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ISOLATION AND CULTURE OF TOMATO MESOPHYLL PROTOPLASTS
Acta Botanica Neerlandica, 1976SUMMARY Viable tomato mesophyll protoplasts were isolated by using the non-phytotoxic macerating enzyme PATE instead of the generally used Macerozyme. To obtain the osmotic conditions required during isolation, salt solutions, containing 2.5% (w/v) KCl and 1% (w/v) MgSO4 ยท 7H2O, were used.
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Protoplast Isolation and Culture
1995Protoplasts can be isolated from plant tissues or cultured cells by enzymatic digestion to remove the cell walls. The enzymes for this purpose are preparations which are commercially available. The success of protoplast isolation depends especially on the condition of the tissue and the combination of enzymes being used. There is no standard method for
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1973
This chapter discusses the plant protoplast culture. Protoplasts have been isolated from both intact plants and cell cultures of many plant species. Leaves are an especially suitable cell source because mesophyll cells have relatively thin walls which are readily degraded.
Miller, R.A., Kao, K.N., Gamborg, O.L.
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This chapter discusses the plant protoplast culture. Protoplasts have been isolated from both intact plants and cell cultures of many plant species. Leaves are an especially suitable cell source because mesophyll cells have relatively thin walls which are readily degraded.
Miller, R.A., Kao, K.N., Gamborg, O.L.
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Isolation and Culture of Citrus Protoplasts
1989Citrus fruits rank second only to grapes in world fruit production. Citrus production was estimated in 1985 at a total of approximately 60 million metric tons (FAO 1985). Oranges comprise the most important Citrus product in world trade, followed by grapefruit, mandarins (which have recently increased, especially on the European market) and lemons.
Aliza Vardi, Esra Galun
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Isolation and Culture of Potato Protoplasts
1989Potato, Solarium tuberosum, is one of the most important crops in the temperate climate zones. It occupies the fifth position among the cultivated plant species with respect to yield and the fourth position as measured by area of cultivation (Barocka and Ross 1985).
M. Zuba, H. Binding
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Isolation and Culture of Glycine Protoplasts
1989The soybean, Glycine max (L.) Merr., first emerged as a domesticated plant in the eastern half of north China around the eleventh century B. C, according to historical, linguistic and geographical evidence (Hymowitz 1970). By the first century A. D., it had probably spread through central and south China and into Korea; from there it expanded into ...
C. A. Newell, H. T. Luu
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Isolation and culture of grapevine protoplasts
2015VITIS - Journal of Grapevine Research, Vol. 29 (1990): Vitis (Special Issue)
Reustle, G., Alleweldt, G.
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Isolation, culture, and regeneration of protoplasts
1995Abstract Protoplasts provide the starting point for many of the techniques of genetic manipulation of plants, in particular the induction of somaclonal (protoclonal) variation, somatic hybridization, and transformation. Such experiments can be considered to consist essentially of three stages; protoplast isolation, the genetic ...
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