Haem and folate transport by proton-coupled folate transporter/haem carrier protein 1 (SLC46A1) [PDF]
British Journal of Nutrition, 2008 Haem carrier protein 1 (HCP1) was originally identified and characterised as a mammalian haem transporter. However, recent evidence has shown that it is also a proton-coupled folate transporter (PCFT) and mutations in the gene cause hereditary folate deficiency in humans.
Laftah, Abas H +5 more
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Inhibition of the proton-coupled folate transporter (PCFT-SLC46A1) by bicarbonate and other anions. [PDF]
Mol Pharmacol, 2013 The proton-coupled folate transporter (PCFT) plays a key role in intestinal folate absorption, and loss-of-function mutations in the gene encoding this transporter are the molecular basis for hereditary folate malabsorption. Using a stable transfectant with high expression of PCFT, physiologic levels of bicarbonate produced potent and rapidly ...
Zhao R +3 more
europepmc +5 more sources
Folate transporter dynamics and therapy with classic and tumor-targeted antifolates
Scientific Reports, 2021 There are three major folate uptake systems in human tissues and tumors, including the reduced folate carrier (RFC), folate receptors (FRs) and proton-coupled folate transporter (PCFT). We studied the functional interrelationships among these systems for
Carrie O’Connor +7 more
doaj +2 more sources
Role of the Tryptophan Residues in Proton-Coupled Folate Transporter (PCFT-SLC46A1) Function [PDF]
American Journal of Physiology - Cell Physiology, 2016 The proton-coupled folate transporter (PCFT) mediates folate absorption across the brush-border membrane of the proximal small intestine and is required for folate transport across the choroid plexus into the cerebrospinal fluid. In this study, the functional role and accessibility of the seven PCFT Trp residues were assessed by the substituted ...
Andras Fiser +2 more
exaly +4 more sources
Biochemical and molecular mechanisms of folate transport in rat pancreas; interference with ethanol ingestion. [PDF]
PLoS ONE, 2011 Folic acid is an essential nutrient that is required for one-carbon biosynthetic processes and for methylation of biomolecules. Deficiency of this micronutrient leads to disturbances in normal physiology of cell.
Nissar Ahmad Wani +2 more
doaj +2 more sources
Identification and Functional Impact of Homo-Oligomers of the Human Proton-Coupled Folate Transporter [PDF]
Journal of Biological Chemistry, 2012 The proton-coupled folate transporter (PCFT; SLC46A1) is a proton-folate symporter that is abundantly expressed in solid tumors and normal tissues, such as duodenum. The acidic pH optimum for PCFT is relevant to intestinal absorption of folates and could afford a means of selectively targeting tumors with novel cytotoxic antifolates.
Zhanjun Hou +2 more
exaly +5 more sources
Reversible severe combined immunodeficiency phenotype secondary to a mutation of the proton-coupled folate transporter [PDF]
Clinical Immunology, 2009 Hereditary folate malabsorption is a rare inborn error of metabolism due to mutations in the proton-coupled folate transporter (PCFT). Clinical presentation of PCFT deficiency may mimic severe combined immune deficiency (SCID). We report a 4-month-old female who presented with failure to thrive, normocytic anemia, Pneumocystis jirovecii pneumonia and ...
Borzutzky A +7 more
openaire +6 more sources
The evolving biology of the proton-coupled folate transporter: New insights into regulation, structure, and mechanism. [PDF]
FASEB J, 2022 The human proton-coupled folate transporter (PCFT; SLC46A1) or hPCFT was identified in 2006 as the principal folate transporter involved in the intestinal absorption of dietary folates.
Hou Z, Gangjee A, Matherly LH.
europepmc +2 more sources
Development and validation of chemical features-based proton-coupled folate transporter/activity and reduced folate carrier/activity models (pharmacophores). [PDF]
J Mol Graph Model, 2018 All clinically used antifolates lack transport selectivity for tumors over normal cells resulting in dose-limiting toxicities. There is growing interest in developing novel tumor-targeted cytotoxic antifolates with selective transport into tumors over ...
Shah K +4 more
europepmc +2 more sources
Folate-appended cyclodextrin carrier targets ovarian cancer cells expressing the proton-coupled folate transporter. [PDF]
Cancer Sci, 2020 AbstractFolate receptor alpha (FRα) is overexpressed in >80% of epithelial ovarian cancer (EOC). Accordingly, folate is attracting attention as a targeting ligand for EOC. For EOC patients, paclitaxel (PTX) is generally used as a first‐line chemotherapeutic agent in combination with platinum‐based drugs.
Saito S +5 more
europepmc +4 more sources