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Enzymes for the Isolation of Protoplasts
1989Cocking (1960) first used enzymes to isolate higher plant protoplasts. He applied culture filtrates of a cellulolytic fungus Myrothecium verrucaria to release protoplasts from tomato root tips. Later, Takebe and co-workers succeeded in applying commercially available cell wall-degrading enzymes, Macerozyme and Cellulase Onozuka, to isolate large ...
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Fungal Protoplasts: Isolation, Reversion, and Fusion
Annual Review of Microbiology, 1979INTRODUCTION 21 ISOLATION OF PROTOPLASTS AND THEIR P ROPERTIES 22 Enzymatic Methods for Protoplast Isolation 22 Nonenzymatic Procedures for Protoplast Release 26 Properties of Protopiasts 27 WALL REGENERATION AND REVERSION OF PROTOPLASTS 28 Frequency of Protoplast Reversion 28 Morphology of Protoplast Reversion 29 Ultrastructure and ...
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Isolation and Culture of Glycine Protoplasts
1989The soybean, Glycine max (L.) Merr., first emerged as a domesticated plant in the eastern half of north China around the eleventh century B. C, according to historical, linguistic and geographical evidence (Hymowitz 1970). By the first century A. D., it had probably spread through central and south China and into Korea; from there it expanded into ...
C. A. Newell, H. T. Luu
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Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture (Switzerland), 2021Mohamed A A Ahmed +2 more
exaly