Results 221 to 230 of about 21,306 (282)

Integrated experimental and computational workflows for single-cell transcriptomics in plants. [PDF]

Plant Methods
Wang J, Zheng S, Lu B, Jiang Y, Zhu Y, Liu Q, Gao S, Liu P, Yu P, Jiang S, Zong L.   +10 more
europepmc   +1 more source

Protoplast-Based Functional Genomics and Genome Editing: Progress, Challenges and Applications. [PDF]

Plant Cell Environ
Hsieh JA, Wu FH, Yang DX, Wu AE, Liu CA, Chen CH, Lin SZ, Lin YJ, Lin CS.   +8 more
europepmc   +1 more source

Single-cell proteomics of Arabidopsis leaf mesophyll reveals dynamic protein responses to water-deficit stress. [PDF]

Genome Biol
Fulcher JM, Dawar P, Balasubramanian VK, Williams SM, Nadeau KD, Winkler TE, Markillie LM, Mitchell HD, Ahkami AH, Paša-Tolić L, Zhu Y.   +10 more
europepmc   +1 more source

Isolation and transformation of perennial ryegrass (<i>Lolium perenne</i> L.) protoplasts for the <i>in vivo</i> assessment of guide RNAs editing efficiency. [PDF]

Front Plant Sci
Sustek-Sánchez F, Eelmets E, Nigul L, Kärblane K, Laasmaa M, Balode-Sausina M, Berzina SA, Ducis D, Kaktina E, Jaškūne K, Rognli OA, Rostoks N, Sarmiento C.   +12 more
europepmc   +1 more source

Two Optimized Methods for Efficient, Stable and Transient Transformation of Broccoli (<i>Brassica oleracea</i> Var. Italica). [PDF]

Plants (Basel)
Coronado-Martín A, Atarés A, Porcel R, Yenush L, Mulet JM.   +4 more
europepmc   +1 more source

Droplet-based microfluidics platform for investigation of protoplast development of three exemplary plant species. [PDF]

Sci Rep
Marczakiewicz-Perera P, Winkelmann T, Köhler M, Cao J.   +3 more
europepmc   +1 more source

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Protoplast isolation of callus in Echinacea augustifolia

Colloids and Surfaces B: Biointerfaces, 2005
Preparations from Echinacea are among the most widely used herbal medicines. Most uses of Echinacea are based on the reported immunological properties. In this paper, we used callus of Echinacea augustifolia for isolation and researched the factors influencing the process of protoplasts preparation, the result indicated it was easy to isolate ...
Zhu, Liqing, Wang, Bochu, Zhou, Jing, Chen, Lingxi, Dai, Chuanyun, Duan, Chuanren   +5 more
exaly   +3 more sources

Protoplast Isolation and Transfection in Rice

2022
Rice (Oryza sativa) is an important cereal crop and a model monocot plant for biology research. The reliable system of foreign DNA transformation and expression is a valuable strategy for basic research and molecular breeding application in rice. The Agrobacterium tumefaciens-mediated foreign DNA transformation system was a powerful tool for genetic ...
Zhaohui, Zhong, Yong, Zhang
openaire   +2 more sources

Protoplast Isolation and Transfection in Maize

2022
Protoplast-based transient gene expression platforms can be used to study a range of questions concerning gene regulation. Crucial to the success of these studies is the isolation of large quantities of healthy protoplasts from the tissue of interest.
Monique R, Coy, Shane E, Abbitt, Mary J, Frank   +2 more
openaire   +2 more sources