Results 181 to 190 of about 3,956,989 (242)

Protoplast culture of perennials

Scientia Horticulturae, 1988
Abstract Hemerocallis and Musa clones grown under aseptic conditions provide valuable test systems wherein both morphological and biochemical prerequisites for expression of morphological competence from protoplasts can be investigated. While there is considerable opportunity to study protoplast isolation and regeneration per se, the greatest ...
A.D. Krikorian, Sandra S. Cronauer-Mitra, Mindy S.Fitter Corbin   +2 more
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Wheat protoplast culture: embryogenic colony formation from protoplasts

Plant Cell Reports, 1988
Wheat (Triticum aestivum L. cv Chinese Spring) protoplasts were isolated from immature embryos or embryogenic calli (3-4 weeks of culture on MS medium with 32 mg/1 dicamba) and cultured in R2 medium containing 2 mg/1 2,4-D by the nurse culture methods originally developed for rice protoplasts (Kyozuka et al. 1987). Protoplasts isolated from embryogenic
Y, Hayashi, K, Shimamoto
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Protoplast Culture of Hardwoods

1987
Hardwood trees, like most woody plants, present difficult challenges to the plant breeder. Not only are the plants unwieldy and thus difficult to manipulate in large numbers, but the life cycle with the attendant juvenile period of years to decades strongly limits the rapidity of improvement.
B. H. McCown, J. A. Russell
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Aspects of protoplast culture and plant regeneration

Plant Cell, Tissue and Organ Culture, 1988
Protoplasts are now used as source material for a range of genetic manipulations. Indeed, protoplast fusion has already produced somatic hybrid and cybrid plants, whilst techniques in molecular biology have advanced so rapidly that transgenic plants have now been reported.
M. R. Davey, J. B. Power
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Tissue Culture, Protoplasts, and Morphogenesis in Flax

Botanical Gazette, 1976
Morphogenesis in flax (Linum usitatissimum L `Redwing') was studied in hypocotyl sections taken 3-5 days after removal of the cotyledons and shoots and the sections were cultured on a Murashige and Skoog (1962) medium Zeatin and other cytokinins at 1-10 μM enhanced bud initiation and shoot development on the hypocotyl explants.
Gamborg, O. L., Shyluk, J. P.
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Regeneration of tobacco and petunia plants from protoplasts an culture of corn protoplasts

In Vitro, 1974
Protoplasts isolated enzymatically from leaves of tobacco (Nicotiana tabacum), stem callus of petunia (Petunia hybrida) and roots of corn (Zea mays) seedings were grown aseptically in microchambers, liquid droplets, or in stationary suspension cultures. Cell wall regenration occurred within 2 days in petunia, 6 to 7 days in tobacco, and 10 days in corn
V, Vasil, I K, Vasil
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Oxidative metabolism and protoplast culture

In Vitro Cellular & Developmental Biology - Plant, 1993
Barley leaf blade protoplasts accumulate malonaldehyde, a product of lipid peroxidation, during culture. In addition, glutathione levels fall after protoplast isolation and the proportion of glutathione in the oxidized state rises. These data indicate oxidative stress after protoplast isolation and during culture.
Kapur, Raman, Saleem, Mohammed, Harvey, Bryan L., Cutler, Adrian J.   +3 more
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Protoplasts: Isolation, culture, plant regeneration

1986
Publisher Summary This chapter presents an idealized example of protoplast isolation with the steps involved in protoplast isolation and culture; discusses the parameters involved in the developmental sequence from protoplast to plant; and overviews a collection of detailed laboratory protocols for protoplast isolation, clonal proliferation, and ...
INGO POTRYKUS, RAYMOND D. SHILLITO
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Isolation and Culture of Almond Protoplasts

Plant Science, 1985
Abstract Protoplasts were isolated enzymatically from suspension cultures of almond ( Prunus dulcis cv. Nonpareil). Under appropriate conditions, they regenerated cell walls, initiated cell division and proliferated into cell masses from which suspension cultures were re-established. Arginine and some polyamines were effective cell division inducers.
S.C. Wu, A.H. Kuniyuki
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Protoplast Isolation and Culture

2018
This chapter presents the techniques and approaches used in most studies of protoplast isolation and culture. The isolation of protoplasts from plant cells was first achieved by microsurgery on plasmolyzed cells. The plant species, the conditions of plant growth, plant age, protoplast isolation, and protoplast culture are often critical for sustained ...
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