Results 71 to 80 of about 19,457 (231)

Engineering Substrate Promiscuity of Nucleoside Phosphorylase Via an Insertions–Deletions Strategy

JACS Au
Nucleoside phosphorylases (NPs) are the key enzymes in the nucleoside metabolism pathway and are widely employed for the synthesis of nucleoside analogs, which are difficult to access via conventional synthetic methods.
Gaofei Liu, Jialing Wang, Jianlin Chu, Tianyue Jiang, Song Qin, Zhen Gao, Bingfang He   +6 more
semanticscholar   +1 more source

Metabolic Origin, Role and Fate of the Denaturant Guanidine

Microbial Biotechnology, Volume 18, Issue 11, November 2025.
The origin of metabolic guanidine is largely a mystery. We suggest it is created when guanine‐containing nucleotides are oxidised by molecular oxygen instead of being broken down into urea as purines normally would. Guanidine may act as a signal to help cells control the level of reactive oxygen species.
Antoine Danchin, Victor de Lorenzo, Pablo Iván Nikel, Conghui You   +3 more
wiley   +1 more source

Cardiomyocyte‐Specific Deletion of Sirtuin 5 Accelerates the Development of Heart Failure Upon Dysregulating Purine Metabolism

Acta Physiologica, Volume 241, Issue 11, November 2025.
ABSTRACT Aim Sirtuin 5 (SIRT5), a mitochondrial NAD+‐dependent deacylase, regulates fundamental cellular pathways, including energy substrate metabolism. The current study is designed to better elucidate the role of SIRT5 in the development of heart failure (HF). Methods Mice with cardiomyocyte‐specific deletion (cSirt5−/−) or overexpression (cSirt5‐Tg)
Nikole J. Byrne, Christoph Koentges, Katharina Pfeil, Julia C. Lueg, Sayan Bakshi, Aleksandre Tarkhnishvili, Ivan Vosko, Johannes Gollmer, Laura C. Birkle, Thomas Rathner, Stephan Birkle, Sibai Tang, Clara Rau, Michael M. Hoffmann, Katja E. Odening, Stephen Barnes, Landon Shay Wilson, Senka Ljubojevic‐Holzer, Markus Wallner, Dirk von Lewinski, Peter Rainer, Simon Sedej, Harald Sourij, Christoph Bode, Adam R. Wende, Andreas Zirlik, Heiko Bugger   +26 more
wiley   +1 more source

The Role of the Intestinal Microbiome in the Pathogenesis and Treatment of Hyperuricemia: A Review

Food Science &Nutrition, Volume 13, Issue 10, October 2025.
This review summarizes the characteristics, prevalence, and treatment strategies of hyperuricemia, and subsequently examines its pathogenic mechanisms together with the potential roles and regulatory pathways of the gut microbiota in its alleviation.
Junyu Yang, Jiali Chen, Zhenmin Liu, Yezhi Qu, Xiqing Yue, Bo Yuan, Mohan Li   +6 more
wiley   +1 more source

Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs

FEBS Open Bio, 2015
Mycoplasmas may colonize tumor tissue in patients. The cytostatic activity of gemcitabine was dramatically decreased inMycoplasma hyorhinis‐infected tumor cell cultures compared with non‐infected tumor cell cultures.
Johan Vande Voorde, Peter Vervaeke, Sandra Liekens, Jan Balzarini   +3 more
doaj   +1 more source

Apolipoprotein M (ApoM) Ameliorates Acute Alcohol Intoxication (AAI)‐Hemorrhagic Shock and Resuscitation (HSR)‐Induced Microvascular Leakage

Microcirculation, Volume 32, Issue 7, October 2025.
ABSTRACT Objective Microvascular hyperpermeability is a serious complication that occurs from hemorrhagic shock and resuscitation (HSR), especially when combined with acute alcohol intoxication (AAI). We tested the hypothesis that administration of Apolipoprotein M (ApoM), a lipocalin that normally resides in plasma high‐density lipoproteins (HDL) and ...
Mengmeng Chang, Jerome W. Breslin
wiley   +1 more source

A specific adenosine phosphorylase, distinct from purine nucleoside phosphorylase

FEBS Letters, 1976
Several enzymes, catalyzing the reversible phosphorolysis of purine nucleosides have been described in eucaryotic cells and in micro-organisms [l-6] . The best known is the ‘purine nucleoside phosphorylase’ (EC 2.4.2.1) acting on the nucleosides of hypoxanthine and guanine. Adenosine is not used as substrate by this enzyme [6-81.
SENESI, SONIA, FALCONE, GIUSEPPE, MURA, UMBERTO, SGARRELLA F, IPATA, PIERO LUIGI   +4 more
openaire   +5 more sources

Metabolic Stress‐Induced Choline Kinase α (CHKA) Activation in Endothelial Subpopulation Contributes to Diabetes‐Associated Microvascular Dysfunction

Advanced Science, Volume 12, Issue 33, September 4, 2025.
This study uncovers CHKA as a pivotal driver of vascular dysfunction in diabetic retinopathy and highlights its role in endothelial dysfunction through NAD⁺‐SIRT1‐Notch signaling. Single‐cell and functional analyses, combined with clinical and genetic validation, suggest CHKA as a promising therapeutic target for the prevention and treatment of ...
Ling Ren, Linyu Zhang, Yun Bai, Chang Huang, Xiaosa Li, Fanfei Ma, Wan Mu, Mudi Yao, Chang Jiang, Xiangjun Chen, Qin Jiang, Biao Yan   +11 more
wiley   +1 more source

N‐ to C‐Glycoside Rearrangement of Uridine 5′‐Phosphate in Two Enzymatic Steps for the Production of Pseudouridine 5′‐Phosphate

Biotechnology and Bioengineering, Volume 122, Issue 9, Page 2456-2464, September 2025.
The authors present a streamlined cascade reaction for one‐pot N‐C rearrangement of uridine‐5′‐phosphate (UMP) to pseudouridine‐5′‐phosphate (ΨMP; a key mRNA vaccine building block), in two enzymatic steps catalyzed by UMP nucleosidase (EC 3.2.2.10) and ΨMP C‐glycosidase (EC 4.2.1.70).
Martin Pfeiffer, Franziska Guld, Bernd Nidetzky   +2 more
wiley   +1 more source

Variation in Gene Expression Across Infection Status and Elevation in a Hawaiian Honeycreeper

Ecology and Evolution, Volume 15, Issue 9, September 2025.
Introduced pathogens exert novel selection on hosts, and some hosts have adapted to highly virulent pathogens. We tested for differential gene expression between (i) avian malaria‐infected and ‐uninfected Hawai'i 'amakihi and (ii) low‐ and high‐elevation 'amakihi.
Loren Cassin‐Sackett, Katherine M. McClure, Taylor E. Callicrate, Eben H. Paxton, Robert C. Fleischer   +4 more
wiley   +1 more source