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Protoplast Isolation and Transfection in Wheat
2022Wheat is one of the major staple crops around the world. A transient expression system is crucial for gene functional studies in wheat as stable transfection is still difficult in most cultivars. Protoplasts could serve as a versatile transient expression tool in wheat research.
Luo, Guangbin, Li, Boshu, Gao, Caixia
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Fusion of Isolated Plant Protoplasts
Nature, 1970A first step towards somatic hybridization in plants has been achieved. Protoplasts have been isolated and seen to fuse.
J B, Power, S E, Cummins, E C, Cocking
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The isolation of barley-aleurone protoplasts
Planta, 1971A procedure is described for the isolation of large numbers of viable aleurone protoplasts. After treatment with "Onozuka" cellulase protoplasts were obtained which were surrounded by a thin, "Onozuka"-resistant wall. These cells were termed "spheroplasts".
L, Taiz, R L, Jones
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Reuse of enzymes for isolation of protoplasts
Plant Cell Reports, 1985A method has been developed for the reuse of cell wall digesting enzymes to isolate protoplasts from actively-growing suspension cultures of plant cells. Protoplasts could be satisfactorily prepared as many as three times using the same enzyme mixture without any loss in yield or viability of the isolated protoplasts. The yields of nuclei isolated from
P K, Saxena, J, King
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Protoplast Isolation and Staining
2015The successful isolation of mesophyll protoplasts from plant species has become a versatile tool for in vivo imaging of subcellular structures. Taking advantage of the various cytochemical probes available, the subcellular localization of specific organelles can be visualized in live protoplasts.
Liu, MQ +6 more
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1992
Although protoplasts have been isolated from a range of somatic tissues and successfully cultured, the success in isolating the protoplasts from reproductive tissues has been rather limited. This is basically due to technical difficulties. In the pollen, for example, the exine, which is made up of sporopollenin, is resistant to enzymatic degradation ...
K. R. Shivanna, N. S. Rangaswamy
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Although protoplasts have been isolated from a range of somatic tissues and successfully cultured, the success in isolating the protoplasts from reproductive tissues has been rather limited. This is basically due to technical difficulties. In the pollen, for example, the exine, which is made up of sporopollenin, is resistant to enzymatic degradation ...
K. R. Shivanna, N. S. Rangaswamy
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Isolation and regeneration of protoplasts from Ectocarpus.
Cold Spring Harbor protocols, 2012This article describes how to obtain isolated cells with no surrounding cell wall by enzymatic digestion of Ectocarpus filaments. The resultant protoplasts are totipotent and regenerate to produce individual algae under appropriate culture conditions.
Coelho, S. +6 more
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ISOLATION OF SUNFLOWER PROTOPLASTS
Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии, 2021Протопласты растений представляют собой отправную точку многих методик для генетической модификации целых растений и в настоящее время широко используются в качестве исходного материала для генетических манипуляций с культурными растениями. Несмотря на значительные успехи в технологии получения протопластов для ряда культурных растений, подсолнечник
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The isolation, regeneration and fusion of Phycomyces protoplasts
Molecular and General Genetics MGG, 1974Germlings of Phycomyces blakesleeanus were converted to protoplasts using an enzyme solution of chitinase and chitosanase. The protoplasts were regenerated to normal growing mycelia. Protoplasts of different auxotrophic strains fused in diluted natural seawater and in Ca(NO3)2 solutions. 36 prototrophic strains were isolated (Table 1).
Binding, Horst, Weber, Hans Jürgen
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Isolation and Transfection of Maize Endosperm Protoplasts
2022Endosperm of cereal crops is the main component of its grain. Improvement of endosperm traits will bolster grain yield and quality. Functional analysis of endosperm trait-related genes often requires the use of an endosperm cell system. Here, we describe a protocol for the isolation and transfection of maize endosperm cell protoplast.
Yufeng, Hu, Yubi, Huang
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