Results 51 to 60 of about 1,475 (148)

Genetic Code Expansion in Mammalian Cells [PDF]

, 2019
Proteins in nature are synthesized from a conservative set of 20 canonical amino acids, limiting the chemical space of biological systems. Over the last few decades, scientists have developed methods to expand the genetic code of living organisms ...
Beranek, Vaclav
core   +1 more source

Unraveling Synthetase's Mode of Action: The Pyrrolysyl‐tRNA Synthetase Dimer Uses Secondary Binding Sites in the Cell

Angewandte Chemie International Edition, EarlyView.
The pyrrolysyl‐tRNA synthetase dimer exhibits an alternating mode of action of its monomers within its catalytic cycle, which is realized upon occupation of secondary binding sites located at the intermonomer interfaces outside the catalytic binding site.
Jessica Dröden, Christoph Globisch, Eliane Landwehr, Theresa S. Braun, Daniel Summerer, Christine Peter, Malte Drescher   +6 more
wiley   +1 more source

Comparative evaluation of noncanonical amino acids as site‐specific NMR probes for the complex of E. coli SSB with single‐stranded DNA without isotope labelling

The FEBS Journal, EarlyView.
Genetic encoding of noncanonical amino acids (ncAA) enables their site‐specific installation in proteins. This work compares the nuclear magnetic resonance (NMR) performance of different ncAAs in a large protein−DNA complex (~ 100 kDa) without isotope labelling. The ncAAs deliver readily detectable NMR signals in the complex between E.
Sreelakshmi Mekkattu Tharayil, Haocheng Qianzhu, Elwy H. Abdelkader, Adarshi P. Welegedara, Josemon George, Christoph Nitsche, Thomas Huber, Gottfried Otting   +7 more
wiley   +1 more source

Genetic code expansion in neurons for studying pathogenic mechanisms of Alzheimer disease [PDF]

, 2022
Every three seconds a new patient is diagnosed with Alzheimer disease (AD). The primary risk factor for this irreversible, progressive brain disorder is old age.
van Husen, Lea Susanne
core   +1 more source

Genetic Encoding of a Trifunctional Photo‐Cross‐Linker with a Cleavable Alkyl Ester Moiety

ChemBioChem, Volume 27, Issue 3, 12 February 2026.
A novel noncanonical amino acid, DiZAAsu, possessing photo‐cross‐linking, biotinylation, and alkaline‐cleavable functionalities, is genetically encoded by an engineered pyrrolyl‐tRNA synthetase mutant. The alkyl ester moiety expands the design space of cleavable photo‐cross‐linkable amino acids.
Masahiro Takayama, Tomoya Tsubota, Takao Yamaguchi, Kosuke Chiba, Takumi Yoshida, Yoshiyuki Hari, Yu‐Shi Tian, Daisuke Takaya, Asuka Mori, Tomohito Tsukamoto, Kenji Ishimoto, Yukio Ago, Yoshiaki Okada, Kensaku Sakamoto, Takefumi Doi, Kaori Fukuzawa, Satoshi Obika, Shinsaku Nakagawa, Nobumasa Hino   +18 more
wiley   +1 more source

A Genetically Encoded Homocysteine Precursor to Probe Protein Active Sites and to Addict Escherichia coli to a Noncanonical Amino Acid Directly Involved in Catalysis

Angewandte Chemie, Volume 137, Issue 36, September 1, 2025.
A new genetically encoded amino acid is chemically converted into homocysteine (Hcy) under mild conditions to probe and to activate protein active sites. Surprisingly, Hcy can replace cysteine or serine residues in several enzymes. Its potential for a widespread use in proteins is further underlined by rendering Escherichia coli dependent on Hcy ...
Clara Dunker, Henning D. Mootz
wiley   +2 more sources

Synthetic biology approaches to biological containment: pre-emptively tackling potential risks [PDF]

, 2016
Biocontainment comprises any strategy applied to ensure that harmful organisms are confined to controlled laboratory conditions and not allowed to escape into the environment.
Csibra, E., Gianni, E., Kruger, A., Pinheiro, Vitor B., Torres, L.   +4 more
core   +1 more source

An Expanded Genetic Code In Mammalian Cells With A Functional Quadruplet Codon [PDF]

, 2013
We have utilized in vitro evolution to identify tRNA variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to a quadruplet codon in both bacterial and mammalian cells.
Guo, Jiantao, Niu, Wei, Schultz, Peter G.   +2 more
core   +2 more sources

Genetische Codierung von Pentafluorphosphato‐Phenylalanin ermöglicht die Herstellung von PF5‐Proteinen als Phosphoprotein‐Mimetika

Angewandte Chemie, Volume 138, Issue 1, 2 January 2026.
Erweiterung des genetischen Codes mit einem einzigartigen hyperfluorierten Phosphotyrosin‐Analogon. In dieser Arbeit haben wir die nicht‐natürliche Aminosäure Pentafluorphosphato‐Difluormethyl‐Phenylalanin, die sieben Fluoratome und eine permanente negative Ladung trägt, mittels mutierter, orthogonaler Aminoacyl‐tRNA‐Synthetasen erfolgreich in drei ...
Anna Magdalena Ambros, Haocheng Qianzhu, Markus Tiemann, Edan Habel, Katrin Denzinger, Hana Zupan, Matteo Accorsi, Bettina G. Keller, Gerhard Wolber, Thomas Huber, Jörg Rademann   +10 more
wiley   +1 more source

Tuning the Functionality of Designer Translating Organelles with Orthogonal tRNA Synthetase/tRNA Pairs [PDF]

Site-specific incorporation of noncanonical amino acids (ncAAs) can be realized by genetic code expansion (GCE) technology. Different orthogonal tRNA synthetase/tRNA (RS/tRNA) pairs have been developed to introduce a ncAA at the desired site, delivering ...
Jung, M. , Lemke, E., Sushkin, M.
core   +2 more sources