Results 21 to 30 of about 23,834 (239)
Analyzing clonal variation of monoclonal antibody-producing CHO cell lines using an in silico metabolomic platform. [PDF]
PLoS ONE, 2014 Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins.
Atefeh Ghorbaniaghdam +3 more
doaj +1 more source
RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease
Hepatology, EarlyView., 2022 RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabolic liver disease. Abstract Background and Aims Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD)
Marta B. Afonso +16 more
wiley +1 more source
Pyruvate Carboxylase in Rhodopseudomonas spheroides [PDF]
Journal of General Microbiology, 1969 SUMMARY: Unlike some other photosynthetic bacteria, Rhodopseudomonas spheroides directly carboxylates pyruvate in a reaction catalysed by a pyruvate carboxylase (E.C. 6·4.1·1). A partially purified preparation of the enzyme was acetylCoA-dependent. No phosphoenolpyruvate synthetase or phosphoenolpyruvate carboxylase activity was detected in extracts of
J. Payne, J. G. Morris
openaire +3 more sources
Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells
Nature Communications, 2023 Deregulated oxidative metabolism is a hallmark of leukaemia. While tyrosine kinase inhibitors (TKIs) such as imatinib have increased survival of chronic myeloid leukaemia (CML) patients, they fail to eradicate disease-initiating leukemic stem cells (LSCs)
Kevin M. Rattigan +18 more
doaj +1 more source
AIMS Bioengineering, 2016 Glycerol is a major by-product of the biodiesel industry and its transformation into value-added products is an ongoing technological challenge. Here we report on the ability of the nutritionally-versatile Pseudomonas fluorescens to synthesize copious ...
Azhar A. Alhasawi, Vasu D. Appanna
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2021 Wild-type C. glutamicum ATCC 13032 is known to possess two enzymes with anaplerotic (C4-directed) carboxylation activity, namely phosphoenolpyruvate carboxylase (PEPCx) and pyruvate carboxylase (PCx). On the other hand, C3-directed decarboxylation can be
Jannick Kappelmann +8 more
doaj +1 more source
Frontiers in Physiology, 2019 It is hypothesized that glucokinase (GCK) is the glucose sensor not only for regulation of insulin release by pancreatic β-cells, but also for the rest of the cells that contribute to glucose homeostasis in mammals. This includes other cells in endocrine
Franz M. Matschinsky, David F. Wilson
doaj +1 more source
The Metabolic Fates of Pyruvate in Normal and Neoplastic Cells
Cells, 2021 Pyruvate occupies a central metabolic node by virtue of its position at the crossroads of glycolysis and the tricarboxylic acid (TCA) cycle and its production and fate being governed by numerous cell-intrinsic and extrinsic factors.
Edward V. Prochownik, Huabo Wang
doaj +1 more source
Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production
Microbial Cell Factories, 2023 Background Pyruvate is a widely used value-added chemical which also serves as a hub of various metabolic pathways. The fastest-growing bacterium Vibrio natriegens is a promising chassis for synthetic biology applications with high substrate uptake rates.
Fengli Wu +4 more
doaj +1 more source
Pyruvate carboxylase deficiency: An underestimated cause of lactic acidosis
Molecular Genetics and Metabolism Reports, 2015 Pyruvate carboxylase (PC) is a biotin-containing mitochondrial enzyme that catalyzes the conversion of pyruvate to oxaloacetate, thereby being involved in gluconeogenesis and in energy production through replenishment of the tricarboxylic acid (TCA ...
F. Habarou +13 more
doaj +1 more source