Hereditary folate malabsorption due to a mutation in the external gate of the proton-coupled folate transporter SLC46A1 [PDF]
Blood Advances, 2018 : Hereditary folate malabsorption (HFM) is an autosomal recessive disorder characterized by impaired intestinal folate absorption and impaired folate transport across the choroid plexus due to loss of function of the proton-coupled folate transporter ...
Srinivas Aluri +5 more
doaj +6 more sources
Delineating the extracellular water-accessible surface of the proton-coupled folate transporter. [PDF]
PLoS ONE, 2013 The proton-coupled folate transporter (PCFT) was recently identified as the major uptake route for dietary folates in humans. The three-dimensional structure of PCFT and its detailed interplay with function remain to be determined.
Phaneendra Kumar Duddempudi +3 more
doaj +6 more sources
Biology and Therapeutic Applications of the Proton-Coupled Folate Transporter [PDF]
Expert Opinion on Drug Metabolism and Toxicology, 2022 The proton-coupled folate transporter (PCFT; SLC46A1) was discovered in 2006 as the principal mechanism by which folates are absorbed in the intestine and the causal basis for hereditary folate malabsorption (HFM). In 2011, it was found that PCFT is highly expressed in many tumors.
Zhanjun Hou +2 more
exaly +5 more sources
Targeted Delivery of Nanoparticulate Cytochrome C into Glioma Cells Through the Proton-Coupled Folate Transporter [PDF]
Biomolecules, 2019 In this study, we identified the proton-coupled folate transporter (PCFT) as a route for targeted delivery of drugs to some gliomas. Using the techniques of confocal imaging, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and ...
Yuriy V. Kucheryavykh +10 more
doaj +3 more sources
Expression in Sf9 insect cells, purification and functional reconstitution of the human proton-coupled folate transporter (PCFT, SLC46A1). [PDF]
PLoS ONE, 2017 The proton-coupled folate transporter (PCFT) provides an essential uptake route for the vitamin folic acid (B9) in mammals. In addition, it is currently of high interest for targeting chemotherapeutic agents to tumors due to the increased folic acid ...
Swapneeta S Date +3 more
doaj +3 more sources
Animal, 2013 Folate transporters, including the reduced folate carrier and the proton-coupled folate transporter, encoded by Slc19a1 and Slc46a1 genes respectively, play important roles in the transport of folate across biological membranes given the hydrophilic ...
M. Jing, G.B. Tactacan, J.D. House
doaj +5 more sources
Impact of nanodisc lipid composition on cell-free expression of proton-coupled folate transporter. [PDF]
PLoS ONE, 2021 The Proton-Coupled Folate Transporter (PCFT) is a transmembrane transport protein that controls the absorption of dietary folates in the small intestine. PCFT also mediates uptake of chemotherapeutically used antifolates into tumor cells.
Hoa Quynh Do +3 more
doaj +3 more sources
Experimentally Optimized Threading Structures of the Proton-Coupled Folate Transporter [PDF]
FEBS Open Bio, 2016 The proton‐coupled folate transporter (PCFT, SLC46A1) transports folic acid across the plasma membrane, together with an excess of protons such that the net charge translocation is positive. We developed 3D structural models of PCFT threaded onto the X‐ray structures of major facilitator superfamily (MFS) members that were identified as close ...
Swapneeta S Date, Michaela Jansen
exaly +5 more sources
The Proton-Coupled Folate Transporter (PCFT-SLC46A1) and the Syndrome of Systemic and Cerebral Folate Deficiency of Infancy: Hereditary Folate Malabsorption [PDF]
Molecular Aspects of Medicine, 2017 The proton-coupled folate transporter (PCFT-SLC46A1) is the mechanism by which folates are absorbed across the brush-border membrane of the small intestine. The transporter is also expressed in the choroid plexus and is required for transport of folates into the cerebrospinal fluid.
Srinivas Aluri +2 more
exaly +5 more sources
Role of Proton-Coupled Folate Transporter in Pemetrexed Resistance of Mesothelioma: Clinical Evidence and New Pharmacological Tools [PDF]
Annals of Oncology, 2017 Thymidylate synthase (TS) has a predictive role in pemetrexed treatment of mesothelioma; however, additional chemoresistance mechanisms are poorly understood. Here, we explored the role of the reduced-folate carrier (RFC/SLC19A1) and proton-coupled folate transporter (PCFT/SLC46A1) in antifolate resistance in mesothelioma.PCFT, RFC and TS RNA and PCFT ...
Michal Stark +2 more
exaly +7 more sources