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Isolation and Transplantation of Nuclei into Plant Protoplasts
2003Plant protoplasts can internalize a variety of particles ranging from molecules (e.g., ferritin) to entire (algal) protoplasts, a size rangeof 0.09-23 µm (1). This capacity to take up foreign bodies provides a powerful tool with which to investigate the functions of cell organelles.
P K, Saxena, J, King
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Enzymes for the Isolation of Protoplasts
1989Cocking (1960) first used enzymes to isolate higher plant protoplasts. He applied culture filtrates of a cellulolytic fungus Myrothecium verrucaria to release protoplasts from tomato root tips. Later, Takebe and co-workers succeeded in applying commercially available cell wall-degrading enzymes, Macerozyme and Cellulase Onozuka, to isolate large ...
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Isolation of Allium pollen protoplasts
Plant Cell, Tissue and Organ Culture, 1992Studies on protoplasts isolation were carried out with mature pollen grains of 29 samples of species of Allium aflatunense, A. cepa, A. fistulosum, A. karataviense, A. longicuspis, A. nutans, A. odorum, A. sativum and A. schoenoprasum. Surface sterilized pollen grains drifted from crushed anthers were incubated in an enzyme solution containing 1% (w/v)
Martin Fellner, Pavel Havr�nek
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Fungal Protoplasts: Isolation, Reversion, and Fusion
Annual Review of Microbiology, 1979INTRODUCTION 21 ISOLATION OF PROTOPLASTS AND THEIR P ROPERTIES 22 Enzymatic Methods for Protoplast Isolation 22 Nonenzymatic Procedures for Protoplast Release 26 Properties of Protopiasts 27 WALL REGENERATION AND REVERSION OF PROTOPLASTS 28 Frequency of Protoplast Reversion 28 Morphology of Protoplast Reversion 29 Ultrastructure and ...
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Isolation and Culture of Citrus Protoplasts
1989Citrus fruits rank second only to grapes in world fruit production. Citrus production was estimated in 1985 at a total of approximately 60 million metric tons (FAO 1985). Oranges comprise the most important Citrus product in world trade, followed by grapefruit, mandarins (which have recently increased, especially on the European market) and lemons.
Aliza Vardi, Esra Galun
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Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture (Switzerland), 2021Junaid Iftikhar +2 more
exaly
Isolation, culture, and regeneration of protoplasts
1995Abstract Protoplasts provide the starting point for many of the techniques of genetic manipulation of plants, in particular the induction of somaclonal (protoclonal) variation, somatic hybridization, and transformation. Such experiments can be considered to consist essentially of three stages; protoplast isolation, the genetic ...
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