Results 121 to 130 of about 25,575 (276)

Analysis of Multiple Isoenzyme Expression Among Twenty-Two Species of Mycoplasma and Acholeplasma [PDF]

, 1981
Crude extracts of triple-cloned, purified cultures of 22 species of Mycoplasma and Acholeplasma were examined for expression of 21 isozyme systems routinely used to type mammalian cells. Nine previously described enzymes (purine nucleoside phosphorylase,
Barile, Michael F., Grabowski, Marion W., O\u27Brien, Stephen J., Simonson, Janice M.   +3 more
core   +1 more source

Challenges of drug resistance in the management of pancreatic cancer [PDF]

, 2010
The current treatment of choice for metastatic pancreatic cancer involves single agent gemcitabine or combination of gemcitabine with capecitabine and erlotinib (tyrosine kinase inhibitor).
Akada, Allison, Almoguera, Andreetta, Arai, Arlt, Arteaga, Arumugam, Asano, Bachem, Baldwin, Banerjee, Bartsch, Bergers, Best, Bold, Bondar, Buck, Burris, Chakravarti, Chakravarti, Choi, Cunningham, Cunningham, Dancer, Dong, Duxbury, Duxbury, Duxbury, Duxbury, Engelman, Feldmann, Ferrero, Galmarini, Geyer, Giovannetti, Goan, Greer, Hagmann, Hahn, Henke, Hruban, Humeniuk, Ischenko, Ishikawa, Itoi, Jansen, Karapetis, Kindler, Kong, Koopman, Kulik, Lei, Lemoine, Liau, Liau, Longley, Mackey, Mackey, Mackey, Mahon, Mamaghani, Marechal, Marmor, Martin Clynes, Mata, Maxwell, Mendelsohn, Meropol, Miwa, Moertel, Molina-Arcas, Moore, Morgillo, Moriyama, Muerkoster, Muerkoster, Nakahira, Nakajima, Nakano, Naomi Walsh, Nishimura, Oguri, Okamoto, Okumura, Olive, O’Driscoll, Pao, Park, Park, Peters, Plunkett, Ray McDermott, Ritzel, Rizwan Sheikh, Robert O’Connor, Ruggeri, Safran, Saito, Sakamoto, Schuller, Sebastiani, Shah, Shi, Simon, Spratlin, Takao, Takebayashi, Treat, Tsukamoto, Tzeng, Vaishampayan, Van Cutsem, van der Wilt, Wang, Wang, Wang, Xiong, Yamasaki, Yokoi   +119 more
core   +1 more source

Nucleotide degradation and ribose salvage in yeast

Molecular Systems Biology, 2013
Nucleotide degradation is a universal metabolic capability. Here we combine metabolomics, genetics and biochemistry to characterize the yeast pathway.
Yi‐Fan Xu, Fabien Létisse, Farnaz Absalan, Wenyun Lu, Ekaterina Kuznetsova, Greg Brown, Amy A Caudy, Alexander F Yakunin, James R Broach, Joshua D Rabinowitz   +9 more
doaj   +1 more source

Pinpointing dynamic coupling in enzymes for efficient drug design [PDF]

, 2016
Enzymes are proteins that catalyze almost every chemical reaction in living systems, achieving rate enhancements of up to 21 orders of magnitude relative to the uncatalyzed reactions.
Glasstone S, Haldane JBS, Polanyi M
core   +1 more source

An Efficient Chemoenzymatic Process for Preparation of Ribavirin

International Journal of Chemical Engineering, 2015
Ribavirin is an important antiviral drug, which is used for treatment of many diseases. The pilot-scale chemoenzymatic process for synthesis of the active pharmaceutical ingredient Ribavirin was developed with 32% overall yield and more than 99.5% purity.
Vladimir Sakharov, Sergey Baykov, Irina Konstantinova, Roman Esipov, Mikhail Dorogov   +4 more
doaj   +1 more source

Site-Selective Ribosylation of Fluorescent Nucleobase Analogs Using Purine-Nucleoside Phosphorylase as a Catalyst: Effects of Point Mutations

Molecules, 2015
Enzymatic ribosylation of fluorescent 8-azapurine derivatives, like 8-azaguanine and 2,6-diamino-8-azapurine, with purine-nucleoside phosphorylase (PNP) as a catalyst, leads to N9, N8, and N7-ribosides.
Alicja Stachelska-Wierzchowska, Jacek Wierzchowski, Agnieszka Bzowska, Beata Wielgus-Kutrowska   +3 more
doaj   +1 more source

In-silico gene essentiality analysis of polyamine biosynthesis reveals APRT as a potential target in cancer

, 2017
Constraint-based modeling for genome-scale metabolic networks has emerged in the last years as a promising approach to elucidate drug targets in cancer.
Agirre, Xabier, Apaolaza, Iñigo, Atauri, Pedro de, Carracedo, Arkaitz, Cascante i Serratosa, Marta, Cendoya, Xabier, Garate, Leire, Miranda, Estíbaliz, Ochoa, María Carmen, Pey, Jon, Planes, Francisco J., Prosper, Felipe, Rubio, Angel, San José-Eneriz, Edurne   +13 more
core   +3 more sources

Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36 [PDF]

, 2010
A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 was cloned and overexpressed in Escherichia coli. The enzyme was purified fivefold by membrane filtration and ion exchange.
A Bzowska, A Bzowska, A Bzowska, A Prasad, C Ge, C Mao, C Mao, D Balzer, Daniel F. Visser, Dean Brady, E Lewkowicz, Fritha Hennessy, G Dandanell, G Koellner, H Takami, JA Montgomery, Konanani Rashamuse, M Crampton, MA Larkin, Maureen E. Louw, MD Erion, ME Louw, MJ Pugmire, N Hori, N Hori, N Hori, N Hori, PP Saunders, PS Lovett, R Medici, R Schuch, S Rocchietti, SE Ealick, SF Altschul, SR Williams, SV Narayana, T Hamamoto, T Hamamoto, T Hamamoto, T Utagawa   +39 more
core   +2 more sources

Purine nucleoside phosphorylase from Schistosoma mansoni in complex with ribose-1-phosphate [PDF]

, 2011
A binary complex between a low-molecular-weight purine nucleoside phosphorylase and ribose-1-phosphate is described for the first time and comparisons with known ternary complexes are ...
D’Muniz Pereira, Humberto, Garratt, Richard Charles, Oliva, Glaucius   +2 more
core   +2 more sources

The clinical diagnostic value of a study of the activity of enzymes of the guanyl branch of purine metabolism in patients with ankylosing spondyloarthritis

Терапевтический архив, 2010
Aim. To assess the diagnosis of the activity of a pathological process in patients with ankylosing spondyloarthritis (AS) and to reveal purine metabolic (PM) changes in relation to the clinical features of AS. Materials and methods.
Aleksandr Borisovich Zborovskiy, Elena Eduardovna Mozgovaya, Vladislav Fedorovich Martem'yanov, Ol'ga Petrovna Slyusar', Mikhail Yur'evich Stazharov, S A Bedina, A B Zborovsky, E E Mozgovaya, V F Martemyanov, O P Slyusar, M Yu Stazharov, S A Bedina   +11 more
doaj