health and survival. Although peroxisomes were first described over 60 years ago, their entire proteome has not yet been identified.
Yotam David, Inês Gomes Castro, Eden Yifrach, Chen Bibi, Enas Katawi, Dekel Yahav Har-Shai, Sagie Brodsky, Naama Barkai, Tommer Ravid, Miriam Eisenstein, Shmuel Pietrokovski, Maya Schuldiner, Einat Zalckvar   +12 more
doaj   +1 more source