Results 241 to 250 of about 21,306 (282)

Protoplast Isolation and Transformation in Oil Palm

2022
The protocol outlined in this chapter describes a detailed procedure for protoplast isolation and transformation using polyethylene glycol (PEG)-mediated transfection and DNA microinjection, highlighting also the critical steps associated with the method. Briefly, we will describe the efficient isolation of protoplasts from 3-month-old suspension calli
Mat Yunus Abdul, Masani, Ghulam Kadir Ahmad, Parveez, Gundula, Noll, M D Piji Mohd Al Akmarul, Fizree, Ravigadevi, Sambanthamurthi, Dirk, Pruefer   +5 more
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Isolation of protoplasts in a case of enterococcal endocarditis

The American Journal of Medicine, 1968
Abstract The isolation of protoplasts from the aortic valve in a case of Streptococcus faecalis endocarditis is reported. The patient was successfully treated by excision of the valve without serious complications. The possible role of protoplasts in human disease is discussed.
H C, Neu, B, Goldreyer
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Isolation of Protoplasts from Pollen Tetrads

Nature New Biology, 1972
PRODUCTION of haploid plants by anther culture is restricted to only a few taxa1. If protoplasts could be isolated from pollen tetrads they might behave in culture similarly to somatic cell protoplasts2 and serve as the starting material for the production of haploid plants for a wide range of plant species.
S S, Bhojwani, E C, Cocking
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Isolation and Transplantation of Nuclei into Plant Protoplasts

2003
Plant protoplasts can internalize a variety of particles ranging from molecules (e.g., ferritin) to entire (algal) protoplasts, a size rangeof 0.09-23 µm (1). This capacity to take up foreign bodies provides a powerful tool with which to investigate the functions of cell organelles.
P K, Saxena, J, King
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Isolation of protoplasts from cereal leaves

Planta, 1972
Mature leaves of Secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (Meicelase) and pectinase (Pectinol R10) produced quantities of protoplasts. Under the same conditions leaves of Triticum aestivum, Hordeum vulgare and Avena sativa also produce protoplasts but in lower yields.
P K, Evans, A G, Keates, E C, Cocking
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Protoplast Isolation and Culture

2018
This chapter presents the techniques and approaches used in most studies of protoplast isolation and culture. The isolation of protoplasts from plant cells was first achieved by microsurgery on plasmolyzed cells. The plant species, the conditions of plant growth, plant age, protoplast isolation, and protoplast culture are often critical for sustained ...
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Protoplasts: Isolation, culture, plant regeneration

1986
Publisher Summary This chapter presents an idealized example of protoplast isolation with the steps involved in protoplast isolation and culture; discusses the parameters involved in the developmental sequence from protoplast to plant; and overviews a collection of detailed laboratory protocols for protoplast isolation, clonal proliferation, and ...
INGO POTRYKUS, RAYMOND D. SHILLITO
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Protoplast Isolation and Culture

1995
Protoplasts can be isolated from plant tissues or cultured cells by enzymatic digestion to remove the cell walls. The enzymes for this purpose are preparations which are commercially available. The success of protoplast isolation depends especially on the condition of the tissue and the combination of enzymes being used. There is no standard method for
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ISOLATION AND CULTURE OF TOMATO MESOPHYLL PROTOPLASTS

Acta Botanica Neerlandica, 1976
SUMMARY Viable tomato mesophyll protoplasts were isolated by using the non-phytotoxic macerating enzyme PATE instead of the generally used Macerozyme. To obtain the osmotic conditions required during isolation, salt solutions, containing 2.5% (w/v) KCl and 1% (w/v) MgSO4 · 7H2O, were used.
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Isolation and culture of almond protoplasts

Plant Science, 1985
Abstract Protoplasts were isolated enzymatically from suspension cultures of almond ( Prunus dulcis cv. Nonpareil). Under appropriate conditions, they regenerated cell walls, initiated cell division and proliferated into cell masses from which suspension cultures were re-established. Arginine and some polyamines were effective cell division inducers.
S.C. Wu, A.H. Kuniyuki
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