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Protoplast Isolation and Transformation in Oil Palm
, 2022 The protocol outlined in this chapter describes a detailed procedure for protoplast isolation and transformation using polyethylene glycol (PEG)-mediated transfection and DNA microinjection, highlighting also the critical steps associated with the method. Briefly, we will describe the efficient isolation of protoplasts from 3-month-old suspension calli
Mat Yunus Abdul, Masani +5 more
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Protoplast isolation of callus in Echinacea augustifolia
Colloids and Surfaces B: Biointerfaces, 2005Preparations from Echinacea are among the most widely used herbal medicines. Most uses of Echinacea are based on the reported immunological properties. In this paper, we used callus of Echinacea augustifolia for isolation and researched the factors influencing the process of protoplasts preparation, the result indicated it was easy to isolate ...
Bochu Wang, Chuanren Duan
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Protoplast Isolation and Transfection in Rice
2022Rice (Oryza sativa) is an important cereal crop and a model monocot plant for biology research. The reliable system of foreign DNA transformation and expression is a valuable strategy for basic research and molecular breeding application in rice. The Agrobacterium tumefaciens-mediated foreign DNA transformation system was a powerful tool for genetic ...
Zhaohui, Zhong, Yong, Zhang
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Protoplast Isolation and Transfection in Maize
2022Protoplast-based transient gene expression platforms can be used to study a range of questions concerning gene regulation. Crucial to the success of these studies is the isolation of large quantities of healthy protoplasts from the tissue of interest.
Monique R, Coy +2 more
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Reuse of enzymes for isolation of protoplasts
Plant Cell Reports, 1985A method has been developed for the reuse of cell wall digesting enzymes to isolate protoplasts from actively-growing suspension cultures of plant cells. Protoplasts could be satisfactorily prepared as many as three times using the same enzyme mixture without any loss in yield or viability of the isolated protoplasts. The yields of nuclei isolated from
P K, Saxena, J, King
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Fusion of Isolated Plant Protoplasts
Nature, 1970A first step towards somatic hybridization in plants has been achieved. Protoplasts have been isolated and seen to fuse.
J B, Power, S E, Cummins, E C, Cocking
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The isolation of barley-aleurone protoplasts
Planta, 1971A procedure is described for the isolation of large numbers of viable aleurone protoplasts. After treatment with "Onozuka" cellulase protoplasts were obtained which were surrounded by a thin, "Onozuka"-resistant wall. These cells were termed "spheroplasts".
L, Taiz, R L, Jones
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ISOLATION OF SUNFLOWER PROTOPLASTS
Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии, 2021Протопласты растений представляют собой отправную точку многих методик для генетической модификации целых растений и в настоящее время широко используются в качестве исходного материала для генетических манипуляций с культурными растениями. Несмотря на значительные успехи в технологии получения протопластов для ряда культурных растений, подсолнечник
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1992
Although protoplasts have been isolated from a range of somatic tissues and successfully cultured, the success in isolating the protoplasts from reproductive tissues has been rather limited. This is basically due to technical difficulties. In the pollen, for example, the exine, which is made up of sporopollenin, is resistant to enzymatic degradation ...
K. R. Shivanna, N. S. Rangaswamy
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Although protoplasts have been isolated from a range of somatic tissues and successfully cultured, the success in isolating the protoplasts from reproductive tissues has been rather limited. This is basically due to technical difficulties. In the pollen, for example, the exine, which is made up of sporopollenin, is resistant to enzymatic degradation ...
K. R. Shivanna, N. S. Rangaswamy
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Isolation and regeneration of protoplasts from Ectocarpus.
Cold Spring Harbor protocols, 2012This article describes how to obtain isolated cells with no surrounding cell wall by enzymatic digestion of Ectocarpus filaments. The resultant protoplasts are totipotent and regenerate to produce individual algae under appropriate culture conditions.
Coelho, S. +6 more
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